A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells
Daucus carota L. ssp. carota (Apiaceae), also known as wild carrot, is a commonly used medicinal plant in folk medicine worldwide for the treatment of several ailments including cancer [1]. Earlier studies in our labs revealed that the acetone: methanol oil extract of the plant possesses potent anti...
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| Other Authors: | , , , , |
| Format: | article |
| Published: |
2016
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| Online Access: | http://hdl.handle.net/10725/7421 http://dx.doi.org/10.1055/s-0036-1596492 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0036-1596492 |
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| Summary: | Daucus carota L. ssp. carota (Apiaceae), also known as wild carrot, is a commonly used medicinal plant in folk medicine worldwide for the treatment of several ailments including cancer [1]. Earlier studies in our labs revealed that the acetone: methanol oil extract of the plant possesses potent anticancer activity against a panel of acute myeloid leukemia (AML) cells [2]. The aim of the present study was to isolate the active compound(s) and evaluate its anticancer activity. Successive column chromatography and fractionation led to the isolation of a new sesquiterpene unique to the Lebanese wild carrot, identified as 1β-2-himachalen-6-ol using GC-MS and 1D and 2D NMR spectroscopy. The anticancer activity of 1β-2-himachalen-6-ol was evaluated against two AML cell line, HL60 and ML2. Results revealed that the IC50 of the two latter cells were 21 and 15 µg/mL, corresponding to 94.5 and 67.5µM, respectively. Cell cycle analysis using flow cytometry revealed an increase in the sub-G1 population. Analysis of cell death after treatment with different molecule concentrations showed that cells were positive for Annexin V, PI, and active caspase staining, indicating a caspase dependent apoptotic cell death. In conclusion, 1β-2-himachalen-6-ol possesses potent anticancer activity against the tested AML cells, an effect mediated via caspase dependent apoptotic cell death. |
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