A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells
Daucus carota L. ssp. carota (Apiaceae), also known as wild carrot, is a commonly used medicinal plant in folk medicine worldwide for the treatment of several ailments including cancer [1]. Earlier studies in our labs revealed that the acetone: methanol oil extract of the plant possesses potent anti...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | , , , , |
| التنسيق: | article |
| منشور في: |
2016
|
| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/7421 http://dx.doi.org/10.1055/s-0036-1596492 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0036-1596492 |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| _version_ | 1864513466218315776 |
|---|---|
| author | Boulos, J. |
| author2 | Abi-Habib, R. Shehaby, W. Daher, CF Taleb, RI Mroueh, MA |
| author2_role | author author author author author |
| author_facet | Boulos, J. Abi-Habib, R. Shehaby, W. Daher, CF Taleb, RI Mroueh, MA |
| author_role | author |
| dc.creator.none.fl_str_mv | Boulos, J. Abi-Habib, R. Shehaby, W. Daher, CF Taleb, RI Mroueh, MA |
| dc.date.none.fl_str_mv | 2016 2018-04-19T08:17:56Z 2018-04-19T08:17:56Z 2018-04-19 |
| dc.identifier.none.fl_str_mv | 1439-0221 http://hdl.handle.net/10725/7421 http://dx.doi.org/10.1055/s-0036-1596492 Boulos, J., Abi-Habib, R., El-Sibai, M., Shebaby, W., Daher, C. F., Taleb, R. I., & Mroueh, M. A. (2016). A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells. Planta Medica, 82(S 01), P380. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0036-1596492 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Planta Medica |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Daucus carota L. ssp. carota (Apiaceae), also known as wild carrot, is a commonly used medicinal plant in folk medicine worldwide for the treatment of several ailments including cancer [1]. Earlier studies in our labs revealed that the acetone: methanol oil extract of the plant possesses potent anticancer activity against a panel of acute myeloid leukemia (AML) cells [2]. The aim of the present study was to isolate the active compound(s) and evaluate its anticancer activity. Successive column chromatography and fractionation led to the isolation of a new sesquiterpene unique to the Lebanese wild carrot, identified as 1β-2-himachalen-6-ol using GC-MS and 1D and 2D NMR spectroscopy. The anticancer activity of 1β-2-himachalen-6-ol was evaluated against two AML cell line, HL60 and ML2. Results revealed that the IC50 of the two latter cells were 21 and 15 µg/mL, corresponding to 94.5 and 67.5µM, respectively. Cell cycle analysis using flow cytometry revealed an increase in the sub-G1 population. Analysis of cell death after treatment with different molecule concentrations showed that cells were positive for Annexin V, PI, and active caspase staining, indicating a caspase dependent apoptotic cell death. In conclusion, 1β-2-himachalen-6-ol possesses potent anticancer activity against the tested AML cells, an effect mediated via caspase dependent apoptotic cell death. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_ba6e4ea18985beaffa1593f47c9b3243 |
| identifier_str_mv | 1439-0221 Boulos, J., Abi-Habib, R., El-Sibai, M., Shebaby, W., Daher, C. F., Taleb, R. I., & Mroueh, M. A. (2016). A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells. Planta Medica, 82(S 01), P380. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/7421 |
| publishDate | 2016 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cellsBoulos, J.Abi-Habib, R.Shehaby, W.Daher, CFTaleb, RIMroueh, MADaucus carota L. ssp. carota (Apiaceae), also known as wild carrot, is a commonly used medicinal plant in folk medicine worldwide for the treatment of several ailments including cancer [1]. Earlier studies in our labs revealed that the acetone: methanol oil extract of the plant possesses potent anticancer activity against a panel of acute myeloid leukemia (AML) cells [2]. The aim of the present study was to isolate the active compound(s) and evaluate its anticancer activity. Successive column chromatography and fractionation led to the isolation of a new sesquiterpene unique to the Lebanese wild carrot, identified as 1β-2-himachalen-6-ol using GC-MS and 1D and 2D NMR spectroscopy. The anticancer activity of 1β-2-himachalen-6-ol was evaluated against two AML cell line, HL60 and ML2. Results revealed that the IC50 of the two latter cells were 21 and 15 µg/mL, corresponding to 94.5 and 67.5µM, respectively. Cell cycle analysis using flow cytometry revealed an increase in the sub-G1 population. Analysis of cell death after treatment with different molecule concentrations showed that cells were positive for Annexin V, PI, and active caspase staining, indicating a caspase dependent apoptotic cell death. In conclusion, 1β-2-himachalen-6-ol possesses potent anticancer activity against the tested AML cells, an effect mediated via caspase dependent apoptotic cell death.PublishedN/A2018-04-19T08:17:56Z2018-04-19T08:17:56Z20162018-04-19Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1439-0221http://hdl.handle.net/10725/7421http://dx.doi.org/10.1055/s-0036-1596492Boulos, J., Abi-Habib, R., El-Sibai, M., Shebaby, W., Daher, C. F., Taleb, R. I., & Mroueh, M. A. (2016). A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells. Planta Medica, 82(S 01), P380.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0036-1596492enPlanta Medicainfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/74212024-01-30T13:41:53Z |
| spellingShingle | A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells Boulos, J. |
| status_str | publishedVersion |
| title | A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells |
| title_full | A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells |
| title_fullStr | A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells |
| title_full_unstemmed | A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells |
| title_short | A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells |
| title_sort | A new sesquiterpene from Lebanese wild carrot inhibits proliferation of human acute myeloid leukemia cells |
| url | http://hdl.handle.net/10725/7421 http://dx.doi.org/10.1055/s-0036-1596492 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0036-1596492 |