Skin responses to fractional photothermolysis
Background and Objective Fractional photothermolysis (FP) is a new concept using arrays of microscopic thermal damage patterns to stimulate a therapeutic response. We analyzed epidermal and dermal response to FP with the aim of correlating histological and clinical response. Study Design/Materials a...
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2006
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| Online Access: | http://hdl.handle.net/10725/6533 http://dx.doi.org/10.1002/lsm.20254 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1002/lsm.20254/full |
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| _version_ | 1864513480188493824 |
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| author | Tannous, Zeina |
| author2 | Laubach, Hans-Joachim Anderson, Rox |
| author2_role | author author |
| author_facet | Tannous, Zeina Laubach, Hans-Joachim Anderson, Rox |
| author_role | author |
| dc.creator.none.fl_str_mv | Tannous, Zeina Laubach, Hans-Joachim Anderson, Rox |
| dc.date.none.fl_str_mv | 2006 2017-11-08T07:30:37Z 2017-11-08T07:30:37Z 2017-11-08 |
| dc.identifier.none.fl_str_mv | 1096-9101 http://hdl.handle.net/10725/6533 http://dx.doi.org/10.1002/lsm.20254 Laubach, H. J., Tannous, Z., Anderson, R., & Manstein, D. (2006). Skin responses to fractional photothermolysis. Lasers in surgery and medicine, 38(2), 142-149. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1002/lsm.20254/full |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Lasers in Surgery and Medicine |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Skin responses to fractional photothermolysis |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Background and Objective Fractional photothermolysis (FP) is a new concept using arrays of microscopic thermal damage patterns to stimulate a therapeutic response. We analyzed epidermal and dermal response to FP with the aim of correlating histological and clinical response. Study Design/Materials and Methods Twelve subjects received a single treatment with a prototype diode laser emitting at a wavelength of 1,500 nm, delivering 5 mJ per microscopic treatment zone (MTZ), and a density of 1,600 MTZs/cm2 on the forearm. Biopsies were procured over a period of 3 months. The biopsies were analyzed by two blinded dermatopathologists using hematoxylin and eosin (Hematoxylin and Eosin Stain), Elastica von Gieson, nitro-blue-tetrazolium-chloride (NBTC) viability, and immunohistochemistry stains. Furthermore, the treatment sites were evaluated in vivo by confocal microscopy. Results and Discussion Twenty-four hours after fractional photothermolysis, the continuity of the epidermal basal cell layer is restored. Complete epidermal regeneration is obtained 7 days after the treatment. Microscopic epidermal necrotic debris (MENDs) are seen as early as 1 day after FP. MENDs contain melanin pigment, and are shed from the epidermis within 7 days. Evidence of increased collagen III production is shown with immunohistochemistry (IHC) staining 7 days after FP. IHC for heat shock protein 70 (HSP 70) shows the expression of HSP 1 day after FP, and IHC for alpha smooth muscle actin shows the presence of myofibroblasts 7 days after FP. These findings are concordant with the induction of a wound healing response by FP. There is no evidence of residual dermal fibrosis 3 months after treatment. Conclusion A single treatment with fractional photothermolysis induces a wound healing response in the dermis. A mechanism for the precise removal of epidermal melanin is described, in which MENDs act as a melanin shuttle. Lasers Surg. Med. 38:142–149, 2006. © 2006 Wiley-Liss, Inc. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_bac3d00b7d1d8765f3e6df0baaf0b088 |
| identifier_str_mv | 1096-9101 Laubach, H. J., Tannous, Z., Anderson, R., & Manstein, D. (2006). Skin responses to fractional photothermolysis. Lasers in surgery and medicine, 38(2), 142-149. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6533 |
| publishDate | 2006 |
| repository.mail.fl_str_mv | |
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| spelling | Skin responses to fractional photothermolysisTannous, ZeinaLaubach, Hans-JoachimAnderson, RoxBackground and Objective Fractional photothermolysis (FP) is a new concept using arrays of microscopic thermal damage patterns to stimulate a therapeutic response. We analyzed epidermal and dermal response to FP with the aim of correlating histological and clinical response. Study Design/Materials and Methods Twelve subjects received a single treatment with a prototype diode laser emitting at a wavelength of 1,500 nm, delivering 5 mJ per microscopic treatment zone (MTZ), and a density of 1,600 MTZs/cm2 on the forearm. Biopsies were procured over a period of 3 months. The biopsies were analyzed by two blinded dermatopathologists using hematoxylin and eosin (Hematoxylin and Eosin Stain), Elastica von Gieson, nitro-blue-tetrazolium-chloride (NBTC) viability, and immunohistochemistry stains. Furthermore, the treatment sites were evaluated in vivo by confocal microscopy. Results and Discussion Twenty-four hours after fractional photothermolysis, the continuity of the epidermal basal cell layer is restored. Complete epidermal regeneration is obtained 7 days after the treatment. Microscopic epidermal necrotic debris (MENDs) are seen as early as 1 day after FP. MENDs contain melanin pigment, and are shed from the epidermis within 7 days. Evidence of increased collagen III production is shown with immunohistochemistry (IHC) staining 7 days after FP. IHC for heat shock protein 70 (HSP 70) shows the expression of HSP 1 day after FP, and IHC for alpha smooth muscle actin shows the presence of myofibroblasts 7 days after FP. These findings are concordant with the induction of a wound healing response by FP. There is no evidence of residual dermal fibrosis 3 months after treatment. Conclusion A single treatment with fractional photothermolysis induces a wound healing response in the dermis. A mechanism for the precise removal of epidermal melanin is described, in which MENDs act as a melanin shuttle. Lasers Surg. Med. 38:142–149, 2006. © 2006 Wiley-Liss, Inc.PublishedN/A2017-11-08T07:30:37Z2017-11-08T07:30:37Z20062017-11-08Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1096-9101http://hdl.handle.net/10725/6533http://dx.doi.org/10.1002/lsm.20254Laubach, H. J., Tannous, Z., Anderson, R., & Manstein, D. (2006). Skin responses to fractional photothermolysis. Lasers in surgery and medicine, 38(2), 142-149.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://onlinelibrary.wiley.com/doi/10.1002/lsm.20254/fullenLasers in Surgery and Medicineinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/65332021-03-19T10:03:27Z |
| spellingShingle | Skin responses to fractional photothermolysis Tannous, Zeina |
| status_str | publishedVersion |
| title | Skin responses to fractional photothermolysis |
| title_full | Skin responses to fractional photothermolysis |
| title_fullStr | Skin responses to fractional photothermolysis |
| title_full_unstemmed | Skin responses to fractional photothermolysis |
| title_short | Skin responses to fractional photothermolysis |
| title_sort | Skin responses to fractional photothermolysis |
| url | http://hdl.handle.net/10725/6533 http://dx.doi.org/10.1002/lsm.20254 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1002/lsm.20254/full |