Plasma amino acids vs conventional predictors of insulin resistance measured by the hyperinsulinemic clamp
Context: Specific plasma amino acid (AA) profiles including elevated postabsorptive branched-chain amino acids (BCAAs) have been associated with insulin resistance (IR), mostly estimated by homeostatic model assessment. This study assessed the associations of postabsorptive AAs with IR directly meas...
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| مؤلفون آخرون: | , , , , |
| التنسيق: | article |
| منشور في: |
2017
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/7790 https://doi.org/10.1210/js.2016-1108 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://academic.oup.com/jes/article/1/7/861/3762427 |
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| _version_ | 1864513467041447936 |
|---|---|
| author | Bassil, Maya |
| author2 | Labonte, Cherise C. Farsijani, Samaneh Winter, Aaron Murphy, Jessica Pereira, Sandra |
| author2_role | author author author author author |
| author_facet | Bassil, Maya Labonte, Cherise C. Farsijani, Samaneh Winter, Aaron Murphy, Jessica Pereira, Sandra |
| author_role | author |
| dc.creator.none.fl_str_mv | Bassil, Maya Labonte, Cherise C. Farsijani, Samaneh Winter, Aaron Murphy, Jessica Pereira, Sandra |
| dc.date.none.fl_str_mv | 2017 2018-05-10T07:45:23Z 2018-05-10T07:45:23Z 2018-05-10 |
| dc.identifier.none.fl_str_mv | 2472-1972 http://hdl.handle.net/10725/7790 https://doi.org/10.1210/js.2016-1108 Labonte, C. C., Farsijani, S., Marliss, E. B., Gougeon, R., Morais, J. A., Pereira, S., ... & Chevalier, S. (2017). Plasma Amino Acids vs Conventional Predictors of Insulin Resistance Measured by the Hyperinsulinemic Clamp. Journal of the Endocrine Society, 1(7), 861-873. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://academic.oup.com/jes/article/1/7/861/3762427 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of the Endocrine Society |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Plasma amino acids vs conventional predictors of insulin resistance measured by the hyperinsulinemic clamp |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Context: Specific plasma amino acid (AA) profiles including elevated postabsorptive branched-chain amino acids (BCAAs) have been associated with insulin resistance (IR), mostly estimated by homeostatic model assessment. This study assessed the associations of postabsorptive AAs with IR directly measured by insulin-mediated glucose disposal and determined the quantitative value of AAs and conventional IR predictors. Design: Fifty-one healthy, 31 overweight or obese (Ow/Ob), and 52 men and women with type 2 diabetes (T2D) were studied retrospectively. The main outcome measures were the glucose disposal (M/I) index (using 3-[3H]-glucose) during a hyperinsulinemic–euglycemic clamp and whole-body protein turnover (using 1-[13C]-leucine). Results: Compared with healthy participants, M/I was lower in Ow/Ob participants and lowest in those with T2D. BCAAs, glutamate, and lysine were higher in the Ow/Ob and T2D groups than in healthy participants; glycine and threonine were lower. Most AAs were higher in men. Principal component analysis identified component 1 (C1: BCAAs, methionine) and C3 (glycine, threonine, serine). Glutamate, C1, ornithine, lysine, methionine, and tyrosine correlated negatively with M/I; C3 and glycine correlated positively. Waist circumference and sex strongly influenced AA–IR relationships; only glutamate correlated after these factors were controlled for. From regression analysis, waist circumference, fasting glucose, insulin, and free fatty acids (FFAs) negatively predicted 64% of the M/I variance; glutamate added 2% more. In nondiabetic participants, IR was predicted by waist circumference, insulin, and FFAs, without contribution from AAs. Conclusion: Several postabsorptive AAs correlated with IR but added limited predictive value to conventional markers because levels were determined largely by abdominal adiposity. Data suggest a sex-specific regulation of AA metabolism by excess adiposity, particularly the BCAAs, warranting investigation. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_baf959fa6ed979f12d641b0d930e4951 |
| identifier_str_mv | 2472-1972 Labonte, C. C., Farsijani, S., Marliss, E. B., Gougeon, R., Morais, J. A., Pereira, S., ... & Chevalier, S. (2017). Plasma Amino Acids vs Conventional Predictors of Insulin Resistance Measured by the Hyperinsulinemic Clamp. Journal of the Endocrine Society, 1(7), 861-873. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/7790 |
| publishDate | 2017 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Plasma amino acids vs conventional predictors of insulin resistance measured by the hyperinsulinemic clampBassil, MayaLabonte, Cherise C.Farsijani, SamanehWinter, AaronMurphy, JessicaPereira, SandraContext: Specific plasma amino acid (AA) profiles including elevated postabsorptive branched-chain amino acids (BCAAs) have been associated with insulin resistance (IR), mostly estimated by homeostatic model assessment. This study assessed the associations of postabsorptive AAs with IR directly measured by insulin-mediated glucose disposal and determined the quantitative value of AAs and conventional IR predictors. Design: Fifty-one healthy, 31 overweight or obese (Ow/Ob), and 52 men and women with type 2 diabetes (T2D) were studied retrospectively. The main outcome measures were the glucose disposal (M/I) index (using 3-[3H]-glucose) during a hyperinsulinemic–euglycemic clamp and whole-body protein turnover (using 1-[13C]-leucine). Results: Compared with healthy participants, M/I was lower in Ow/Ob participants and lowest in those with T2D. BCAAs, glutamate, and lysine were higher in the Ow/Ob and T2D groups than in healthy participants; glycine and threonine were lower. Most AAs were higher in men. Principal component analysis identified component 1 (C1: BCAAs, methionine) and C3 (glycine, threonine, serine). Glutamate, C1, ornithine, lysine, methionine, and tyrosine correlated negatively with M/I; C3 and glycine correlated positively. Waist circumference and sex strongly influenced AA–IR relationships; only glutamate correlated after these factors were controlled for. From regression analysis, waist circumference, fasting glucose, insulin, and free fatty acids (FFAs) negatively predicted 64% of the M/I variance; glutamate added 2% more. In nondiabetic participants, IR was predicted by waist circumference, insulin, and FFAs, without contribution from AAs. Conclusion: Several postabsorptive AAs correlated with IR but added limited predictive value to conventional markers because levels were determined largely by abdominal adiposity. Data suggest a sex-specific regulation of AA metabolism by excess adiposity, particularly the BCAAs, warranting investigation.PublishedN/A2018-05-10T07:45:23Z2018-05-10T07:45:23Z20172018-05-10Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2472-1972http://hdl.handle.net/10725/7790https://doi.org/10.1210/js.2016-1108Labonte, C. C., Farsijani, S., Marliss, E. B., Gougeon, R., Morais, J. A., Pereira, S., ... & Chevalier, S. (2017). Plasma Amino Acids vs Conventional Predictors of Insulin Resistance Measured by the Hyperinsulinemic Clamp. Journal of the Endocrine Society, 1(7), 861-873.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://academic.oup.com/jes/article/1/7/861/3762427enJournal of the Endocrine Societyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/77902021-03-19T10:03:31Z |
| spellingShingle | Plasma amino acids vs conventional predictors of insulin resistance measured by the hyperinsulinemic clamp Bassil, Maya |
| status_str | publishedVersion |
| title | Plasma amino acids vs conventional predictors of insulin resistance measured by the hyperinsulinemic clamp |
| title_full | Plasma amino acids vs conventional predictors of insulin resistance measured by the hyperinsulinemic clamp |
| title_fullStr | Plasma amino acids vs conventional predictors of insulin resistance measured by the hyperinsulinemic clamp |
| title_full_unstemmed | Plasma amino acids vs conventional predictors of insulin resistance measured by the hyperinsulinemic clamp |
| title_short | Plasma amino acids vs conventional predictors of insulin resistance measured by the hyperinsulinemic clamp |
| title_sort | Plasma amino acids vs conventional predictors of insulin resistance measured by the hyperinsulinemic clamp |
| url | http://hdl.handle.net/10725/7790 https://doi.org/10.1210/js.2016-1108 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://academic.oup.com/jes/article/1/7/861/3762427 |