Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in Rats
Aim: The study was designed to investigate the immediate (1 h) effect of leptin and ghrelin injection on in vivo postprandial hepatic glycogen and lipid synthesis. Animals andMethods: Adult Sprague-Dawley male rats were fed a semisynthetic control diet ad libitum. Overnight-fasted rats were gavaged...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2015
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/2759 http://dx.doi.org/10.1159/000100816 http://www.karger.com/Article/Abstract/100816 |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513459160350720 |
|---|---|
| author | Bassil, Maya S. |
| author2 | Mrayati, Maha M. Hwalla, Nahla C. Obeid, Omar A. |
| author2_role | author author author |
| author_facet | Bassil, Maya S. Mrayati, Maha M. Hwalla, Nahla C. Obeid, Omar A. |
| author_role | author |
| dc.creator.none.fl_str_mv | Bassil, Maya S. Mrayati, Maha M. Hwalla, Nahla C. Obeid, Omar A. |
| dc.date.none.fl_str_mv | 2015-12-04T09:44:43Z 2015-12-04T09:44:43Z 2015-12-04 |
| dc.identifier.none.fl_str_mv | 0250-6807 http://hdl.handle.net/10725/2759 http://dx.doi.org/10.1159/000100816 Bassil, M. S., Mrayati, M. M., Hwalla, N. C., & Obeid, O. A. (2007). Acute effect of leptin and ghrelin injection on postprandial glycogen and lipid synthesis in rats. Annals of Nutrition and Metabolism, 51(1), 14-21. http://www.karger.com/Article/Abstract/100816 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Annals of nutrition & metabolism |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in Rats |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Aim: The study was designed to investigate the immediate (1 h) effect of leptin and ghrelin injection on in vivo postprandial hepatic glycogen and lipid synthesis. Animals andMethods: Adult Sprague-Dawley male rats were fed a semisynthetic control diet ad libitum. Overnight-fasted rats were gavaged with 4 ml of water containing 1.25 g of the diet and immediately injected intraperitoneally with 4 mCi of 3H2O. After 1 h, rats were either intraperitoneally injected with saline (control), leptin (20 µg/rat) or ghrelin (10 µg/rat) and sacrificed 1 h later. Blood, liver and epididymal fat pads (EFP) were taken for analysis. Results: Plasma triglyceride level was higher in the leptin group compared to control. Leptin injection reduced hepatic glycogen synthesis while glycogen accumulation was not affected and ghrelin injection did not affect hepatic glycogen synthesis. Both hepatic and EFP lipogenesis were not affected by leptin or ghrelin. Conclusion: Leptin and ghrelin administration had no immediate effect on hepatic and adipose tissue lipogenesis. Leptin reduced in vivo postprandial hepatic glycogenesis and increased plasma triglyceride level which may be due to reduced uptake by peripheral tissues. Thus, leptin was found to exert an immediate effect on lipid and carbohydrate metabolism unlike that of ghrelin. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_bd7901420a2d0ab20554df35ab494c0f |
| identifier_str_mv | 0250-6807 Bassil, M. S., Mrayati, M. M., Hwalla, N. C., & Obeid, O. A. (2007). Acute effect of leptin and ghrelin injection on postprandial glycogen and lipid synthesis in rats. Annals of Nutrition and Metabolism, 51(1), 14-21. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/2759 |
| publishDate | 2015 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in RatsBassil, Maya S.Mrayati, Maha M.Hwalla, Nahla C.Obeid, Omar A.Aim: The study was designed to investigate the immediate (1 h) effect of leptin and ghrelin injection on in vivo postprandial hepatic glycogen and lipid synthesis. Animals andMethods: Adult Sprague-Dawley male rats were fed a semisynthetic control diet ad libitum. Overnight-fasted rats were gavaged with 4 ml of water containing 1.25 g of the diet and immediately injected intraperitoneally with 4 mCi of 3H2O. After 1 h, rats were either intraperitoneally injected with saline (control), leptin (20 µg/rat) or ghrelin (10 µg/rat) and sacrificed 1 h later. Blood, liver and epididymal fat pads (EFP) were taken for analysis. Results: Plasma triglyceride level was higher in the leptin group compared to control. Leptin injection reduced hepatic glycogen synthesis while glycogen accumulation was not affected and ghrelin injection did not affect hepatic glycogen synthesis. Both hepatic and EFP lipogenesis were not affected by leptin or ghrelin. Conclusion: Leptin and ghrelin administration had no immediate effect on hepatic and adipose tissue lipogenesis. Leptin reduced in vivo postprandial hepatic glycogenesis and increased plasma triglyceride level which may be due to reduced uptake by peripheral tissues. Thus, leptin was found to exert an immediate effect on lipid and carbohydrate metabolism unlike that of ghrelin.PublishedN/A2015-12-04T09:44:43Z2015-12-04T09:44:43Z2015-12-04Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0250-6807http://hdl.handle.net/10725/2759http://dx.doi.org/10.1159/000100816Bassil, M. S., Mrayati, M. M., Hwalla, N. C., & Obeid, O. A. (2007). Acute effect of leptin and ghrelin injection on postprandial glycogen and lipid synthesis in rats. Annals of Nutrition and Metabolism, 51(1), 14-21.http://www.karger.com/Article/Abstract/100816enAnnals of nutrition & metabolisminfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/27592016-08-12T11:17:45Z |
| spellingShingle | Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in Rats Bassil, Maya S. |
| status_str | publishedVersion |
| title | Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in Rats |
| title_full | Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in Rats |
| title_fullStr | Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in Rats |
| title_full_unstemmed | Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in Rats |
| title_short | Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in Rats |
| title_sort | Acute Effect of Leptin and Ghrelin Injection on Postprandial Glycogen and Lipid Synthesis in Rats |
| url | http://hdl.handle.net/10725/2759 http://dx.doi.org/10.1159/000100816 http://www.karger.com/Article/Abstract/100816 |