Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene
The Xba I polymorphic site in the factor VIII gene is present in the int22h-1 region which is found in two other copies (int22h-2 and int22h-3) distal to the gene. Previously the polymorphic status of the Xba I locus was studied by either Southern blot or PCR that amplified all three copies. Here we...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , |
| التنسيق: | article |
| منشور في: |
1999
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/6162 http://dx.doi.org/10.1046/j.1365-2141.1999.01430.x http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2141.1999.01430.x/full |
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| _version_ | 1864513478615629824 |
|---|---|
| author | El Maarri, Osman |
| author2 | Oldenburg, Johannes Caglayan, S.Hande |
| author2_role | author author |
| author_facet | El Maarri, Osman Oldenburg, Johannes Caglayan, S.Hande |
| author_role | author |
| dc.creator.none.fl_str_mv | El Maarri, Osman Oldenburg, Johannes Caglayan, S.Hande |
| dc.date.none.fl_str_mv | 1999 2017-09-12T06:49:24Z 2017-09-12T06:49:24Z 2017-09-12 |
| dc.identifier.none.fl_str_mv | 1365-2141 http://hdl.handle.net/10725/6162 http://dx.doi.org/10.1046/j.1365-2141.1999.01430.x El‐Maarri, O., Oldenburg, J., & Çağlayan, S. H. (1999). Intron 22‐specific long PCR for the Xba I polymorphism in the factor VIII gene. British journal of haematology, 105(4), 1120-1122. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2141.1999.01430.x/full |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | British journal of Haematology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The Xba I polymorphic site in the factor VIII gene is present in the int22h-1 region which is found in two other copies (int22h-2 and int22h-3) distal to the gene. Previously the polymorphic status of the Xba I locus was studied by either Southern blot or PCR that amplified all three copies. Here we report the use of a long PCR that specifically amplifies the intragenic site in intron 22, making use of this marker an easy and reliable assay. Moreover, about 25% of previously uninformative Turkish haemophilia A families examined with three markers proved to be informative for linkage analysis, using the Xba I polymorphism. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_bf1e7fcbb7924fcd40ef6e84fcd3ce1e |
| identifier_str_mv | 1365-2141 El‐Maarri, O., Oldenburg, J., & Çağlayan, S. H. (1999). Intron 22‐specific long PCR for the Xba I polymorphism in the factor VIII gene. British journal of haematology, 105(4), 1120-1122. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6162 |
| publishDate | 1999 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII geneEl Maarri, OsmanOldenburg, JohannesCaglayan, S.HandeThe Xba I polymorphic site in the factor VIII gene is present in the int22h-1 region which is found in two other copies (int22h-2 and int22h-3) distal to the gene. Previously the polymorphic status of the Xba I locus was studied by either Southern blot or PCR that amplified all three copies. Here we report the use of a long PCR that specifically amplifies the intragenic site in intron 22, making use of this marker an easy and reliable assay. Moreover, about 25% of previously uninformative Turkish haemophilia A families examined with three markers proved to be informative for linkage analysis, using the Xba I polymorphism.PublishedN/A2017-09-12T06:49:24Z2017-09-12T06:49:24Z19992017-09-12Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1365-2141http://hdl.handle.net/10725/6162http://dx.doi.org/10.1046/j.1365-2141.1999.01430.xEl‐Maarri, O., Oldenburg, J., & Çağlayan, S. H. (1999). Intron 22‐specific long PCR for the Xba I polymorphism in the factor VIII gene. British journal of haematology, 105(4), 1120-1122.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://onlinelibrary.wiley.com/doi/10.1046/j.1365-2141.1999.01430.x/fullenBritish journal of Haematologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/61622021-03-19T10:00:49Z |
| spellingShingle | Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene El Maarri, Osman |
| status_str | publishedVersion |
| title | Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene |
| title_full | Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene |
| title_fullStr | Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene |
| title_full_unstemmed | Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene |
| title_short | Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene |
| title_sort | Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene |
| url | http://hdl.handle.net/10725/6162 http://dx.doi.org/10.1046/j.1365-2141.1999.01430.x http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2141.1999.01430.x/full |