Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem
The main mechanism of imipenem resistance in Pseudomonas aeruginosa is via downregulation of the gene for OprD porin. In a previous study, it was shown that the level of resistance did not parallel with the degree of downregulation of the porin gene, thus arguing for the existence of other resistanc...
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| مؤلفون آخرون: | , , , |
| التنسيق: | article |
| منشور في: |
2008
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/10654 https://doi.org/10.1016/j.ijantimicag.2007.12.016 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.sciencedirect.com/science/article/pii/S0924857908000496#! |
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| _version_ | 1864513467457732608 |
|---|---|
| author | Farra, Anna |
| author2 | Islam, Sohidul Stralfors, Annelie Sorberg, Mikael Wretlind, Bengt |
| author2_role | author author author author |
| author_facet | Farra, Anna Islam, Sohidul Stralfors, Annelie Sorberg, Mikael Wretlind, Bengt |
| author_role | author |
| dc.creator.none.fl_str_mv | Farra, Anna Islam, Sohidul Stralfors, Annelie Sorberg, Mikael Wretlind, Bengt |
| dc.date.none.fl_str_mv | 2008 2019-05-23T09:03:24Z 2019-05-23T09:03:24Z 2019-05-23 |
| dc.identifier.none.fl_str_mv | 1872-7913 http://hdl.handle.net/10725/10654 https://doi.org/10.1016/j.ijantimicag.2007.12.016 Farra, A., Islam, S., Strålfors, A., Sörberg, M., & Wretlind, B. (2008). Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem. International journal of antimicrobial agents, 31(5), 427-433. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.sciencedirect.com/science/article/pii/S0924857908000496#! |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | International Journal of Antimicrobial Agents |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | The main mechanism of imipenem resistance in Pseudomonas aeruginosa is via downregulation of the gene for OprD porin. In a previous study, it was shown that the level of resistance did not parallel with the degree of downregulation of the porin gene, thus arguing for the existence of other resistance mechanisms. Penicillin-binding protein (PBP) 2 and PBP3 are involved in carbapenem resistance in Escherichia coli. The genes for PBPs were sequenced in three imipenem-resistant clinical strains and these strains were conjugated with two susceptible P. aeruginosa PA0 strains, selecting for auxotrophic markers. In all the clinical and resistant isolates there was no obvious elevation of AmpC cephalosporinase. The active sites of PBP1b (ponB), PBP2 (pbpA), PBP3 (pbpB) and PBP6 (dacC) had no mutations in any of the examined strains. Production of oprD mRNA was significantly lower in clinical strains and transconjugants after selection for the proB marker (PA4565 at 5113 kb). The clinical strains had alterations in OprD that were not found in transconjugants. Our findings suggest that PBPs do not play a role in imipenem resistance in the clinical strains examined here, but that a regulatory gene for oprD contributing to carbapenem resistance is located close to the proB gene. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_c388e20e5c3a2a490d152a150c04af7c |
| identifier_str_mv | 1872-7913 Farra, A., Islam, S., Strålfors, A., Sörberg, M., & Wretlind, B. (2008). Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem. International journal of antimicrobial agents, 31(5), 427-433. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/10654 |
| publishDate | 2008 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenemFarra, AnnaIslam, SohidulStralfors, AnnelieSorberg, MikaelWretlind, BengtThe main mechanism of imipenem resistance in Pseudomonas aeruginosa is via downregulation of the gene for OprD porin. In a previous study, it was shown that the level of resistance did not parallel with the degree of downregulation of the porin gene, thus arguing for the existence of other resistance mechanisms. Penicillin-binding protein (PBP) 2 and PBP3 are involved in carbapenem resistance in Escherichia coli. The genes for PBPs were sequenced in three imipenem-resistant clinical strains and these strains were conjugated with two susceptible P. aeruginosa PA0 strains, selecting for auxotrophic markers. In all the clinical and resistant isolates there was no obvious elevation of AmpC cephalosporinase. The active sites of PBP1b (ponB), PBP2 (pbpA), PBP3 (pbpB) and PBP6 (dacC) had no mutations in any of the examined strains. Production of oprD mRNA was significantly lower in clinical strains and transconjugants after selection for the proB marker (PA4565 at 5113 kb). The clinical strains had alterations in OprD that were not found in transconjugants. Our findings suggest that PBPs do not play a role in imipenem resistance in the clinical strains examined here, but that a regulatory gene for oprD contributing to carbapenem resistance is located close to the proB gene.PublishedN/A2019-05-23T09:03:24Z2019-05-23T09:03:24Z20082019-05-23Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1872-7913http://hdl.handle.net/10725/10654https://doi.org/10.1016/j.ijantimicag.2007.12.016Farra, A., Islam, S., Strålfors, A., Sörberg, M., & Wretlind, B. (2008). Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem. International journal of antimicrobial agents, 31(5), 427-433.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.sciencedirect.com/science/article/pii/S0924857908000496#!enInternational Journal of Antimicrobial Agentsinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/106542021-03-19T10:45:25Z |
| spellingShingle | Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem Farra, Anna |
| status_str | publishedVersion |
| title | Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem |
| title_full | Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem |
| title_fullStr | Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem |
| title_full_unstemmed | Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem |
| title_short | Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem |
| title_sort | Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem |
| url | http://hdl.handle.net/10725/10654 https://doi.org/10.1016/j.ijantimicag.2007.12.016 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.sciencedirect.com/science/article/pii/S0924857908000496#! |