Waterpipe Tobacco Smoke Nicotine Mediates Resilience to Chronic Stress by Activating BDNF Signaling
The purpose of this study was to investigate how nicotine in the context of waterpipe tobacco smoking (WTS) affects depression and anxiety-like behaviors associated with chronic social defeat stress (CSDS) in a male C57BL/6J animal model. The mice were exposed to WTS or received intraperitoneal inje...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| التنسيق: | masterThesis |
| منشور في: |
2020
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/13574 https://doi.org/10.26756/th.2022.274 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513468269330432 |
|---|---|
| author | Khalifeh, Mohamad Rabieh |
| author_facet | Khalifeh, Mohamad Rabieh |
| author_role | author |
| dc.creator.none.fl_str_mv | Khalifeh, Mohamad Rabieh |
| dc.date.none.fl_str_mv | 2020 2020-03-18 2022-05-17T11:57:46Z 2022-05-17T11:57:46Z |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10725/13574 https://doi.org/10.26756/th.2022.274 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |
| dc.language.none.fl_str_mv | en |
| dc.publisher.none.fl_str_mv | Lebanese American University |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Nicotine -- Physiological effect Nicotine -- Psychological aspects Smoking -- Psychological aspects Neurotrophic functions Stress (Psychology) -- Health aspects Lebanese American University -- Dissertations Dissertations, Academic |
| dc.title.none.fl_str_mv | Waterpipe Tobacco Smoke Nicotine Mediates Resilience to Chronic Stress by Activating BDNF Signaling |
| dc.type.none.fl_str_mv | Thesis info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/masterThesis |
| description | The purpose of this study was to investigate how nicotine in the context of waterpipe tobacco smoking (WTS) affects depression and anxiety-like behaviors associated with chronic social defeat stress (CSDS) in a male C57BL/6J animal model. The mice were exposed to WTS or received intraperitoneal injections of nicotine for thirty days, before being subjected to CSDS for ten days. During CSDS, mice were exposed to WTS or received nicotine injections. The social interaction and open-field tests were used to classify animals as resilient or susceptible to stress and to evaluate their anxiety-like behavior and social interaction. Mice post behavioral testing, were further exposed to WTS/nicotine for ten days, and their behavior was reassessed. The involvement of brain-derived neurotrophic factor signaling in the nicotine-mediated effects was assessed with the tropomyosin receptor kinase B (TRKB) inhibitor, ANA-12. The reported results indicated that WTS promoted resilience to stress and rescued social avoidance in the used mice model. Even though initially, WTS decreased anxiety-like behaviors it was found that prolonged exposure after the completion of CSDS significantly induced anxiety-like behaviors. Finally, we showed that nicotine mediates the effects of WTS only on resilience to stress by increasing BDNF and TRKB levels and signaling. Our results suggested that the pathways mediating resilience to stress and anxiety are distinct and that nicotine mediates the effects of WTS on social behavior, but not anxiety, by activating BDNF signaling. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | LAURepo_c588317cbc55b361b37903c9744e8f4c |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/13574 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Lebanese American University |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Waterpipe Tobacco Smoke Nicotine Mediates Resilience to Chronic Stress by Activating BDNF SignalingKhalifeh, Mohamad RabiehNicotine -- Physiological effectNicotine -- Psychological aspectsSmoking -- Psychological aspectsNeurotrophic functionsStress (Psychology) -- Health aspectsLebanese American University -- DissertationsDissertations, AcademicThe purpose of this study was to investigate how nicotine in the context of waterpipe tobacco smoking (WTS) affects depression and anxiety-like behaviors associated with chronic social defeat stress (CSDS) in a male C57BL/6J animal model. The mice were exposed to WTS or received intraperitoneal injections of nicotine for thirty days, before being subjected to CSDS for ten days. During CSDS, mice were exposed to WTS or received nicotine injections. The social interaction and open-field tests were used to classify animals as resilient or susceptible to stress and to evaluate their anxiety-like behavior and social interaction. Mice post behavioral testing, were further exposed to WTS/nicotine for ten days, and their behavior was reassessed. The involvement of brain-derived neurotrophic factor signaling in the nicotine-mediated effects was assessed with the tropomyosin receptor kinase B (TRKB) inhibitor, ANA-12. The reported results indicated that WTS promoted resilience to stress and rescued social avoidance in the used mice model. Even though initially, WTS decreased anxiety-like behaviors it was found that prolonged exposure after the completion of CSDS significantly induced anxiety-like behaviors. Finally, we showed that nicotine mediates the effects of WTS only on resilience to stress by increasing BDNF and TRKB levels and signaling. Our results suggested that the pathways mediating resilience to stress and anxiety are distinct and that nicotine mediates the effects of WTS on social behavior, but not anxiety, by activating BDNF signaling.1 online (x, 82 leaves) ; ill. (some col.)Includes bibliographical references (leaf 66-82)Lebanese American University2022-05-17T11:57:46Z2022-05-17T11:57:46Z20202020-03-18Thesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10725/13574https://doi.org/10.26756/th.2022.274http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.phpeninfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/135742022-07-07T06:46:54Z |
| spellingShingle | Waterpipe Tobacco Smoke Nicotine Mediates Resilience to Chronic Stress by Activating BDNF Signaling Khalifeh, Mohamad Rabieh Nicotine -- Physiological effect Nicotine -- Psychological aspects Smoking -- Psychological aspects Neurotrophic functions Stress (Psychology) -- Health aspects Lebanese American University -- Dissertations Dissertations, Academic |
| status_str | publishedVersion |
| title | Waterpipe Tobacco Smoke Nicotine Mediates Resilience to Chronic Stress by Activating BDNF Signaling |
| title_full | Waterpipe Tobacco Smoke Nicotine Mediates Resilience to Chronic Stress by Activating BDNF Signaling |
| title_fullStr | Waterpipe Tobacco Smoke Nicotine Mediates Resilience to Chronic Stress by Activating BDNF Signaling |
| title_full_unstemmed | Waterpipe Tobacco Smoke Nicotine Mediates Resilience to Chronic Stress by Activating BDNF Signaling |
| title_short | Waterpipe Tobacco Smoke Nicotine Mediates Resilience to Chronic Stress by Activating BDNF Signaling |
| title_sort | Waterpipe Tobacco Smoke Nicotine Mediates Resilience to Chronic Stress by Activating BDNF Signaling |
| topic | Nicotine -- Physiological effect Nicotine -- Psychological aspects Smoking -- Psychological aspects Neurotrophic functions Stress (Psychology) -- Health aspects Lebanese American University -- Dissertations Dissertations, Academic |
| url | http://hdl.handle.net/10725/13574 https://doi.org/10.26756/th.2022.274 http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php |