The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas
Leptin has a modulator effect on glucose-stimulated insulin secretion. To define the influences of different glucose (4, 8, 12, and 16 mmol/L) and leptin (5, 10, 15, and 20 nmol/L) concentrations on total insulin release in ex vivo pancreatic preparations, a customized perfusion technique was used....
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2006
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| Online Access: | http://hdl.handle.net/10725/2812 http://dx.doi.org/10.1139/y06-006 http://www.nrcresearchpress.com/doi/abs/10.1139/y06-006#.V44HR7h97y0 |
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| _version_ | 1864513459415154688 |
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| author | Daher, C. |
| author2 | Haddad, N. Howland, R. Baroody, G. |
| author2_role | author author author |
| author_facet | Daher, C. Haddad, N. Howland, R. Baroody, G. |
| author_role | author |
| dc.creator.none.fl_str_mv | Daher, C. Haddad, N. Howland, R. Baroody, G. |
| dc.date.none.fl_str_mv | 2006 2015-12-16T07:24:33Z 2015-12-16T07:24:33Z 2015-12-16 |
| dc.identifier.none.fl_str_mv | 0008-4212 http://hdl.handle.net/10725/2812 http://dx.doi.org/10.1139/y06-006 Haddad, N., Howland, R., Baroody, G., & Daher, C. (2006). The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas. Canadian journal of physiology and pharmacology, 84(2), 157-162. http://www.nrcresearchpress.com/doi/abs/10.1139/y06-006#.V44HR7h97y0 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Canadian Journal of Physiology and Pharmacology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Leptin has a modulator effect on glucose-stimulated insulin secretion. To define the influences of different glucose (4, 8, 12, and 16 mmol/L) and leptin (5, 10, 15, and 20 nmol/L) concentrations on total insulin release in ex vivo pancreatic preparations, a customized perfusion technique was used. Such a profile of concentration brought about an index for the combined effect of leptin and glucose on the production of insulin. Insulin output was measured by radioimmunoassay. Stimulated by glucose alone in the control group, insulin secretion confirmed a bi-phasic pattern. Addition of leptin in the experimental group suppressed insulin secretion compared with control. A U-shape pattern of suppression was observed when the leptin and stimulatory glucose concentrations were combined. At 12 mmol/L glucose, leptin showed maximal insulin suppression. Leptin’s effect on insulin was glucose dependent and showed a reproducible U-shaped pattern of suppression, which implicated possible direct dose-dependent interaction between leptin and glucose on insulin secretion. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_ccc1fb9543e83273795bdfba6983e5f0 |
| identifier_str_mv | 0008-4212 Haddad, N., Howland, R., Baroody, G., & Daher, C. (2006). The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas. Canadian journal of physiology and pharmacology, 84(2), 157-162. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/2812 |
| publishDate | 2006 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreasDaher, C.Haddad, N.Howland, R.Baroody, G.Leptin has a modulator effect on glucose-stimulated insulin secretion. To define the influences of different glucose (4, 8, 12, and 16 mmol/L) and leptin (5, 10, 15, and 20 nmol/L) concentrations on total insulin release in ex vivo pancreatic preparations, a customized perfusion technique was used. Such a profile of concentration brought about an index for the combined effect of leptin and glucose on the production of insulin. Insulin output was measured by radioimmunoassay. Stimulated by glucose alone in the control group, insulin secretion confirmed a bi-phasic pattern. Addition of leptin in the experimental group suppressed insulin secretion compared with control. A U-shape pattern of suppression was observed when the leptin and stimulatory glucose concentrations were combined. At 12 mmol/L glucose, leptin showed maximal insulin suppression. Leptin’s effect on insulin was glucose dependent and showed a reproducible U-shaped pattern of suppression, which implicated possible direct dose-dependent interaction between leptin and glucose on insulin secretion.PublishedN/A2015-12-16T07:24:33Z2015-12-16T07:24:33Z20062015-12-16Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0008-4212http://hdl.handle.net/10725/2812http://dx.doi.org/10.1139/y06-006Haddad, N., Howland, R., Baroody, G., & Daher, C. (2006). The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas. Canadian journal of physiology and pharmacology, 84(2), 157-162.http://www.nrcresearchpress.com/doi/abs/10.1139/y06-006#.V44HR7h97y0enCanadian Journal of Physiology and Pharmacologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/28122017-02-10T10:07:14Z |
| spellingShingle | The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas Daher, C. |
| status_str | publishedVersion |
| title | The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas |
| title_full | The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas |
| title_fullStr | The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas |
| title_full_unstemmed | The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas |
| title_short | The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas |
| title_sort | The modulatory effect of leptin on the overall insulin production in ex-vivo normal rat pancreas |
| url | http://hdl.handle.net/10725/2812 http://dx.doi.org/10.1139/y06-006 http://www.nrcresearchpress.com/doi/abs/10.1139/y06-006#.V44HR7h97y0 |