Diagnostic considerations for Novel Influenza A (H1N1)
Background: The emergence of novel swine-origin influenza A (H1N1) virus (S-OIV) infection represents a significant pandemic threat. Rapid and accurate diagnosis will enhance treatment and containment efforts. Methods: Ninety eight patients with influenza-like illness meeting the CDC’s guidelines fo...
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2012
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| Online Access: | http://hdl.handle.net/10725/3937 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.ijppsjournal.com/Vol4Suppl1/2931.pdf |
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| _version_ | 1864513462519988224 |
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| author | Sheikh-Taha, Marwan |
| author2 | Eiland III, Edward H. Han, Jian Lindgren, Willliam Macandrew, Thomas English, Ali Hassoun |
| author2_role | author author author author author |
| author_facet | Sheikh-Taha, Marwan Eiland III, Edward H. Han, Jian Lindgren, Willliam Macandrew, Thomas English, Ali Hassoun |
| author_role | author |
| dc.creator.none.fl_str_mv | Sheikh-Taha, Marwan Eiland III, Edward H. Han, Jian Lindgren, Willliam Macandrew, Thomas English, Ali Hassoun |
| dc.date.none.fl_str_mv | 2012 2016-06-02T13:08:34Z 2016-06-02T13:08:34Z 2016-06-02 |
| dc.identifier.none.fl_str_mv | Sheikh-Taha, M., Eiland E, Han J., Lindgren w., Macandrew English T., Hassoun A. (2012). Diagnostic considerations for novel influenza A (H1N1): International journal of pharmacy and pharmaceutical sciences,4 Suppl.1,73-75 0975-1491 http://hdl.handle.net/10725/3937 Sheikh-Taha, M., Eiland III, E. H., Han, J., Lindgren, W., Macandrew, T., & English, A. H. (2012). Diagnostic considerations for novel influenza A (H1N1). International journal of pharmacy and pharmaceutical sciences, 4 (Suppl.1), 73-75. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.ijppsjournal.com/Vol4Suppl1/2931.pdf |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | International Journal of Pharmacy and Pharmaceutical Sciences |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Diagnostic considerations for Novel Influenza A (H1N1) |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Background: The emergence of novel swine-origin influenza A (H1N1) virus (S-OIV) infection represents a significant pandemic threat. Rapid and accurate diagnosis will enhance treatment and containment efforts. Methods: Ninety eight patients with influenza-like illness meeting the CDC’s guidelines for screening were tested with the rapid influenza test. Positive samples for influenza A were tested with real-time PCR (RT-PCR). Subsequently, all samples were tested using Target Enriched Multiplex Polymerase Chain Reaction (Tem-PCR). Results: Rapid influenza tests Conclusion: Clinical judgment should be applied when interpreting the results of the available S-OIV tests. A confirmatory and validated test for SOIV is urgently needed. confirmed 30 influenza A cases, 1 of influenza B, and resulted 67 negatives. The 30 samples positive for influenza A were tested using RT-PCR assay, which revealed 2 cases of seasonal influenza A, 7 cases of S-OIV, and 21 negatives for influenza infection. The TemPCR confirmed 1 of the seasonal influenza A cases but found the second case to be S-OIV. Tem-PCR confirmed the findings of RT-PCR in 3 of the SOIV cases but reported 4 of the cases negative for influenza. Additionally, Tem-PCR found 3 of the cases that were negative per RT-PCR were positive for S-OIV. The remainders were confirmed negative for influenza yet one was positive for adenovirus. The lone case of influenza B was confirmed with Tem-PCR. Among the 67 cases that were negative based on rapid influenza tests, Tem-PCR confirmed |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_cdc14b75693c1eb48400acbcf41c2514 |
| identifier_str_mv | Sheikh-Taha, M., Eiland E, Han J., Lindgren w., Macandrew English T., Hassoun A. (2012). Diagnostic considerations for novel influenza A (H1N1): International journal of pharmacy and pharmaceutical sciences,4 Suppl.1,73-75 0975-1491 Sheikh-Taha, M., Eiland III, E. H., Han, J., Lindgren, W., Macandrew, T., & English, A. H. (2012). Diagnostic considerations for novel influenza A (H1N1). International journal of pharmacy and pharmaceutical sciences, 4 (Suppl.1), 73-75. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/3937 |
| publishDate | 2012 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Diagnostic considerations for Novel Influenza A (H1N1)Sheikh-Taha, MarwanEiland III, Edward H.Han, JianLindgren, WillliamMacandrew, ThomasEnglish, Ali HassounBackground: The emergence of novel swine-origin influenza A (H1N1) virus (S-OIV) infection represents a significant pandemic threat. Rapid and accurate diagnosis will enhance treatment and containment efforts. Methods: Ninety eight patients with influenza-like illness meeting the CDC’s guidelines for screening were tested with the rapid influenza test. Positive samples for influenza A were tested with real-time PCR (RT-PCR). Subsequently, all samples were tested using Target Enriched Multiplex Polymerase Chain Reaction (Tem-PCR). Results: Rapid influenza tests Conclusion: Clinical judgment should be applied when interpreting the results of the available S-OIV tests. A confirmatory and validated test for SOIV is urgently needed. confirmed 30 influenza A cases, 1 of influenza B, and resulted 67 negatives. The 30 samples positive for influenza A were tested using RT-PCR assay, which revealed 2 cases of seasonal influenza A, 7 cases of S-OIV, and 21 negatives for influenza infection. The TemPCR confirmed 1 of the seasonal influenza A cases but found the second case to be S-OIV. Tem-PCR confirmed the findings of RT-PCR in 3 of the SOIV cases but reported 4 of the cases negative for influenza. Additionally, Tem-PCR found 3 of the cases that were negative per RT-PCR were positive for S-OIV. The remainders were confirmed negative for influenza yet one was positive for adenovirus. The lone case of influenza B was confirmed with Tem-PCR. Among the 67 cases that were negative based on rapid influenza tests, Tem-PCR confirmedPublishedN/A2016-06-02T13:08:34Z2016-06-02T13:08:34Z20122016-06-02Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSheikh-Taha, M., Eiland E, Han J., Lindgren w., Macandrew English T., Hassoun A. (2012). Diagnostic considerations for novel influenza A (H1N1): International journal of pharmacy and pharmaceutical sciences,4 Suppl.1,73-750975-1491http://hdl.handle.net/10725/3937Sheikh-Taha, M., Eiland III, E. H., Han, J., Lindgren, W., Macandrew, T., & English, A. H. (2012). Diagnostic considerations for novel influenza A (H1N1). International journal of pharmacy and pharmaceutical sciences, 4 (Suppl.1), 73-75.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://www.ijppsjournal.com/Vol4Suppl1/2931.pdfenInternational Journal of Pharmacy and Pharmaceutical Sciencesinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/39372021-03-19T10:00:50Z |
| spellingShingle | Diagnostic considerations for Novel Influenza A (H1N1) Sheikh-Taha, Marwan |
| status_str | publishedVersion |
| title | Diagnostic considerations for Novel Influenza A (H1N1) |
| title_full | Diagnostic considerations for Novel Influenza A (H1N1) |
| title_fullStr | Diagnostic considerations for Novel Influenza A (H1N1) |
| title_full_unstemmed | Diagnostic considerations for Novel Influenza A (H1N1) |
| title_short | Diagnostic considerations for Novel Influenza A (H1N1) |
| title_sort | Diagnostic considerations for Novel Influenza A (H1N1) |
| url | http://hdl.handle.net/10725/3937 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.ijppsjournal.com/Vol4Suppl1/2931.pdf |