The reductive enzymatic cleavage of thiosulfate
Methods presently used for the enzymatic assay of the thiosulfate cleaving reaction in bacterial cell-free systems are critically examined. Conditions under which strong acids are used to terminate the reaction and to release H2S proved to be unsuitable. A non-enzymatic production of H2S under such...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | |
| التنسيق: | article |
| منشور في: |
1972
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/3393 http://dx.doi.org/10.1007/BF00715022 http://link.springer.com/article/10.1007/BF00715022 |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513474001895424 |
|---|---|
| author | Hashwa, Fuad |
| author2 | Pfennig, Norbert |
| author2_role | author |
| author_facet | Hashwa, Fuad Pfennig, Norbert |
| author_role | author |
| dc.creator.none.fl_str_mv | Hashwa, Fuad Pfennig, Norbert |
| dc.date.none.fl_str_mv | 1972 2016-03-23T10:32:55Z 2016-03-23T10:32:55Z 2016-03-23 |
| dc.identifier.none.fl_str_mv | 0003-9276 http://hdl.handle.net/10725/3393 http://dx.doi.org/10.1007/BF00715022 Hashwa, F., & Pfennig, N. (1972). The reductive enzymatic cleavage of thiosulfate. Archiv für Mikrobiologie, 81(1), 36-44. http://link.springer.com/article/10.1007/BF00715022 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Archiv für Mikrobiologie |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | The reductive enzymatic cleavage of thiosulfate |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Methods presently used for the enzymatic assay of the thiosulfate cleaving reaction in bacterial cell-free systems are critically examined. Conditions under which strong acids are used to terminate the reaction and to release H2S proved to be unsuitable. A non-enzymatic production of H2S under such conditions is demonstrated. A reliable procedure for the measurement of H2S production from the enzymatic cleavage of thiosulfate is described. This method was used to measure the thiosulfate cleaving reaction catalyzed by cell-free extracts of phototrophic bacteria of the genusChromatium. As reductants, hydrogen-hydrogenase/methyl viologen system, reduced glutathione or dihydrolipoate were used. The same extract fraction catalyzed the rhodanese reaction. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_d037d53cd6ec8f8e1cdeec69108934b2 |
| identifier_str_mv | 0003-9276 Hashwa, F., & Pfennig, N. (1972). The reductive enzymatic cleavage of thiosulfate. Archiv für Mikrobiologie, 81(1), 36-44. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/3393 |
| publishDate | 1972 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | The reductive enzymatic cleavage of thiosulfateHashwa, FuadPfennig, NorbertMethods presently used for the enzymatic assay of the thiosulfate cleaving reaction in bacterial cell-free systems are critically examined. Conditions under which strong acids are used to terminate the reaction and to release H2S proved to be unsuitable. A non-enzymatic production of H2S under such conditions is demonstrated. A reliable procedure for the measurement of H2S production from the enzymatic cleavage of thiosulfate is described. This method was used to measure the thiosulfate cleaving reaction catalyzed by cell-free extracts of phototrophic bacteria of the genusChromatium. As reductants, hydrogen-hydrogenase/methyl viologen system, reduced glutathione or dihydrolipoate were used. The same extract fraction catalyzed the rhodanese reaction.PublishedN/A2016-03-23T10:32:55Z2016-03-23T10:32:55Z19722016-03-23Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0003-9276http://hdl.handle.net/10725/3393http://dx.doi.org/10.1007/BF00715022Hashwa, F., & Pfennig, N. (1972). The reductive enzymatic cleavage of thiosulfate. Archiv für Mikrobiologie, 81(1), 36-44.http://link.springer.com/article/10.1007/BF00715022enArchiv für Mikrobiologieinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/33932016-08-30T09:30:42Z |
| spellingShingle | The reductive enzymatic cleavage of thiosulfate Hashwa, Fuad |
| status_str | publishedVersion |
| title | The reductive enzymatic cleavage of thiosulfate |
| title_full | The reductive enzymatic cleavage of thiosulfate |
| title_fullStr | The reductive enzymatic cleavage of thiosulfate |
| title_full_unstemmed | The reductive enzymatic cleavage of thiosulfate |
| title_short | The reductive enzymatic cleavage of thiosulfate |
| title_sort | The reductive enzymatic cleavage of thiosulfate |
| url | http://hdl.handle.net/10725/3393 http://dx.doi.org/10.1007/BF00715022 http://link.springer.com/article/10.1007/BF00715022 |