Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon
Objectives The occurrence and dissemination of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare settings and the community and its risk of being introduced into hospitals are matters of great concern. The purpose of this study was to conduct a miniaturized epidemiological analysis of...
محفوظ في:
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| مؤلفون آخرون: | , |
| التنسيق: | article |
| منشور في: |
2014
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/3481 http://dx.doi.org/10.1016/j.ijid.2013.10.007 http://www.sciencedirect.com/science/article/pii/S1201971213003378 |
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إضافة وسم
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| _version_ | 1864513461307834368 |
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| author | Tokajian, Sima T. |
| author2 | Harastani, Houda H. Araj, George F. |
| author2_role | author author |
| author_facet | Tokajian, Sima T. Harastani, Houda H. Araj, George F. |
| author_role | author |
| dc.creator.none.fl_str_mv | Tokajian, Sima T. Harastani, Houda H. Araj, George F. |
| dc.date.none.fl_str_mv | 2014 2016-04-01T12:46:48Z 2016-04-01T12:46:48Z 2016-04-01 |
| dc.identifier.none.fl_str_mv | 1201-9712 http://hdl.handle.net/10725/3481 http://dx.doi.org/10.1016/j.ijid.2013.10.007 Harastani, H. H., Araj, G. F., & Tokajian, S. T. (2014). Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon. International Journal of Infectious Diseases, 19, 33-38. http://www.sciencedirect.com/science/article/pii/S1201971213003378 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | International Journal of Infectious Diseases |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Objectives The occurrence and dissemination of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare settings and the community and its risk of being introduced into hospitals are matters of great concern. The purpose of this study was to conduct a miniaturized epidemiological analysis of S. aureus-associated infections and to characterize the isolates by a variety of molecular typing techniques. Ongoing molecular surveillance is essential to prevent S. aureus strains from becoming endemic in the Lebanese healthcare setting. Methods A total of 132 S. aureus from different clinical specimens were isolated over a 6-month period. Characterization of the isolates was done by detection of the mecA gene, Panton–Valentine leukocidin determinant detection, staphylococcal chromosomal cassette (SCCmec) typing of MRSA, S. aureus protein A (spa) typing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and antibiogram analysis. Results MRSA represented 30% of the isolates, with PVL being detected in 54% of MRSA and 12% of methicillin-susceptible S. aureus (MSSA). A difference between MRSA and MSSA was observed in the spa types. Clustering SCCmec with MLST identified seven MRSA and 20 MSSA clones, with PVL-positive ST80-MRSA-IV being the dominant clone (7%), while PFGE revealed 32 groups with 80% cutoff similarity. Conclusions Although the results of this study are based on samples collected from one hospital, the high diversity observed along with the lack of any equivalence in the genetic backgrounds of the major MSSA and MRSA clones, emphasizes the urgent need for standardized surveillance combined with the application of well-validated typing methods to assess the occurrence of MRSA and subsequently to control its spread. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_d055991a814b8a13efe79e8e34cea93a |
| identifier_str_mv | 1201-9712 Harastani, H. H., Araj, G. F., & Tokajian, S. T. (2014). Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon. International Journal of Infectious Diseases, 19, 33-38. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/3481 |
| publishDate | 2014 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Molecular characteristics of Staphylococcus aureus isolated from a major hospital in LebanonTokajian, Sima T.Harastani, Houda H.Araj, George F.Objectives The occurrence and dissemination of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare settings and the community and its risk of being introduced into hospitals are matters of great concern. The purpose of this study was to conduct a miniaturized epidemiological analysis of S. aureus-associated infections and to characterize the isolates by a variety of molecular typing techniques. Ongoing molecular surveillance is essential to prevent S. aureus strains from becoming endemic in the Lebanese healthcare setting. Methods A total of 132 S. aureus from different clinical specimens were isolated over a 6-month period. Characterization of the isolates was done by detection of the mecA gene, Panton–Valentine leukocidin determinant detection, staphylococcal chromosomal cassette (SCCmec) typing of MRSA, S. aureus protein A (spa) typing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and antibiogram analysis. Results MRSA represented 30% of the isolates, with PVL being detected in 54% of MRSA and 12% of methicillin-susceptible S. aureus (MSSA). A difference between MRSA and MSSA was observed in the spa types. Clustering SCCmec with MLST identified seven MRSA and 20 MSSA clones, with PVL-positive ST80-MRSA-IV being the dominant clone (7%), while PFGE revealed 32 groups with 80% cutoff similarity. Conclusions Although the results of this study are based on samples collected from one hospital, the high diversity observed along with the lack of any equivalence in the genetic backgrounds of the major MSSA and MRSA clones, emphasizes the urgent need for standardized surveillance combined with the application of well-validated typing methods to assess the occurrence of MRSA and subsequently to control its spread.PublishedN/A2016-04-01T12:46:48Z2016-04-01T12:46:48Z20142016-04-01Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1201-9712http://hdl.handle.net/10725/3481http://dx.doi.org/10.1016/j.ijid.2013.10.007Harastani, H. H., Araj, G. F., & Tokajian, S. T. (2014). Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon. International Journal of Infectious Diseases, 19, 33-38.http://www.sciencedirect.com/science/article/pii/S1201971213003378enInternational Journal of Infectious Diseasesinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/34812018-05-23T06:02:45Z |
| spellingShingle | Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon Tokajian, Sima T. |
| status_str | publishedVersion |
| title | Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon |
| title_full | Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon |
| title_fullStr | Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon |
| title_full_unstemmed | Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon |
| title_short | Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon |
| title_sort | Molecular characteristics of Staphylococcus aureus isolated from a major hospital in Lebanon |
| url | http://hdl.handle.net/10725/3481 http://dx.doi.org/10.1016/j.ijid.2013.10.007 http://www.sciencedirect.com/science/article/pii/S1201971213003378 |