The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility
Malignant astrocytomas are highly invasive into adjacent and distant regions of the normal brain. Rho GTPases are small monomeric G proteins that play important roles in cytoskeleton rearrangement, cell motility, and tumor invasion. In the present study, we show that the knock down of StarD13, a GTP...
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| مؤلفون آخرون: | , , , , , , , , |
| التنسيق: | article |
| منشور في: |
2014
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/5197 http://dx.doi.org/10.1016/j.yexcr.2013.11.023 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/S0014482713005168 |
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| _version_ | 1864513465373163520 |
|---|---|
| author | Khalil, Bassem D. |
| author2 | Hanna, Samer Saykali, Bechara A. El-Sitt, Sally Nasrallah, Anita Marston, Daniel El-Sabban, Marwan Hahn, Klaus M. Symons, Marc El-Sibai, Mirvat |
| author2_role | author author author author author author author author author |
| author_facet | Khalil, Bassem D. Hanna, Samer Saykali, Bechara A. El-Sitt, Sally Nasrallah, Anita Marston, Daniel El-Sabban, Marwan Hahn, Klaus M. Symons, Marc El-Sibai, Mirvat |
| author_role | author |
| dc.creator.none.fl_str_mv | Khalil, Bassem D. Hanna, Samer Saykali, Bechara A. El-Sitt, Sally Nasrallah, Anita Marston, Daniel El-Sabban, Marwan Hahn, Klaus M. Symons, Marc El-Sibai, Mirvat |
| dc.date.none.fl_str_mv | 2014 2017-02-08T12:39:16Z 2017-02-08T12:39:16Z 2017-02-08 |
| dc.identifier.none.fl_str_mv | 0014-4827 http://hdl.handle.net/10725/5197 http://dx.doi.org/10.1016/j.yexcr.2013.11.023 Khalil, B. D., Hanna, S., Saykali, B. A., El-Sitt, S., Nasrallah, A., Marston, D., ... & El-Sibai, M. (2014). The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility. Experimental cell research, 321(2), 109-122. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/S0014482713005168 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Experimental Cell Research |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Malignant astrocytomas are highly invasive into adjacent and distant regions of the normal brain. Rho GTPases are small monomeric G proteins that play important roles in cytoskeleton rearrangement, cell motility, and tumor invasion. In the present study, we show that the knock down of StarD13, a GTPase activating protein (GAP) for RhoA and Cdc42, inhibits astrocytoma cell migration through modulating focal adhesion dynamics and cell adhesion. This effect is mediated by the resulting constitutive activation of RhoA and the subsequent indirect inhibition of Rac. Using Total Internal Reflection Fluorescence (TIRF)-based Förster Resonance Energy Transfer (FRET), we show that RhoA activity localizes with focal adhesions at the basal surface of astrocytoma cells. Moreover, the knock down of StarD13 inhibits the cycling of RhoA activation at the rear edge of cells, which makes them defective in retracting their tail. This study highlights the importance of the regulation of RhoA activity in focal adhesions of astrocytoma cells and establishes StarD13 as a GAP playing a major role in this process. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_d59d769b16f5ba5f91f98e665f76f554 |
| identifier_str_mv | 0014-4827 Khalil, B. D., Hanna, S., Saykali, B. A., El-Sitt, S., Nasrallah, A., Marston, D., ... & El-Sibai, M. (2014). The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility. Experimental cell research, 321(2), 109-122. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/5197 |
| publishDate | 2014 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motilityKhalil, Bassem D.Hanna, SamerSaykali, Bechara A.El-Sitt, SallyNasrallah, AnitaMarston, DanielEl-Sabban, MarwanHahn, Klaus M.Symons, MarcEl-Sibai, MirvatMalignant astrocytomas are highly invasive into adjacent and distant regions of the normal brain. Rho GTPases are small monomeric G proteins that play important roles in cytoskeleton rearrangement, cell motility, and tumor invasion. In the present study, we show that the knock down of StarD13, a GTPase activating protein (GAP) for RhoA and Cdc42, inhibits astrocytoma cell migration through modulating focal adhesion dynamics and cell adhesion. This effect is mediated by the resulting constitutive activation of RhoA and the subsequent indirect inhibition of Rac. Using Total Internal Reflection Fluorescence (TIRF)-based Förster Resonance Energy Transfer (FRET), we show that RhoA activity localizes with focal adhesions at the basal surface of astrocytoma cells. Moreover, the knock down of StarD13 inhibits the cycling of RhoA activation at the rear edge of cells, which makes them defective in retracting their tail. This study highlights the importance of the regulation of RhoA activity in focal adhesions of astrocytoma cells and establishes StarD13 as a GAP playing a major role in this process.PublishedN/A2017-02-08T12:39:16Z2017-02-08T12:39:16Z20142017-02-08Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0014-4827http://hdl.handle.net/10725/5197http://dx.doi.org/10.1016/j.yexcr.2013.11.023Khalil, B. D., Hanna, S., Saykali, B. A., El-Sitt, S., Nasrallah, A., Marston, D., ... & El-Sibai, M. (2014). The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility. Experimental cell research, 321(2), 109-122.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://www.sciencedirect.com/science/article/pii/S0014482713005168enExperimental Cell Researchinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/51972021-03-19T10:03:22Z |
| spellingShingle | The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility Khalil, Bassem D. |
| status_str | publishedVersion |
| title | The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility |
| title_full | The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility |
| title_fullStr | The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility |
| title_full_unstemmed | The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility |
| title_short | The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility |
| title_sort | The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility |
| url | http://hdl.handle.net/10725/5197 http://dx.doi.org/10.1016/j.yexcr.2013.11.023 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/S0014482713005168 |