Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater

Although membrane bioreactor (MBR) systems provide better removal of pathogens compared to conventional activated sludge processes, they do not achieve total log removal. The present study examines two MBR systems treating municipal wastewater, one a full-scale MBR plant and the other a lab-scale an...

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Main Author: Harb, Moustapha (author)
Other Authors: Hong, Pei-Ying (author)
Format: article
Published: 2017
Online Access:http://hdl.handle.net/10725/10152
http://dx.doi.org/10.1007/s11356-016-8211-y
http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php
https://link.springer.com/article/10.1007/s11356-016-8211-y
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author Harb, Moustapha
author2 Hong, Pei-Ying
author2_role author
author_facet Harb, Moustapha
Hong, Pei-Ying
author_role author
dc.creator.none.fl_str_mv Harb, Moustapha
Hong, Pei-Ying
dc.date.none.fl_str_mv 2017
2019-03-08T14:26:55Z
2019-03-08T14:26:55Z
2019-03-08
dc.identifier.none.fl_str_mv 1614-7499
http://hdl.handle.net/10725/10152
http://dx.doi.org/10.1007/s11356-016-8211-y
Harb, M., & Hong, P. Y. (2017). Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater: a case study. Environmental Science and Pollution Research, 24(6), 5370-5380.
http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php
https://link.springer.com/article/10.1007/s11356-016-8211-y
dc.language.none.fl_str_mv en
dc.relation.none.fl_str_mv Environmental Science and Pollution Research
dc.rights.*.fl_str_mv info:eu-repo/semantics/openAccess
dc.title.none.fl_str_mv Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater
a case study
dc.type.none.fl_str_mv Article
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
description Although membrane bioreactor (MBR) systems provide better removal of pathogens compared to conventional activated sludge processes, they do not achieve total log removal. The present study examines two MBR systems treating municipal wastewater, one a full-scale MBR plant and the other a lab-scale anaerobic MBR. Both of these systems were operated using microfiltration (MF) polymeric membranes. High-throughput sequencing and digital PCR quantification were utilized to monitor the log removal values (LRVs) of associated pathogenic species and their abundance in the MBR effluents. Results showed that specific removal rates vary widely regardless of the system employed. Each of the two MBR effluents’ microbial communities contained genera associated with opportunistic pathogens (e.g., Pseudomonas, Acinetobacter) with a wide range of log reduction values (< 2 to >5.5). Digital PCR further confirmed that these bacterial groups included pathogenic species, in several instances at LRVs different than those for their respective genera. These results were used to evaluate the potential risks associated both with the reuse of the MBR effluents for irrigation purposes and with land application of the activated sludge from the full-scale MBR system.
eu_rights_str_mv openAccess
format article
id LAURepo_d7802c164a699bc5503e0d82daaaba93
identifier_str_mv 1614-7499
Harb, M., & Hong, P. Y. (2017). Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater: a case study. Environmental Science and Pollution Research, 24(6), 5370-5380.
language_invalid_str_mv en
network_acronym_str LAURepo
network_name_str Lebanese American University repository
oai_identifier_str oai:laur.lau.edu.lb:10725/10152
publishDate 2017
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spelling Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewatera case studyHarb, MoustaphaHong, Pei-YingAlthough membrane bioreactor (MBR) systems provide better removal of pathogens compared to conventional activated sludge processes, they do not achieve total log removal. The present study examines two MBR systems treating municipal wastewater, one a full-scale MBR plant and the other a lab-scale anaerobic MBR. Both of these systems were operated using microfiltration (MF) polymeric membranes. High-throughput sequencing and digital PCR quantification were utilized to monitor the log removal values (LRVs) of associated pathogenic species and their abundance in the MBR effluents. Results showed that specific removal rates vary widely regardless of the system employed. Each of the two MBR effluents’ microbial communities contained genera associated with opportunistic pathogens (e.g., Pseudomonas, Acinetobacter) with a wide range of log reduction values (< 2 to >5.5). Digital PCR further confirmed that these bacterial groups included pathogenic species, in several instances at LRVs different than those for their respective genera. These results were used to evaluate the potential risks associated both with the reuse of the MBR effluents for irrigation purposes and with land application of the activated sludge from the full-scale MBR system.PublishedN/A2019-03-08T14:26:55Z2019-03-08T14:26:55Z20172019-03-08Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1614-7499http://hdl.handle.net/10725/10152http://dx.doi.org/10.1007/s11356-016-8211-yHarb, M., & Hong, P. Y. (2017). Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater: a case study. Environmental Science and Pollution Research, 24(6), 5370-5380.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://link.springer.com/article/10.1007/s11356-016-8211-yenEnvironmental Science and Pollution Researchinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/101522021-03-19T10:45:25Z
spellingShingle Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater
Harb, Moustapha
status_str publishedVersion
title Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater
title_full Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater
title_fullStr Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater
title_full_unstemmed Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater
title_short Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater
title_sort Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater
url http://hdl.handle.net/10725/10152
http://dx.doi.org/10.1007/s11356-016-8211-y
http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php
https://link.springer.com/article/10.1007/s11356-016-8211-y