Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence
We have previously characterized Pir32, a Candia albicans cell wall protein that we found to be involved in filamentation, virulence, chitin deposition, and resistance to oxidative stress. Other than defining the cell shape, the cell wall is critical for the interaction with the surrounding environm...
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2018
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| Online Access: | http://hdl.handle.net/10725/8457 https://doi.org/10.1371/journal.pone.0194403 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194403 |
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| _version_ | 1864513484957417472 |
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| author | El Khoury, Pamela |
| author2 | Awad, Andy Wex, Brigitte Khalaf, Roy A. |
| author2_role | author author author |
| author_facet | El Khoury, Pamela Awad, Andy Wex, Brigitte Khalaf, Roy A. |
| author_role | author |
| dc.creator.none.fl_str_mv | El Khoury, Pamela Awad, Andy Wex, Brigitte Khalaf, Roy A. |
| dc.date.none.fl_str_mv | 2018-09-13T10:48:16Z 2018-09-13T10:48:16Z 2018 2018-09-13 |
| dc.identifier.none.fl_str_mv | 1932-6203 http://hdl.handle.net/10725/8457 https://doi.org/10.1371/journal.pone.0194403 El Khoury, P., Awad, A., Wex, B., & Khalaf, R. A. (2018). Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence. PloS one, 13(3), 1-16. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194403 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Plos one |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | We have previously characterized Pir32, a Candia albicans cell wall protein that we found to be involved in filamentation, virulence, chitin deposition, and resistance to oxidative stress. Other than defining the cell shape, the cell wall is critical for the interaction with the surrounding environment and the point of contact and interaction with the host surface. In this study, we applied tandem mass spectrometry combined with bioinformatics to investigate cell wall proteome changes in a pir32 null strain. A total of 16 and 25 proteins were identified exclusively in the null mutant strains grown under non-filamentous and filamentous conditions. These proteins included members of the PGA family with various functions, lipase and the protease involved in virulence, superoxide dismutases required for resisting oxidative stress, alongside proteins required for cell wall remodeling and synthesis such as Ssr1, Xog1, Dfg5 and Dcw1. In addition proteins needed for filamentation like Cdc42, Ssu81 and Ucf1, and other virulence proteins such as Als3, Rbt5, and Csa2 were also detected. The detection of these proteins in the mutant and their lack of detection in the wild type can explain the differential phenotypes previously observed. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_e05140e380181f264421669b2be7ccdf |
| identifier_str_mv | 1932-6203 El Khoury, P., Awad, A., Wex, B., & Khalaf, R. A. (2018). Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence. PloS one, 13(3), 1-16. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/8457 |
| publishDate | 2018 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulenceEl Khoury, PamelaAwad, AndyWex, BrigitteKhalaf, Roy A.We have previously characterized Pir32, a Candia albicans cell wall protein that we found to be involved in filamentation, virulence, chitin deposition, and resistance to oxidative stress. Other than defining the cell shape, the cell wall is critical for the interaction with the surrounding environment and the point of contact and interaction with the host surface. In this study, we applied tandem mass spectrometry combined with bioinformatics to investigate cell wall proteome changes in a pir32 null strain. A total of 16 and 25 proteins were identified exclusively in the null mutant strains grown under non-filamentous and filamentous conditions. These proteins included members of the PGA family with various functions, lipase and the protease involved in virulence, superoxide dismutases required for resisting oxidative stress, alongside proteins required for cell wall remodeling and synthesis such as Ssr1, Xog1, Dfg5 and Dcw1. In addition proteins needed for filamentation like Cdc42, Ssu81 and Ucf1, and other virulence proteins such as Als3, Rbt5, and Csa2 were also detected. The detection of these proteins in the mutant and their lack of detection in the wild type can explain the differential phenotypes previously observed.PublishedN/A2018-09-13T10:48:16Z2018-09-13T10:48:16Z20182018-09-13Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1932-6203http://hdl.handle.net/10725/8457https://doi.org/10.1371/journal.pone.0194403El Khoury, P., Awad, A., Wex, B., & Khalaf, R. A. (2018). Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence. PloS one, 13(3), 1-16.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194403enPlos oneinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/84572021-03-19T10:45:17Z |
| spellingShingle | Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence El Khoury, Pamela |
| status_str | publishedVersion |
| title | Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence |
| title_full | Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence |
| title_fullStr | Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence |
| title_full_unstemmed | Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence |
| title_short | Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence |
| title_sort | Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence |
| url | http://hdl.handle.net/10725/8457 https://doi.org/10.1371/journal.pone.0194403 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194403 |