The Candida albicans Dse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and Virulence

The fungal pathogen Candida albicans is one of the leading causative agents of death in immunocompromised individuals. It harbors an arsenal of cell wall anchored factors that are implicated in virulence such as filamentation inducing factors, adhesins, lipases, proteases, and superoxide dismutases....

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Khalaf, Roy A. (author)
مؤلفون آخرون: Daher, Jalil Y. (author), Koussa, Joseph (author), Younes, Samer (author)
التنسيق: article
منشور في: 2011
الوصول للمادة أونلاين:http://hdl.handle.net/10725/3373
http://dx.doi.org/10.1155/2011/504280
http://www.hindawi.com/journals/ipid/2011/504280/abs/
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author Khalaf, Roy A.
author2 Daher, Jalil Y.
Koussa, Joseph
Younes, Samer
author2_role author
author
author
author_facet Khalaf, Roy A.
Daher, Jalil Y.
Koussa, Joseph
Younes, Samer
author_role author
dc.creator.none.fl_str_mv Khalaf, Roy A.
Daher, Jalil Y.
Koussa, Joseph
Younes, Samer
dc.date.none.fl_str_mv 2011
2016-03-21T09:28:51Z
2016-03-21T09:28:51Z
2016-03-21
dc.identifier.none.fl_str_mv 1687-708X
http://hdl.handle.net/10725/3373
http://dx.doi.org/10.1155/2011/504280
Daher, J. Y., Koussa, J., Younes, S., & Khalaf, R. A. (2011). The Candida albicans Dse1 protein is essential and plays a role in cell wall rigidity, biofilm formation, and virulence. Interdisciplinary perspectives on infectious diseases, 2011.
http://www.hindawi.com/journals/ipid/2011/504280/abs/
dc.language.none.fl_str_mv en
dc.relation.none.fl_str_mv Interdisciplinary Perspectives on Infectious Diseases
dc.rights.*.fl_str_mv info:eu-repo/semantics/openAccess
dc.title.none.fl_str_mv The Candida albicans Dse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and Virulence
dc.type.none.fl_str_mv Article
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
description The fungal pathogen Candida albicans is one of the leading causative agents of death in immunocompromised individuals. It harbors an arsenal of cell wall anchored factors that are implicated in virulence such as filamentation inducing factors, adhesins, lipases, proteases, and superoxide dismutases. Dse1 is a cell wall protein involved in cell wall metabolism. The purpose of this study is to characterize the role Dse1 plays in virulence. Dse1 appears to be an essential gene as no homozygous null mutant was possible. The heterozygote mutant exhibited increased susceptibility to calcofluor white, a cell wall disrupting agent, with a subsequent reduction in cell wall chitin content, decreased oxidative stress tolerance, a 30% reduction in biofilm formation, and a delay in adhesion that was mirrored by a reduction in virulence in a mouse model of infection. Dse1 thus appears to be an important protein involved in cell wall integrity and rigidity.
eu_rights_str_mv openAccess
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id LAURepo_e81a930821aa2e8a797df9037760e047
identifier_str_mv 1687-708X
Daher, J. Y., Koussa, J., Younes, S., & Khalaf, R. A. (2011). The Candida albicans Dse1 protein is essential and plays a role in cell wall rigidity, biofilm formation, and virulence. Interdisciplinary perspectives on infectious diseases, 2011.
language_invalid_str_mv en
network_acronym_str LAURepo
network_name_str Lebanese American University repository
oai_identifier_str oai:laur.lau.edu.lb:10725/3373
publishDate 2011
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spelling The Candida albicans Dse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and VirulenceKhalaf, Roy A.Daher, Jalil Y.Koussa, JosephYounes, SamerThe fungal pathogen Candida albicans is one of the leading causative agents of death in immunocompromised individuals. It harbors an arsenal of cell wall anchored factors that are implicated in virulence such as filamentation inducing factors, adhesins, lipases, proteases, and superoxide dismutases. Dse1 is a cell wall protein involved in cell wall metabolism. The purpose of this study is to characterize the role Dse1 plays in virulence. Dse1 appears to be an essential gene as no homozygous null mutant was possible. The heterozygote mutant exhibited increased susceptibility to calcofluor white, a cell wall disrupting agent, with a subsequent reduction in cell wall chitin content, decreased oxidative stress tolerance, a 30% reduction in biofilm formation, and a delay in adhesion that was mirrored by a reduction in virulence in a mouse model of infection. Dse1 thus appears to be an important protein involved in cell wall integrity and rigidity.PublishedN/A2016-03-21T09:28:51Z2016-03-21T09:28:51Z20112016-03-21Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1687-708Xhttp://hdl.handle.net/10725/3373http://dx.doi.org/10.1155/2011/504280Daher, J. Y., Koussa, J., Younes, S., & Khalaf, R. A. (2011). The Candida albicans Dse1 protein is essential and plays a role in cell wall rigidity, biofilm formation, and virulence. Interdisciplinary perspectives on infectious diseases, 2011.http://www.hindawi.com/journals/ipid/2011/504280/abs/enInterdisciplinary Perspectives on Infectious Diseasesinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/33732016-07-29T06:54:24Z
spellingShingle The Candida albicans Dse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and Virulence
Khalaf, Roy A.
status_str publishedVersion
title The Candida albicans Dse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and Virulence
title_full The Candida albicans Dse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and Virulence
title_fullStr The Candida albicans Dse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and Virulence
title_full_unstemmed The Candida albicans Dse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and Virulence
title_short The Candida albicans Dse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and Virulence
title_sort The Candida albicans Dse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and Virulence
url http://hdl.handle.net/10725/3373
http://dx.doi.org/10.1155/2011/504280
http://www.hindawi.com/journals/ipid/2011/504280/abs/