A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds
Exposure to vapours of volatile chemicals is a major occupational and environmental health concern. Toxicity testing of volatile organic compounds (VOCs) has always faced significant technological problems due to their high volatility and/or low solubility. The aim of this study was to develop a pra...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2006
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/6325 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://pubs.rsc.org/-/content/articlehtml/2006/em/b509812b |
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| _version_ | 1864513478998360064 |
|---|---|
| author | Khalil, C. |
| author2 | Hayes, A. Bakand, S. Winder, C. |
| author2_role | author author author |
| author_facet | Khalil, C. Hayes, A. Bakand, S. Winder, C. |
| author_role | author |
| dc.creator.none.fl_str_mv | Khalil, C. Hayes, A. Bakand, S. Winder, C. |
| dc.date.none.fl_str_mv | 2006 2017-10-17T09:25:27Z 2017-10-17T09:25:27Z 2017-10-17 |
| dc.identifier.none.fl_str_mv | 1464-0333 http://hdl.handle.net/10725/6325 Bakand, S., Winder, C., Khalil, C., & Hayes, A. (2006). A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds. Journal of Environmental Monitoring, 8(1), 100-105. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://pubs.rsc.org/-/content/articlehtml/2006/em/b509812b |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of Environmental Monitoring |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Exposure to vapours of volatile chemicals is a major occupational and environmental health concern. Toxicity testing of volatile organic compounds (VOCs) has always faced significant technological problems due to their high volatility and/or low solubility. The aim of this study was to develop a practical and reproducible in vitro exposure technique for toxicity testing of VOCs. Standard test atmospheres of xylene and toluene were generated in glass chambers using a static method. Human cells including: A549-lung derived cell lines, HepG2-liver derived cell lines and skin fibroblasts, were grown in porous membranes and exposed to various airborne concentrations of selected VOCs directly at the air/liquid interface for 1 h at 37 °C. Cytotoxicity of test chemicals was investigated using the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) and NRU (neutral red uptake) assays following 24 h incubation. Airborne IC50 (50% inhibitory concentration) values were determined using dose response curves for xylene (IC50 = 5350 ± 328 ppm, NRU; IC50 = 5750 ± 433 ppm, MTS in skin fibroblast) and toluene (IC50 = 10[thin space (1/6-em)]500 ± 527 ppm, NRU; IC50 = 11[thin space (1/6-em)]200 ± 1044 ppm, MTS in skin fibroblast). Our findings suggest that static direct exposure at the air/liquid interface is a practical and reproducible technique for toxicity testing of VOCs. Further, this technique can be used for inhalational and dermal toxicity studies of volatile chemicals in vitro as the exposure pattern in vivo is closely simulated by this method. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_ef8f84a011e23b22e7573e1e07671e33 |
| identifier_str_mv | 1464-0333 Bakand, S., Winder, C., Khalil, C., & Hayes, A. (2006). A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds. Journal of Environmental Monitoring, 8(1), 100-105. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6325 |
| publishDate | 2006 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | A novel in vitro exposure technique for toxicity testing of selected volatile organic compoundsKhalil, C.Hayes, A.Bakand, S.Winder, C.Exposure to vapours of volatile chemicals is a major occupational and environmental health concern. Toxicity testing of volatile organic compounds (VOCs) has always faced significant technological problems due to their high volatility and/or low solubility. The aim of this study was to develop a practical and reproducible in vitro exposure technique for toxicity testing of VOCs. Standard test atmospheres of xylene and toluene were generated in glass chambers using a static method. Human cells including: A549-lung derived cell lines, HepG2-liver derived cell lines and skin fibroblasts, were grown in porous membranes and exposed to various airborne concentrations of selected VOCs directly at the air/liquid interface for 1 h at 37 °C. Cytotoxicity of test chemicals was investigated using the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) and NRU (neutral red uptake) assays following 24 h incubation. Airborne IC50 (50% inhibitory concentration) values were determined using dose response curves for xylene (IC50 = 5350 ± 328 ppm, NRU; IC50 = 5750 ± 433 ppm, MTS in skin fibroblast) and toluene (IC50 = 10[thin space (1/6-em)]500 ± 527 ppm, NRU; IC50 = 11[thin space (1/6-em)]200 ± 1044 ppm, MTS in skin fibroblast). Our findings suggest that static direct exposure at the air/liquid interface is a practical and reproducible technique for toxicity testing of VOCs. Further, this technique can be used for inhalational and dermal toxicity studies of volatile chemicals in vitro as the exposure pattern in vivo is closely simulated by this method.PublishedN/A2017-10-17T09:25:27Z2017-10-17T09:25:27Z20062017-10-17Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1464-0333http://hdl.handle.net/10725/6325Bakand, S., Winder, C., Khalil, C., & Hayes, A. (2006). A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds. Journal of Environmental Monitoring, 8(1), 100-105.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://pubs.rsc.org/-/content/articlehtml/2006/em/b509812benJournal of Environmental Monitoringinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/63252021-03-19T10:03:25Z |
| spellingShingle | A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds Khalil, C. |
| status_str | publishedVersion |
| title | A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds |
| title_full | A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds |
| title_fullStr | A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds |
| title_full_unstemmed | A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds |
| title_short | A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds |
| title_sort | A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds |
| url | http://hdl.handle.net/10725/6325 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://pubs.rsc.org/-/content/articlehtml/2006/em/b509812b |