Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning
We describe step-by-step instructions to: 1) efficiently engineer intestinal organoids using magnetic nanoparticles for lenti- or retroviral transduction, and, 2) generate frozen sections from engineered organoids. This approach provides a powerful tool to efficiently alter gene expression in organo...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| التنسيق: | article |
| منشور في: |
2019
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/11323 https://doi.org/10.3791/57040 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.jove.com/video/57040/genetic-engineering-primary-mouse-intestinal-organoids-using-magnetic |
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| _version_ | 1864513488655745024 |
|---|---|
| author | Xian, Lingling |
| author2 | Chia, Lionel Georgess, Dan Shuai, Shuai Ewald, Andrew J. Resar, Linda M.S. |
| author2_role | author author author author author |
| author_facet | Xian, Lingling Chia, Lionel Georgess, Dan Shuai, Shuai Ewald, Andrew J. Resar, Linda M.S. |
| author_role | author |
| dc.creator.none.fl_str_mv | Xian, Lingling Chia, Lionel Georgess, Dan Shuai, Shuai Ewald, Andrew J. Resar, Linda M.S. |
| dc.date.none.fl_str_mv | 2019-09-20T12:14:29Z 2019-09-20T12:14:29Z 2019 2019-09-20 |
| dc.identifier.none.fl_str_mv | 1940-087X http://hdl.handle.net/10725/11323 https://doi.org/10.3791/57040 Xian, L., Chia, L., Georgess, D., Luo, L., Shuai, S., Ewald, A. J., & Resar, L. M. (2019). Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning. JoVE (Journal of Visualized Experiments), (147), e57040. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.jove.com/video/57040/genetic-engineering-primary-mouse-intestinal-organoids-using-magnetic |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of Visualized Experiments |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | We describe step-by-step instructions to: 1) efficiently engineer intestinal organoids using magnetic nanoparticles for lenti- or retroviral transduction, and, 2) generate frozen sections from engineered organoids. This approach provides a powerful tool to efficiently alter gene expression in organoids for investigation of downstream effects. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_f675bd836fb4fe773b5b6d7b00b14809 |
| identifier_str_mv | 1940-087X Xian, L., Chia, L., Georgess, D., Luo, L., Shuai, S., Ewald, A. J., & Resar, L. M. (2019). Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning. JoVE (Journal of Visualized Experiments), (147), e57040. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/11323 |
| publishDate | 2019 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioningXian, LinglingChia, LionelGeorgess, DanShuai, ShuaiEwald, Andrew J.Resar, Linda M.S.We describe step-by-step instructions to: 1) efficiently engineer intestinal organoids using magnetic nanoparticles for lenti- or retroviral transduction, and, 2) generate frozen sections from engineered organoids. This approach provides a powerful tool to efficiently alter gene expression in organoids for investigation of downstream effects.PublishedN/A2019-09-20T12:14:29Z2019-09-20T12:14:29Z20192019-09-20Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1940-087Xhttp://hdl.handle.net/10725/11323https://doi.org/10.3791/57040Xian, L., Chia, L., Georgess, D., Luo, L., Shuai, S., Ewald, A. J., & Resar, L. M. (2019). Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning. JoVE (Journal of Visualized Experiments), (147), e57040.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.jove.com/video/57040/genetic-engineering-primary-mouse-intestinal-organoids-using-magneticenJournal of Visualized Experimentsinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/113232021-03-19T10:47:36Z |
| spellingShingle | Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning Xian, Lingling |
| status_str | publishedVersion |
| title | Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning |
| title_full | Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning |
| title_fullStr | Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning |
| title_full_unstemmed | Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning |
| title_short | Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning |
| title_sort | Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning |
| url | http://hdl.handle.net/10725/11323 https://doi.org/10.3791/57040 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.jove.com/video/57040/genetic-engineering-primary-mouse-intestinal-organoids-using-magnetic |