Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning
We describe step-by-step instructions to: 1) efficiently engineer intestinal organoids using magnetic nanoparticles for lenti- or retroviral transduction, and, 2) generate frozen sections from engineered organoids. This approach provides a powerful tool to efficiently alter gene expression in organo...
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| Other Authors: | , , , , |
| Format: | article |
| Published: |
2019
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| Online Access: | http://hdl.handle.net/10725/11323 https://doi.org/10.3791/57040 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php https://www.jove.com/video/57040/genetic-engineering-primary-mouse-intestinal-organoids-using-magnetic |
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