High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations
Background Liquid chromatography–tandem mass spectrometry (LC–MS/MS) is widely used for the measurement of 25-hydroxyvitamin D3 (25OHD3) and 25-hydroxyvitamin D2 (25OHD2) in blood. However, the presence of 25OHD3 C3-epimer (3-epi-25OHD3) may cause interference and overestimation of the 25OHD3 level....
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| التنسيق: | article |
| منشور في: |
2016
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/4031 http://dx.doi.org/10.1016/j.cca.2016.01.004 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/S000989811630002X |
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| _version_ | 1864513462821978112 |
|---|---|
| author | El-Khoury, Joe |
| author2 | Yang, Yifei Rogers, Katherine Wardle, Robert |
| author2_role | author author author |
| author_facet | El-Khoury, Joe Yang, Yifei Rogers, Katherine Wardle, Robert |
| author_role | author |
| dc.creator.none.fl_str_mv | El-Khoury, Joe Yang, Yifei Rogers, Katherine Wardle, Robert |
| dc.date.none.fl_str_mv | 2016-06-14T09:17:43Z 2016-06-14T09:17:43Z 2016 2016-06-14 |
| dc.identifier.none.fl_str_mv | 0009-8981 http://hdl.handle.net/10725/4031 http://dx.doi.org/10.1016/j.cca.2016.01.004 Yang, Y., Rogers, K., Wardle, R., & El-Khoury, J. M. (2016). High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations. Clinica Chimica Acta, 454, 102-106. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/S000989811630002X |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Clinica Chimica Acta |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Background Liquid chromatography–tandem mass spectrometry (LC–MS/MS) is widely used for the measurement of 25-hydroxyvitamin D3 (25OHD3) and 25-hydroxyvitamin D2 (25OHD2) in blood. However, the presence of 25OHD3 C3-epimer (3-epi-25OHD3) may cause interference and overestimation of the 25OHD3 level. We developed a rapid and simple assay for measurement of 25OHD that separates this interference and to investigate its impact on 25OHD3 measurement in adult and pediatric populations. Methods Sample preparation consisted of protein precipitation followed by solid-phase extraction with an LC run time of 4.8 min. Method comparison with another LC–MS/MS method of a major reference laboratory that does not separate the C3-epimer interference was performed using adult (n = 52) and pediatric (n = 40) samples. Results This method is free from significant ion suppression, carryover and interference. The assay can separate 25OHD3 from the 3-epi-25OHD3, and can measure 25OHD2 and 25OHD3 from 4.2 to 310.7 ng/ml and 5.2 to 311.1 ng/ml, respectively. Method comparison with a LC–MS method that does not separate the interference revealed biases of − 0.15 and 4.54 for 25OHD3 measurement in adult and pediatric samples, respectively. Conclusion A fast and simple LC–MS/MS method for quantification of 25OHD3 and 25OHD2 without 3-epi-25OHD3 interference was developed. This assay is required for accurate quantitation of 25OHD3 in pediatric samples and suitable for routine use in a high volume clinical laboratory. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_f8e7913bf8b3e0f96743ab2c2fd09a6e |
| identifier_str_mv | 0009-8981 Yang, Y., Rogers, K., Wardle, R., & El-Khoury, J. M. (2016). High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations. Clinica Chimica Acta, 454, 102-106. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/4031 |
| publishDate | 2016 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populationsEl-Khoury, JoeYang, YifeiRogers, KatherineWardle, RobertBackground Liquid chromatography–tandem mass spectrometry (LC–MS/MS) is widely used for the measurement of 25-hydroxyvitamin D3 (25OHD3) and 25-hydroxyvitamin D2 (25OHD2) in blood. However, the presence of 25OHD3 C3-epimer (3-epi-25OHD3) may cause interference and overestimation of the 25OHD3 level. We developed a rapid and simple assay for measurement of 25OHD that separates this interference and to investigate its impact on 25OHD3 measurement in adult and pediatric populations. Methods Sample preparation consisted of protein precipitation followed by solid-phase extraction with an LC run time of 4.8 min. Method comparison with another LC–MS/MS method of a major reference laboratory that does not separate the C3-epimer interference was performed using adult (n = 52) and pediatric (n = 40) samples. Results This method is free from significant ion suppression, carryover and interference. The assay can separate 25OHD3 from the 3-epi-25OHD3, and can measure 25OHD2 and 25OHD3 from 4.2 to 310.7 ng/ml and 5.2 to 311.1 ng/ml, respectively. Method comparison with a LC–MS method that does not separate the interference revealed biases of − 0.15 and 4.54 for 25OHD3 measurement in adult and pediatric samples, respectively. Conclusion A fast and simple LC–MS/MS method for quantification of 25OHD3 and 25OHD2 without 3-epi-25OHD3 interference was developed. This assay is required for accurate quantitation of 25OHD3 in pediatric samples and suitable for routine use in a high volume clinical laboratory.PublishedN/A2016-06-14T09:17:43Z2016-06-14T09:17:43Z20162016-06-14Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0009-8981http://hdl.handle.net/10725/4031http://dx.doi.org/10.1016/j.cca.2016.01.004Yang, Y., Rogers, K., Wardle, R., & El-Khoury, J. M. (2016). High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations. Clinica Chimica Acta, 454, 102-106.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://www.sciencedirect.com/science/article/pii/S000989811630002XenClinica Chimica Actainfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/40312021-03-19T10:00:50Z |
| spellingShingle | High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations El-Khoury, Joe |
| status_str | publishedVersion |
| title | High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations |
| title_full | High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations |
| title_fullStr | High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations |
| title_full_unstemmed | High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations |
| title_short | High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations |
| title_sort | High-throughput measurement of 25-hydroxyvitamin D by LC–MS/MS with separation of the C3-epimer interference for pediatric populations |
| url | http://hdl.handle.net/10725/4031 http://dx.doi.org/10.1016/j.cca.2016.01.004 http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://www.sciencedirect.com/science/article/pii/S000989811630002X |