Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains

We studied 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neurotoxicity in 5 strains of rats by assessing mortality and brain monoamine changes after MPTP injections into the internal carotid artery. We then attempted to correlate the differences among rat strains in their susceptibility to MPT...

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Main Author: Riachi, Naji J. (author)
Other Authors: Behmand, Ramin A. (author), Harik, Sami I. (author)
Format: article
Published: 1991
Online Access:http://hdl.handle.net/10725/10433
https://doi.org/10.1016/0006-8993(91)90854-O
http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php
https://www.sciencedirect.com/science/article/pii/000689939190854O
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author Riachi, Naji J.
author2 Behmand, Ramin A.
Harik, Sami I.
author2_role author
author
author_facet Riachi, Naji J.
Behmand, Ramin A.
Harik, Sami I.
author_role author
dc.creator.none.fl_str_mv Riachi, Naji J.
Behmand, Ramin A.
Harik, Sami I.
dc.date.none.fl_str_mv 1991
2019-04-15T09:50:31Z
2019-04-15T09:50:31Z
2019-04-15
dc.identifier.none.fl_str_mv 1872-6240
http://hdl.handle.net/10725/10433
https://doi.org/10.1016/0006-8993(91)90854-O
Riachi, N. J., Behmand, R. A., & Harik, S. I. (1991). Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains. Brain research, 555(1), 19-24.
http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php
https://www.sciencedirect.com/science/article/pii/000689939190854O
dc.language.none.fl_str_mv en
dc.relation.none.fl_str_mv Brain Research
dc.rights.*.fl_str_mv info:eu-repo/semantics/openAccess
dc.title.none.fl_str_mv Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains
dc.type.none.fl_str_mv Article
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
description We studied 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neurotoxicity in 5 strains of rats by assessing mortality and brain monoamine changes after MPTP injections into the internal carotid artery. We then attempted to correlate the differences among rat strains in their susceptibility to MPTP neurotoxicity in vivo with MPTP oxidation by monoamine oxidase (MAO) of the cerebral cortex, striatum, and brain microvessels in vitro. Despite the fact that the carotid route delivers much higher amounts of MPTP to the ipsilateral cerebrum than can be achieved by systemic injections, no significant dopamine depletion occured in ipsilateral striata of Sprague-Dawley rats (the most resistant strain), but significant reductions of about 40% in striatal dopamine were evident in the more sensitive strains. Decreased striatal dopamine levels in these latter rat strains were associated with increased dopamine turnover. Higher doses of MPTP resulted in acute death. MPTP-induced mortality was not affected, but striatal dopamine depletion was prevented, by MAO inhibition. Differences among rat strains in their susceptibility to MPTP neurotoxicity correlated best with MAO activity in their isolated brain microvessels, but not with MAO activity in their striata or cerebral cortices. These results are consistent with the hypothesis that the rats' resistance to MPTP neurotoxicity is to some extent a property of their unique brain endothelium which has high MAO activity.
eu_rights_str_mv openAccess
format article
id LAURepo_fc0b311ab074586e4d230bb7820a9ee8
identifier_str_mv 1872-6240
Riachi, N. J., Behmand, R. A., & Harik, S. I. (1991). Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains. Brain research, 555(1), 19-24.
language_invalid_str_mv en
network_acronym_str LAURepo
network_name_str Lebanese American University repository
oai_identifier_str oai:laur.lau.edu.lb:10725/10433
publishDate 1991
repository.mail.fl_str_mv
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spelling Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strainsRiachi, Naji J.Behmand, Ramin A.Harik, Sami I.We studied 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neurotoxicity in 5 strains of rats by assessing mortality and brain monoamine changes after MPTP injections into the internal carotid artery. We then attempted to correlate the differences among rat strains in their susceptibility to MPTP neurotoxicity in vivo with MPTP oxidation by monoamine oxidase (MAO) of the cerebral cortex, striatum, and brain microvessels in vitro. Despite the fact that the carotid route delivers much higher amounts of MPTP to the ipsilateral cerebrum than can be achieved by systemic injections, no significant dopamine depletion occured in ipsilateral striata of Sprague-Dawley rats (the most resistant strain), but significant reductions of about 40% in striatal dopamine were evident in the more sensitive strains. Decreased striatal dopamine levels in these latter rat strains were associated with increased dopamine turnover. Higher doses of MPTP resulted in acute death. MPTP-induced mortality was not affected, but striatal dopamine depletion was prevented, by MAO inhibition. Differences among rat strains in their susceptibility to MPTP neurotoxicity correlated best with MAO activity in their isolated brain microvessels, but not with MAO activity in their striata or cerebral cortices. These results are consistent with the hypothesis that the rats' resistance to MPTP neurotoxicity is to some extent a property of their unique brain endothelium which has high MAO activity.PublishedN/A2019-04-15T09:50:31Z2019-04-15T09:50:31Z19912019-04-15Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1872-6240http://hdl.handle.net/10725/10433https://doi.org/10.1016/0006-8993(91)90854-ORiachi, N. J., Behmand, R. A., & Harik, S. I. (1991). Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains. Brain research, 555(1), 19-24.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttps://www.sciencedirect.com/science/article/pii/000689939190854OenBrain Researchinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/104332021-03-19T10:45:29Z
spellingShingle Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains
Riachi, Naji J.
status_str publishedVersion
title Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains
title_full Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains
title_fullStr Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains
title_full_unstemmed Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains
title_short Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains
title_sort Correlation of MPTP neurotoxicity in vivo with oxidation of MPTP by the brain and blood-brain barrier in vitro in five rat strains
url http://hdl.handle.net/10725/10433
https://doi.org/10.1016/0006-8993(91)90854-O
http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php
https://www.sciencedirect.com/science/article/pii/000689939190854O