Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls
Membranes from etiolated pea epicotyls were fractionated by discontinuous sucrose-density-gradient centrifugation into endoplasmic reticulum, Golgi apparatus and plasma membrane. The Golgi apparatus was further fractionated on a shallow, continuous sucrose density gradient into Golgi subfractions of...
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1999
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| Online Access: | http://hdl.handle.net/10725/2343 http://dx.doi.org/10.1016/S0176-1617(99)80013-3 http://www.sciencedirect.com/science/article/pii/S0176161799800133 |
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| _version_ | 1864513457944002561 |
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| author | Rizk, Sandra |
| author2 | Baydoun, Elias Brett, Christopher |
| author2_role | author author |
| author_facet | Rizk, Sandra Baydoun, Elias Brett, Christopher |
| author_role | author |
| dc.creator.none.fl_str_mv | Rizk, Sandra Baydoun, Elias Brett, Christopher |
| dc.date.none.fl_str_mv | 1999 2015-10-26T13:21:10Z 2015-10-26T13:21:10Z 2015-10-26 |
| dc.identifier.none.fl_str_mv | 0176-1617 http://hdl.handle.net/10725/2343 http://dx.doi.org/10.1016/S0176-1617(99)80013-3 Baydoun, E. A. H., Rizk, S. E., & Brett, C. T. (1999). Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls. Journal of plant physiology, 155(2), 240-244. http://www.sciencedirect.com/science/article/pii/S0176161799800133 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of plant physiology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Membranes from etiolated pea epicotyls were fractionated by discontinuous sucrose-density-gradient centrifugation into endoplasmic reticulum, Golgi apparatus and plasma membrane. The Golgi apparatus was further fractionated on a shallow, continuous sucrose density gradient into Golgi subfractions of low, medium and high density. Methyltransferases forming both EDTA-soluble and EDTA-insoluble methylated polysaccharides were found to be concentrated in the Golgi apparatus, chiefly in the low and medium density Golgi membranes. The products were characterised by acid and alkaline hydrolysis and enzymic digestion. These tests indicated that the EDTA-soluble methyltransferase product consisted mainly of methyl-esterified pectin, probably rhamnogalacturonan l, while the EDTA-insoluble methyltransferase product was mostly 4-O-methylglucuronoxylan. It was concluded that both pectin methyltransferase and glucuronoxylan methyltransferase are located in the low and medium density Golgi membranes, which may correspond to the cis- and medial-Golgi cisternae identified by electron microscopy. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_fd696f68facaa3a7f7cd17ce64afd739 |
| identifier_str_mv | 0176-1617 Baydoun, E. A. H., Rizk, S. E., & Brett, C. T. (1999). Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls. Journal of plant physiology, 155(2), 240-244. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/2343 |
| publishDate | 1999 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea EpicotylsRizk, SandraBaydoun, EliasBrett, ChristopherMembranes from etiolated pea epicotyls were fractionated by discontinuous sucrose-density-gradient centrifugation into endoplasmic reticulum, Golgi apparatus and plasma membrane. The Golgi apparatus was further fractionated on a shallow, continuous sucrose density gradient into Golgi subfractions of low, medium and high density. Methyltransferases forming both EDTA-soluble and EDTA-insoluble methylated polysaccharides were found to be concentrated in the Golgi apparatus, chiefly in the low and medium density Golgi membranes. The products were characterised by acid and alkaline hydrolysis and enzymic digestion. These tests indicated that the EDTA-soluble methyltransferase product consisted mainly of methyl-esterified pectin, probably rhamnogalacturonan l, while the EDTA-insoluble methyltransferase product was mostly 4-O-methylglucuronoxylan. It was concluded that both pectin methyltransferase and glucuronoxylan methyltransferase are located in the low and medium density Golgi membranes, which may correspond to the cis- and medial-Golgi cisternae identified by electron microscopy.PublishedN/A2015-10-26T13:21:10Z2015-10-26T13:21:10Z19992015-10-26Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0176-1617http://hdl.handle.net/10725/2343http://dx.doi.org/10.1016/S0176-1617(99)80013-3Baydoun, E. A. H., Rizk, S. E., & Brett, C. T. (1999). Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls. Journal of plant physiology, 155(2), 240-244.http://www.sciencedirect.com/science/article/pii/S0176161799800133enJournal of plant physiologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/23432016-07-22T08:11:14Z |
| spellingShingle | Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls Rizk, Sandra |
| status_str | publishedVersion |
| title | Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls |
| title_full | Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls |
| title_fullStr | Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls |
| title_full_unstemmed | Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls |
| title_short | Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls |
| title_sort | Localisation of Meth Itransferases Involved in Glucuronoxylan and Pectin Meth lation in the Golgi Apparatus in Etiolated Pea Epicotyls |
| url | http://hdl.handle.net/10725/2343 http://dx.doi.org/10.1016/S0176-1617(99)80013-3 http://www.sciencedirect.com/science/article/pii/S0176161799800133 |