In vivo real-time confocal reflectance microscopy
Background Mohs micrographic surgery (MMS) is based on microscopically controlled excision of cutaneous neoplasms and offers the highest cure rates with maximum tissue preservation. In vivo confocal microscopy (CM) allows noninvasive optical imaging of thin sections of living skin, in its native sta...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , |
| التنسيق: | article |
| منشور في: |
2003
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10725/6542 http://dx.doi.org/10.1046/j.1524-4725.2003.29219.x http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1046/j.1524-4725.2003.29219.x/full |
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| _version_ | 1864513480205271040 |
|---|---|
| author | Tannous, Zeina |
| author2 | Torres, Abel Gonzalez, Salvador |
| author2_role | author author |
| author_facet | Tannous, Zeina Torres, Abel Gonzalez, Salvador |
| author_role | author |
| dc.creator.none.fl_str_mv | Tannous, Zeina Torres, Abel Gonzalez, Salvador |
| dc.date.none.fl_str_mv | 2003 2017-11-08T09:15:23Z 2017-11-08T09:15:23Z 2017-11-08 |
| dc.identifier.none.fl_str_mv | 1524-4725 http://hdl.handle.net/10725/6542 http://dx.doi.org/10.1046/j.1524-4725.2003.29219.x Tannous, Z., Torres, A., & González, S. (2003). In Vivo Real‐Time Confocal Reflectance Microscopy: A Noninvasive Guide for Mohs Micrographic Surgery Facilitated by Aluminum Chloride, an Excellent Contrast Enhancer. Dermatologic surgery, 29(8), 839-846. http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1046/j.1524-4725.2003.29219.x/full |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Dermatologic Surgery |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | In vivo real-time confocal reflectance microscopy A noninvasive guide for mohs micrographic surgery facilitated by aluminum chloride, an excellent contrast enhancer |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Background Mohs micrographic surgery (MMS) is based on microscopically controlled excision of cutaneous neoplasms and offers the highest cure rates with maximum tissue preservation. In vivo confocal microscopy (CM) allows noninvasive optical imaging of thin sections of living skin, in its native state, in real time, with high resolution and contrast. Objective To evaluate the feasibility of the use of in vivo CM as a surgical guide in MMS. Methods Five patients with a biopsy-proven basal cell carcinoma (BCC) were imaged by in vivo CM on one or two sites from the clinically visible skin cancer. The first Mohs layer was then excised, and the fresh-frozen sections were correlated with the CM findings. Aluminum chloride (AlCl) 20% was applied on the Mohs defect followed by in vivo CM on one site from each lesion. A second Mohs layer was subsequently excised, and fresh-frozen sections were correlated with CM findings. Results The findings of in vivo CM were confirmed by hematoxylin and eosin–stained frozen sections after excisions of the first and second Mohs layers. AlCl was found to provide an excellent contrast between BCC cells and the surrounding tissue, detected readily with both in vivo and ex vivo CM. The tumor cells with AlCl exhibited intensely bright nuclei with an excellent contrast as compared with the low-contrast dark nuclei without AlCl application. Conclusion In vivo CM is a potential surgical guide for MMS, and AlCl provides an excellent exogenous agent to enhance tumor contrast for CM. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_fefe2f726b6a5e9010470ea3dc4dcbdc |
| identifier_str_mv | 1524-4725 Tannous, Z., Torres, A., & González, S. (2003). In Vivo Real‐Time Confocal Reflectance Microscopy: A Noninvasive Guide for Mohs Micrographic Surgery Facilitated by Aluminum Chloride, an Excellent Contrast Enhancer. Dermatologic surgery, 29(8), 839-846. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/6542 |
| publishDate | 2003 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | In vivo real-time confocal reflectance microscopyA noninvasive guide for mohs micrographic surgery facilitated by aluminum chloride, an excellent contrast enhancerTannous, ZeinaTorres, AbelGonzalez, SalvadorBackground Mohs micrographic surgery (MMS) is based on microscopically controlled excision of cutaneous neoplasms and offers the highest cure rates with maximum tissue preservation. In vivo confocal microscopy (CM) allows noninvasive optical imaging of thin sections of living skin, in its native state, in real time, with high resolution and contrast. Objective To evaluate the feasibility of the use of in vivo CM as a surgical guide in MMS. Methods Five patients with a biopsy-proven basal cell carcinoma (BCC) were imaged by in vivo CM on one or two sites from the clinically visible skin cancer. The first Mohs layer was then excised, and the fresh-frozen sections were correlated with the CM findings. Aluminum chloride (AlCl) 20% was applied on the Mohs defect followed by in vivo CM on one site from each lesion. A second Mohs layer was subsequently excised, and fresh-frozen sections were correlated with CM findings. Results The findings of in vivo CM were confirmed by hematoxylin and eosin–stained frozen sections after excisions of the first and second Mohs layers. AlCl was found to provide an excellent contrast between BCC cells and the surrounding tissue, detected readily with both in vivo and ex vivo CM. The tumor cells with AlCl exhibited intensely bright nuclei with an excellent contrast as compared with the low-contrast dark nuclei without AlCl application. Conclusion In vivo CM is a potential surgical guide for MMS, and AlCl provides an excellent exogenous agent to enhance tumor contrast for CM.PublishedN/A2017-11-08T09:15:23Z2017-11-08T09:15:23Z20032017-11-08Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1524-4725http://hdl.handle.net/10725/6542http://dx.doi.org/10.1046/j.1524-4725.2003.29219.xTannous, Z., Torres, A., & González, S. (2003). In Vivo Real‐Time Confocal Reflectance Microscopy: A Noninvasive Guide for Mohs Micrographic Surgery Facilitated by Aluminum Chloride, an Excellent Contrast Enhancer. Dermatologic surgery, 29(8), 839-846.http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.phphttp://onlinelibrary.wiley.com/doi/10.1046/j.1524-4725.2003.29219.x/fullenDermatologic Surgeryinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/65422021-03-19T10:03:27Z |
| spellingShingle | In vivo real-time confocal reflectance microscopy Tannous, Zeina |
| status_str | publishedVersion |
| title | In vivo real-time confocal reflectance microscopy |
| title_full | In vivo real-time confocal reflectance microscopy |
| title_fullStr | In vivo real-time confocal reflectance microscopy |
| title_full_unstemmed | In vivo real-time confocal reflectance microscopy |
| title_short | In vivo real-time confocal reflectance microscopy |
| title_sort | In vivo real-time confocal reflectance microscopy |
| url | http://hdl.handle.net/10725/6542 http://dx.doi.org/10.1046/j.1524-4725.2003.29219.x http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php http://onlinelibrary.wiley.com/doi/10.1046/j.1524-4725.2003.29219.x/full |