Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon
Streptococcus pyogenes [Group A Streptococcus (GAS)] is one of the most important human pathogens, responsible for numerous diseases with diverse clinical manifestations. As the epidemiology of GAS infections evolves, a rapid and reliable characterization of the isolates remains essential for epidem...
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2014
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| Online Access: | http://hdl.handle.net/10725/3484 http://dx.doi.org/10.1099/jmm.0.063412-0 http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.063412-0 |
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| _version_ | 1864513461310980096 |
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| author | Tokajian, Sima T. |
| author2 | Karaky, Nathalie M. Araj, George F. |
| author2_role | author author |
| author_facet | Tokajian, Sima T. Karaky, Nathalie M. Araj, George F. |
| author_role | author |
| dc.creator.none.fl_str_mv | Tokajian, Sima T. Karaky, Nathalie M. Araj, George F. |
| dc.date.none.fl_str_mv | 2014 2016-04-05T09:06:25Z 2016-04-05T09:06:25Z 2016-04-05 |
| dc.identifier.none.fl_str_mv | 0022-2615 http://hdl.handle.net/10725/3484 http://dx.doi.org/10.1099/jmm.0.063412-0 Karaky, N. M., Araj, G. F., & Tokajian, S. T. (2014). Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon. Journal of medical microbiology, 63(9), 1197-1204. http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.063412-0 |
| dc.language.none.fl_str_mv | en |
| dc.relation.none.fl_str_mv | Journal of Medical Microbiology |
| dc.rights.*.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.title.none.fl_str_mv | Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon |
| dc.type.none.fl_str_mv | Article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Streptococcus pyogenes [Group A Streptococcus (GAS)] is one of the most important human pathogens, responsible for numerous diseases with diverse clinical manifestations. As the epidemiology of GAS infections evolves, a rapid and reliable characterization of the isolates remains essential for epidemiological analysis and infection control. This study investigated the epidemiological patterns and genetic characteristics of 150 GAS isolates from a tertiary hospital in Lebanon by emm typing, superantigens (SAgs) detection, PFGE and antibiotic profiling. The results revealed 41 distinct emm types, the most prevalent of which were emm89 (16 %), emm12 (10 %), emm2 (9 %) and emm1 (8 %). Testing for the presence of superantigens showed that speB (87 %), ssa (36 %) and speG (30 %) were predominant. PFGE detected 39 pulsotypes when a similarity cut-off value of 80 % was implemented. Antibiotic-susceptibility testing against seven different classes of antibiotics showed that 9 % of the isolates were resistant to clindamycin, 23 % were resistant to erythromycin and 4 % showed the macrolide–lincosamide–streptogramin B (MLSB) phenotype. The emergence of tetracycline-resistant strains (37 %) was high when compared with previous reports from Lebanon. This study provided comprehensive evidence of the epidemiology of GAS in Lebanon, highlighting the association between emm types and toxin genes, and providing valuable information about the origin and dissemination of this pathogen. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | LAURepo_ff69ea72f7df8a12bd43e36b9b5f6310 |
| identifier_str_mv | 0022-2615 Karaky, N. M., Araj, G. F., & Tokajian, S. T. (2014). Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon. Journal of medical microbiology, 63(9), 1197-1204. |
| language_invalid_str_mv | en |
| network_acronym_str | LAURepo |
| network_name_str | Lebanese American University repository |
| oai_identifier_str | oai:laur.lau.edu.lb:10725/3484 |
| publishDate | 2014 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in LebanonTokajian, Sima T.Karaky, Nathalie M.Araj, George F.Streptococcus pyogenes [Group A Streptococcus (GAS)] is one of the most important human pathogens, responsible for numerous diseases with diverse clinical manifestations. As the epidemiology of GAS infections evolves, a rapid and reliable characterization of the isolates remains essential for epidemiological analysis and infection control. This study investigated the epidemiological patterns and genetic characteristics of 150 GAS isolates from a tertiary hospital in Lebanon by emm typing, superantigens (SAgs) detection, PFGE and antibiotic profiling. The results revealed 41 distinct emm types, the most prevalent of which were emm89 (16 %), emm12 (10 %), emm2 (9 %) and emm1 (8 %). Testing for the presence of superantigens showed that speB (87 %), ssa (36 %) and speG (30 %) were predominant. PFGE detected 39 pulsotypes when a similarity cut-off value of 80 % was implemented. Antibiotic-susceptibility testing against seven different classes of antibiotics showed that 9 % of the isolates were resistant to clindamycin, 23 % were resistant to erythromycin and 4 % showed the macrolide–lincosamide–streptogramin B (MLSB) phenotype. The emergence of tetracycline-resistant strains (37 %) was high when compared with previous reports from Lebanon. This study provided comprehensive evidence of the epidemiology of GAS in Lebanon, highlighting the association between emm types and toxin genes, and providing valuable information about the origin and dissemination of this pathogen.PublishedN/A2016-04-05T09:06:25Z2016-04-05T09:06:25Z20142016-04-05Articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article0022-2615http://hdl.handle.net/10725/3484http://dx.doi.org/10.1099/jmm.0.063412-0Karaky, N. M., Araj, G. F., & Tokajian, S. T. (2014). Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon. Journal of medical microbiology, 63(9), 1197-1204.http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.063412-0enJournal of Medical Microbiologyinfo:eu-repo/semantics/openAccessoai:laur.lau.edu.lb:10725/34842018-05-23T05:56:39Z |
| spellingShingle | Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon Tokajian, Sima T. |
| status_str | publishedVersion |
| title | Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon |
| title_full | Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon |
| title_fullStr | Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon |
| title_full_unstemmed | Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon |
| title_short | Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon |
| title_sort | Molecular characterization of Streptococcus pyogenes group A isolates from a tertiary hospital in Lebanon |
| url | http://hdl.handle.net/10725/3484 http://dx.doi.org/10.1099/jmm.0.063412-0 http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.063412-0 |