Lack of Middle East Respiratory Syndrome Coronavirus Transmission in Rabbits

<p dir="ltr">Middle East respiratory syndrome coronavirus (MERS-CoV) transmission from dromedaries to humans has resulted in major outbreaks in the Middle East. Although some other livestock animal species have been shown to be susceptible to MERS-CoV, it is not fully understood why...

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Main Author: W. Widagdo (18068932) (author)
Other Authors: Nisreen M. A. Okba (7849805) (author), Mathilde Richard (168469) (author), Dennis de Meulder (14774383) (author), Theo M. Bestebroer (7538081) (author), Pascal Lexmond (74807) (author), Elmoubasher A. B. A. Farag (18068935) (author), Mohammed Al-Hajri (18068938) (author), Koert J. Stittelaar (9287795) (author), Leon de Waal (144348) (author), Geert van Amerongen (81939) (author), Judith M. A. van den Brand (7538069) (author), Bart L. Haagmans (7849811) (author), Sander Herfst (327462) (author)
Published: 2019
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author W. Widagdo (18068932)
author2 Nisreen M. A. Okba (7849805)
Mathilde Richard (168469)
Dennis de Meulder (14774383)
Theo M. Bestebroer (7538081)
Pascal Lexmond (74807)
Elmoubasher A. B. A. Farag (18068935)
Mohammed Al-Hajri (18068938)
Koert J. Stittelaar (9287795)
Leon de Waal (144348)
Geert van Amerongen (81939)
Judith M. A. van den Brand (7538069)
Bart L. Haagmans (7849811)
Sander Herfst (327462)
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author_facet W. Widagdo (18068932)
Nisreen M. A. Okba (7849805)
Mathilde Richard (168469)
Dennis de Meulder (14774383)
Theo M. Bestebroer (7538081)
Pascal Lexmond (74807)
Elmoubasher A. B. A. Farag (18068935)
Mohammed Al-Hajri (18068938)
Koert J. Stittelaar (9287795)
Leon de Waal (144348)
Geert van Amerongen (81939)
Judith M. A. van den Brand (7538069)
Bart L. Haagmans (7849811)
Sander Herfst (327462)
author_role author
dc.creator.none.fl_str_mv W. Widagdo (18068932)
Nisreen M. A. Okba (7849805)
Mathilde Richard (168469)
Dennis de Meulder (14774383)
Theo M. Bestebroer (7538081)
Pascal Lexmond (74807)
Elmoubasher A. B. A. Farag (18068935)
Mohammed Al-Hajri (18068938)
Koert J. Stittelaar (9287795)
Leon de Waal (144348)
Geert van Amerongen (81939)
Judith M. A. van den Brand (7538069)
Bart L. Haagmans (7849811)
Sander Herfst (327462)
dc.date.none.fl_str_mv 2019-04-24T03:00:00Z
dc.identifier.none.fl_str_mv 10.3390/v11040381
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Lack_of_Middle_East_Respiratory_Syndrome_Coronavirus_Transmission_in_Rabbits/25303786
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biological sciences
Microbiology
Biomedical and clinical sciences
Cardiovascular medicine and haematology
Medical microbiology
Health sciences
Epidemiology
MERS-coronavirus
transmission
rabbits
dc.title.none.fl_str_mv Lack of Middle East Respiratory Syndrome Coronavirus Transmission in Rabbits
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Middle East respiratory syndrome coronavirus (MERS-CoV) transmission from dromedaries to humans has resulted in major outbreaks in the Middle East. Although some other livestock animal species have been shown to be susceptible to MERS-CoV, it is not fully understood why the spread of the virus in these animal species has not been observed in the field. In this study, we used rabbits to further characterize the transmission potential of MERS-CoV. In line with the presence of MERS-CoV receptor in the rabbit nasal epithelium, high levels of viral RNA were shed from the nose following virus inoculation. However, unlike MERS-CoV-infected dromedaries, these rabbits did not develop clinical manifestations including nasal discharge and did shed only limited amounts of infectious virus from the nose. Consistently, no transmission by contact or airborne routes was observed in rabbits. Our data indicate that despite relatively high viral RNA levels produced, low levels of infectious virus are excreted in the upper respiratory tract of rabbits as compared to dromedary camels, thus resulting in a lack of viral transmission.</p><h2>Other Information</h2><p dir="ltr">Published in: Viruses<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/v11040381" target="_blank">https://dx.doi.org/10.3390/v11040381</a></p>
eu_rights_str_mv openAccess
id Manara2_03f320261f114db80394f5fc69829a35
identifier_str_mv 10.3390/v11040381
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/25303786
publishDate 2019
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rights_invalid_str_mv CC BY 4.0
spelling Lack of Middle East Respiratory Syndrome Coronavirus Transmission in RabbitsW. Widagdo (18068932)Nisreen M. A. Okba (7849805)Mathilde Richard (168469)Dennis de Meulder (14774383)Theo M. Bestebroer (7538081)Pascal Lexmond (74807)Elmoubasher A. B. A. Farag (18068935)Mohammed Al-Hajri (18068938)Koert J. Stittelaar (9287795)Leon de Waal (144348)Geert van Amerongen (81939)Judith M. A. van den Brand (7538069)Bart L. Haagmans (7849811)Sander Herfst (327462)Biological sciencesMicrobiologyBiomedical and clinical sciencesCardiovascular medicine and haematologyMedical microbiologyHealth sciencesEpidemiologyMERS-coronavirustransmissionrabbits<p dir="ltr">Middle East respiratory syndrome coronavirus (MERS-CoV) transmission from dromedaries to humans has resulted in major outbreaks in the Middle East. Although some other livestock animal species have been shown to be susceptible to MERS-CoV, it is not fully understood why the spread of the virus in these animal species has not been observed in the field. In this study, we used rabbits to further characterize the transmission potential of MERS-CoV. In line with the presence of MERS-CoV receptor in the rabbit nasal epithelium, high levels of viral RNA were shed from the nose following virus inoculation. However, unlike MERS-CoV-infected dromedaries, these rabbits did not develop clinical manifestations including nasal discharge and did shed only limited amounts of infectious virus from the nose. Consistently, no transmission by contact or airborne routes was observed in rabbits. Our data indicate that despite relatively high viral RNA levels produced, low levels of infectious virus are excreted in the upper respiratory tract of rabbits as compared to dromedary camels, thus resulting in a lack of viral transmission.</p><h2>Other Information</h2><p dir="ltr">Published in: Viruses<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/v11040381" target="_blank">https://dx.doi.org/10.3390/v11040381</a></p>2019-04-24T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/v11040381https://figshare.com/articles/journal_contribution/Lack_of_Middle_East_Respiratory_Syndrome_Coronavirus_Transmission_in_Rabbits/25303786CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/253037862019-04-24T03:00:00Z
spellingShingle Lack of Middle East Respiratory Syndrome Coronavirus Transmission in Rabbits
W. Widagdo (18068932)
Biological sciences
Microbiology
Biomedical and clinical sciences
Cardiovascular medicine and haematology
Medical microbiology
Health sciences
Epidemiology
MERS-coronavirus
transmission
rabbits
status_str publishedVersion
title Lack of Middle East Respiratory Syndrome Coronavirus Transmission in Rabbits
title_full Lack of Middle East Respiratory Syndrome Coronavirus Transmission in Rabbits
title_fullStr Lack of Middle East Respiratory Syndrome Coronavirus Transmission in Rabbits
title_full_unstemmed Lack of Middle East Respiratory Syndrome Coronavirus Transmission in Rabbits
title_short Lack of Middle East Respiratory Syndrome Coronavirus Transmission in Rabbits
title_sort Lack of Middle East Respiratory Syndrome Coronavirus Transmission in Rabbits
topic Biological sciences
Microbiology
Biomedical and clinical sciences
Cardiovascular medicine and haematology
Medical microbiology
Health sciences
Epidemiology
MERS-coronavirus
transmission
rabbits