Molecular identification and characterization of waterborne protozoa among stranded Dugong dugon

<p dir="ltr">The Persian Gulf hosts the second-largest population of Dugongs. The International Union for the Conservation of Nature (IUCN) lists them as Vulnerable to Extinction species. Incidental fishing, vessel strikes, environmental pollution, habitat degradation, and infectious...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Sonia Boughattas (508422) (author)
مؤلفون آخرون: Layla Ben Ayed (20484314) (author), Hamed Mirjalali (8754816) (author), Marianna Marangi (331934) (author), Dana Albatesh (11721380) (author), Abdelrahman ElGamal (20484317) (author), Ismail Al-Shaikh (13974423) (author), Nayla Al-Neama (20484320) (author), Asmaa A. Althani (4675855) (author), Panagiotis Karanis (5044460) (author), Fatiha M. Benslimane (6695087) (author)
منشور في: 2024
الموضوعات:
الوسوم: إضافة وسم
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author Sonia Boughattas (508422)
author2 Layla Ben Ayed (20484314)
Hamed Mirjalali (8754816)
Marianna Marangi (331934)
Dana Albatesh (11721380)
Abdelrahman ElGamal (20484317)
Ismail Al-Shaikh (13974423)
Nayla Al-Neama (20484320)
Asmaa A. Althani (4675855)
Panagiotis Karanis (5044460)
Fatiha M. Benslimane (6695087)
author2_role author
author
author
author
author
author
author
author
author
author
author_facet Sonia Boughattas (508422)
Layla Ben Ayed (20484314)
Hamed Mirjalali (8754816)
Marianna Marangi (331934)
Dana Albatesh (11721380)
Abdelrahman ElGamal (20484317)
Ismail Al-Shaikh (13974423)
Nayla Al-Neama (20484320)
Asmaa A. Althani (4675855)
Panagiotis Karanis (5044460)
Fatiha M. Benslimane (6695087)
author_role author
dc.creator.none.fl_str_mv Sonia Boughattas (508422)
Layla Ben Ayed (20484314)
Hamed Mirjalali (8754816)
Marianna Marangi (331934)
Dana Albatesh (11721380)
Abdelrahman ElGamal (20484317)
Ismail Al-Shaikh (13974423)
Nayla Al-Neama (20484320)
Asmaa A. Althani (4675855)
Panagiotis Karanis (5044460)
Fatiha M. Benslimane (6695087)
dc.date.none.fl_str_mv 2024-12-16T21:00:00Z
dc.identifier.none.fl_str_mv 10.1016/j.marpolbul.2024.117454
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Molecular_identification_and_characterization_of_waterborne_protozoa_among_stranded_Dugong_dugon/28107254
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Agricultural, veterinary and food sciences
Veterinary sciences
Biological sciences
Ecology
Qatar
Environmental parasites
Vulnerable wild animal
qPCR
Sequencing
dc.title.none.fl_str_mv Molecular identification and characterization of waterborne protozoa among stranded Dugong dugon
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">The Persian Gulf hosts the second-largest population of Dugongs. The International Union for the Conservation of Nature (IUCN) lists them as Vulnerable to Extinction species. Incidental fishing, vessel strikes, environmental pollution, habitat degradation, and infectious diseases currently threaten these sirenians. While research focused on the abovementioned threats, little is known about pathogen infections, particularly waterborne parasites. The current study aimed to investigate the prevalence of the waterborne parasites, namely <i>Blastocystis, Cryptosporidium, Dientamoeba fragilis, Giardia duodenalis, </i>and <i>Toxoplasma gondii </i>in the Qatar Dugongs population. Hence, twenty-three tissues from stranded Dugongs' carcasses were collected postmortem and subjected to DNA extraction and parasite qPCR screening. Only <i>Blastocystis </i>and <i>Cryptosporidium</i> species were detected in different tissues across the tested animals harboring genotypes ST1 and <i>Cryptosporidium hominis</i> IbA9G3, respectively, with the first-time report of <i>Blastocystis</i> sp. within sirenian hosts. Continuous monitoring should be provided to shed lights about the contamination sources of these endangered species and clarify the zoonotic potential transmission routes between wildlife and humans.</p><h2>Other Information</h2><p dir="ltr">Published in: Marine Pollution Bulletin<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.marpolbul.2024.117454" target="_blank">https://dx.doi.org/10.1016/j.marpolbul.2024.117454</a></p>
eu_rights_str_mv openAccess
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identifier_str_mv 10.1016/j.marpolbul.2024.117454
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/28107254
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spelling Molecular identification and characterization of waterborne protozoa among stranded Dugong dugonSonia Boughattas (508422)Layla Ben Ayed (20484314)Hamed Mirjalali (8754816)Marianna Marangi (331934)Dana Albatesh (11721380)Abdelrahman ElGamal (20484317)Ismail Al-Shaikh (13974423)Nayla Al-Neama (20484320)Asmaa A. Althani (4675855)Panagiotis Karanis (5044460)Fatiha M. Benslimane (6695087)Agricultural, veterinary and food sciencesVeterinary sciencesBiological sciencesEcologyQatarEnvironmental parasitesVulnerable wild animalqPCRSequencing<p dir="ltr">The Persian Gulf hosts the second-largest population of Dugongs. The International Union for the Conservation of Nature (IUCN) lists them as Vulnerable to Extinction species. Incidental fishing, vessel strikes, environmental pollution, habitat degradation, and infectious diseases currently threaten these sirenians. While research focused on the abovementioned threats, little is known about pathogen infections, particularly waterborne parasites. The current study aimed to investigate the prevalence of the waterborne parasites, namely <i>Blastocystis, Cryptosporidium, Dientamoeba fragilis, Giardia duodenalis, </i>and <i>Toxoplasma gondii </i>in the Qatar Dugongs population. Hence, twenty-three tissues from stranded Dugongs' carcasses were collected postmortem and subjected to DNA extraction and parasite qPCR screening. Only <i>Blastocystis </i>and <i>Cryptosporidium</i> species were detected in different tissues across the tested animals harboring genotypes ST1 and <i>Cryptosporidium hominis</i> IbA9G3, respectively, with the first-time report of <i>Blastocystis</i> sp. within sirenian hosts. Continuous monitoring should be provided to shed lights about the contamination sources of these endangered species and clarify the zoonotic potential transmission routes between wildlife and humans.</p><h2>Other Information</h2><p dir="ltr">Published in: Marine Pollution Bulletin<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.marpolbul.2024.117454" target="_blank">https://dx.doi.org/10.1016/j.marpolbul.2024.117454</a></p>2024-12-16T21:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.marpolbul.2024.117454https://figshare.com/articles/journal_contribution/Molecular_identification_and_characterization_of_waterborne_protozoa_among_stranded_Dugong_dugon/28107254CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/281072542024-12-16T21:00:00Z
spellingShingle Molecular identification and characterization of waterborne protozoa among stranded Dugong dugon
Sonia Boughattas (508422)
Agricultural, veterinary and food sciences
Veterinary sciences
Biological sciences
Ecology
Qatar
Environmental parasites
Vulnerable wild animal
qPCR
Sequencing
status_str publishedVersion
title Molecular identification and characterization of waterborne protozoa among stranded Dugong dugon
title_full Molecular identification and characterization of waterborne protozoa among stranded Dugong dugon
title_fullStr Molecular identification and characterization of waterborne protozoa among stranded Dugong dugon
title_full_unstemmed Molecular identification and characterization of waterborne protozoa among stranded Dugong dugon
title_short Molecular identification and characterization of waterborne protozoa among stranded Dugong dugon
title_sort Molecular identification and characterization of waterborne protozoa among stranded Dugong dugon
topic Agricultural, veterinary and food sciences
Veterinary sciences
Biological sciences
Ecology
Qatar
Environmental parasites
Vulnerable wild animal
qPCR
Sequencing