Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers
<p>Extracellular vesicles (EVs) are membrane vesicles released from cells to the extracellular space, involved in cell-to-cell communication by the horizontal transfer of biomolecules such as proteins and RNA. Because EVs can cross the blood-brain barrier (BBB), circulating through the bloodst...
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2022
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| _version_ | 1864513564291629056 |
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| author | Houda Yasmine Ali Moussa (16078837) |
| author2 | Nimshitha Manaph (15431074) Gowher Al (16078839) Selma Maacha (501715) Kyung Chul Shin (16078853) Samia M. Ltaief (16078859) Vijay Gupta (209146) Yongfeng Tong (3121338) Janarthanan Ponraj (8535585) Salam Salloum-Asfa (16078909) Said Mansour (8697699) Fouad A. Al-Shaban (16078916) Hyung-Goo Kim (728597) Lawrence W. Stanton (173039) Jean-Charles Grivel (284645) Sara A. Abdulla (16078944) Abeer R. Al-Shammari (16078952) Yongsoo Park (761850) |
| author2_role | author author author author author author author author author author author author author author author author author |
| author_facet | Houda Yasmine Ali Moussa (16078837) Nimshitha Manaph (15431074) Gowher Al (16078839) Selma Maacha (501715) Kyung Chul Shin (16078853) Samia M. Ltaief (16078859) Vijay Gupta (209146) Yongfeng Tong (3121338) Janarthanan Ponraj (8535585) Salam Salloum-Asfa (16078909) Said Mansour (8697699) Fouad A. Al-Shaban (16078916) Hyung-Goo Kim (728597) Lawrence W. Stanton (173039) Jean-Charles Grivel (284645) Sara A. Abdulla (16078944) Abeer R. Al-Shammari (16078952) Yongsoo Park (761850) |
| author_role | author |
| dc.creator.none.fl_str_mv | Houda Yasmine Ali Moussa (16078837) Nimshitha Manaph (15431074) Gowher Al (16078839) Selma Maacha (501715) Kyung Chul Shin (16078853) Samia M. Ltaief (16078859) Vijay Gupta (209146) Yongfeng Tong (3121338) Janarthanan Ponraj (8535585) Salam Salloum-Asfa (16078909) Said Mansour (8697699) Fouad A. Al-Shaban (16078916) Hyung-Goo Kim (728597) Lawrence W. Stanton (173039) Jean-Charles Grivel (284645) Sara A. Abdulla (16078944) Abeer R. Al-Shammari (16078952) Yongsoo Park (761850) |
| dc.date.none.fl_str_mv | 2022-05-17T06:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fnint.2022.879832 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Single_Extracellular_Vesicle_Analysis_Using_Flow_Cytometry_for_Neurological_Disorder_Biomarkers/23358137 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Clinical sciences Neurosciences exosome extracellular vesicle biomarker NCAM neurological disorder |
| dc.title.none.fl_str_mv | Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Extracellular vesicles (EVs) are membrane vesicles released from cells to the extracellular space, involved in cell-to-cell communication by the horizontal transfer of biomolecules such as proteins and RNA. Because EVs can cross the blood-brain barrier (BBB), circulating through the bloodstream and reflecting the cell of origin in terms of disease prognosis and severity, the contents of plasma EVs provide non-invasive biomarkers for neurological disorders. However, neuronal EV markers in blood plasma remain unclear. EVs are very heterogeneous in size and contents, thus bulk analyses of heterogeneous plasma EVs using Western blot and ELISA have limited utility. In this study, using flow cytometry to analyze individual neuronal EVs, we show that our plasma EVs isolated by size exclusion chromatography are mainly CD63-positive exosomes of endosomal origin. As a neuronal EV marker, neural cell adhesion molecule (NCAM) is highly enriched in EVs released from induced pluripotent stem cells (iPSCs)-derived cortical neurons and brain organoids. We identified the subpopulations of plasma EVs that contain NCAM using flow cytometry-based individual EV analysis. Our results suggest that plasma NCAM-positive neuronal EVs can be used to discover biomarkers for neurological disorders. </p> <h2>Other Information</h2> <p>Published in: Frontiers in Integrative Neuroscience<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.3389/fnint.2022.879832" target="_blank">http://dx.doi.org/10.3389/fnint.2022.879832 </a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_064cc49615a1ff18e0997f45ae2579f6 |
| identifier_str_mv | 10.3389/fnint.2022.879832 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/23358137 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder BiomarkersHouda Yasmine Ali Moussa (16078837)Nimshitha Manaph (15431074)Gowher Al (16078839)Selma Maacha (501715)Kyung Chul Shin (16078853)Samia M. Ltaief (16078859)Vijay Gupta (209146)Yongfeng Tong (3121338)Janarthanan Ponraj (8535585)Salam Salloum-Asfa (16078909)Said Mansour (8697699)Fouad A. Al-Shaban (16078916)Hyung-Goo Kim (728597)Lawrence W. Stanton (173039)Jean-Charles Grivel (284645)Sara A. Abdulla (16078944)Abeer R. Al-Shammari (16078952)Yongsoo Park (761850)Biological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesClinical sciencesNeurosciencesexosomeextracellular vesiclebiomarkerNCAMneurological disorder<p>Extracellular vesicles (EVs) are membrane vesicles released from cells to the extracellular space, involved in cell-to-cell communication by the horizontal transfer of biomolecules such as proteins and RNA. Because EVs can cross the blood-brain barrier (BBB), circulating through the bloodstream and reflecting the cell of origin in terms of disease prognosis and severity, the contents of plasma EVs provide non-invasive biomarkers for neurological disorders. However, neuronal EV markers in blood plasma remain unclear. EVs are very heterogeneous in size and contents, thus bulk analyses of heterogeneous plasma EVs using Western blot and ELISA have limited utility. In this study, using flow cytometry to analyze individual neuronal EVs, we show that our plasma EVs isolated by size exclusion chromatography are mainly CD63-positive exosomes of endosomal origin. As a neuronal EV marker, neural cell adhesion molecule (NCAM) is highly enriched in EVs released from induced pluripotent stem cells (iPSCs)-derived cortical neurons and brain organoids. We identified the subpopulations of plasma EVs that contain NCAM using flow cytometry-based individual EV analysis. Our results suggest that plasma NCAM-positive neuronal EVs can be used to discover biomarkers for neurological disorders. </p> <h2>Other Information</h2> <p>Published in: Frontiers in Integrative Neuroscience<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.3389/fnint.2022.879832" target="_blank">http://dx.doi.org/10.3389/fnint.2022.879832 </a></p>2022-05-17T06:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fnint.2022.879832https://figshare.com/articles/journal_contribution/Single_Extracellular_Vesicle_Analysis_Using_Flow_Cytometry_for_Neurological_Disorder_Biomarkers/23358137CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/233581372022-05-17T06:00:00Z |
| spellingShingle | Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers Houda Yasmine Ali Moussa (16078837) Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Clinical sciences Neurosciences exosome extracellular vesicle biomarker NCAM neurological disorder |
| status_str | publishedVersion |
| title | Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers |
| title_full | Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers |
| title_fullStr | Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers |
| title_full_unstemmed | Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers |
| title_short | Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers |
| title_sort | Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers |
| topic | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Clinical sciences Neurosciences exosome extracellular vesicle biomarker NCAM neurological disorder |