An optimized protocol for the preparation of blood immune cells for transmission electron microscopy
<p>Transmission electron microscopy (TEM) is a powerful technique that enables visualization of structural details inside cells. Prior to TEM imaging, biological samples must undergo several preparation steps that are optimized according to the sample type. Currently, there are limited protoco...
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2023
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| _version_ | 1864513528799428608 |
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| author | Nimshitha Pavathuparambil Abdul Manaph (17870420) |
| author2 | Samia M. Ltaief (11405336) Wared Nour-Eldine (13904298) Janarthanan Ponraj (8535585) John Agcaoili (17870423) Said Mansour (8697699) Abeer R. Al-Shammari (11405339) |
| author2_role | author author author author author author |
| author_facet | Nimshitha Pavathuparambil Abdul Manaph (17870420) Samia M. Ltaief (11405336) Wared Nour-Eldine (13904298) Janarthanan Ponraj (8535585) John Agcaoili (17870423) Said Mansour (8697699) Abeer R. Al-Shammari (11405339) |
| author_role | author |
| dc.creator.none.fl_str_mv | Nimshitha Pavathuparambil Abdul Manaph (17870420) Samia M. Ltaief (11405336) Wared Nour-Eldine (13904298) Janarthanan Ponraj (8535585) John Agcaoili (17870423) Said Mansour (8697699) Abeer R. Al-Shammari (11405339) |
| dc.date.none.fl_str_mv | 2023-10-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.micron.2023.103517 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/An_optimized_protocol_for_the_preparation_of_blood_immune_cells_for_transmission_electron_microscopy/25108010 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Physical sciences Astronomical sciences Blood Immune cells Sample preparation Transmission electron microscopy TEM |
| dc.title.none.fl_str_mv | An optimized protocol for the preparation of blood immune cells for transmission electron microscopy |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Transmission electron microscopy (TEM) is a powerful technique that enables visualization of structural details inside cells. Prior to TEM imaging, biological samples must undergo several preparation steps that are optimized according to the sample type. Currently, there are limited protocols for the preparation of blood samples for TEM imaging. Here, we provide a detailed step-by-step method for preparing blood samples for TEM imaging. This protocol enables robust visualization of the ultrastructures of blood immune cells. In addition, we describe the typical cellular features that can be used to distinguish between different immune cells in the blood, such as neutrophils, eosinophils, monocytes, and lymphocytes. This protocol is useful for studying ultrastructural changes in blood immune cells under various physiological and disease conditions.</p><h2>Other Information</h2> <p> Published in: Micron<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.micron.2023.103517" target="_blank">https://dx.doi.org/10.1016/j.micron.2023.103517</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_07eacdb601708ed47b2df78b17b04768 |
| identifier_str_mv | 10.1016/j.micron.2023.103517 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25108010 |
| publishDate | 2023 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | An optimized protocol for the preparation of blood immune cells for transmission electron microscopyNimshitha Pavathuparambil Abdul Manaph (17870420)Samia M. Ltaief (11405336)Wared Nour-Eldine (13904298)Janarthanan Ponraj (8535585)John Agcaoili (17870423)Said Mansour (8697699)Abeer R. Al-Shammari (11405339)Biological sciencesBiochemistry and cell biologyPhysical sciencesAstronomical sciencesBloodImmune cellsSample preparationTransmission electron microscopyTEM<p>Transmission electron microscopy (TEM) is a powerful technique that enables visualization of structural details inside cells. Prior to TEM imaging, biological samples must undergo several preparation steps that are optimized according to the sample type. Currently, there are limited protocols for the preparation of blood samples for TEM imaging. Here, we provide a detailed step-by-step method for preparing blood samples for TEM imaging. This protocol enables robust visualization of the ultrastructures of blood immune cells. In addition, we describe the typical cellular features that can be used to distinguish between different immune cells in the blood, such as neutrophils, eosinophils, monocytes, and lymphocytes. This protocol is useful for studying ultrastructural changes in blood immune cells under various physiological and disease conditions.</p><h2>Other Information</h2> <p> Published in: Micron<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.micron.2023.103517" target="_blank">https://dx.doi.org/10.1016/j.micron.2023.103517</a></p>2023-10-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.micron.2023.103517https://figshare.com/articles/journal_contribution/An_optimized_protocol_for_the_preparation_of_blood_immune_cells_for_transmission_electron_microscopy/25108010CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/251080102023-10-01T00:00:00Z |
| spellingShingle | An optimized protocol for the preparation of blood immune cells for transmission electron microscopy Nimshitha Pavathuparambil Abdul Manaph (17870420) Biological sciences Biochemistry and cell biology Physical sciences Astronomical sciences Blood Immune cells Sample preparation Transmission electron microscopy TEM |
| status_str | publishedVersion |
| title | An optimized protocol for the preparation of blood immune cells for transmission electron microscopy |
| title_full | An optimized protocol for the preparation of blood immune cells for transmission electron microscopy |
| title_fullStr | An optimized protocol for the preparation of blood immune cells for transmission electron microscopy |
| title_full_unstemmed | An optimized protocol for the preparation of blood immune cells for transmission electron microscopy |
| title_short | An optimized protocol for the preparation of blood immune cells for transmission electron microscopy |
| title_sort | An optimized protocol for the preparation of blood immune cells for transmission electron microscopy |
| topic | Biological sciences Biochemistry and cell biology Physical sciences Astronomical sciences Blood Immune cells Sample preparation Transmission electron microscopy TEM |