Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics
<p dir="ltr">Kit-based assays, such as AbsoluteIDQTM p150, are widely used in large cohort studies and provide a standardized method to quantify blood concentrations of phosphatidylcholines (PCs). Many disease-relevant associations of PCs were reported using this method. However, the...
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| مؤلفون آخرون: | , , , , , , , , , , , , |
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2019
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| _version_ | 1864513511422427136 |
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| author | Jan D. Quell (18808240) |
| author2 | Werner Römisch-Margl (127480) Mark Haid (4623739) Jan Krumsiek (127460) Thomas Skurk (1985206) Anna Halama (545988) Nisha Stephan (17445912) Jerzy Adamski (127482) Hans Hauner (476151) Dennis Mook-Kanamori (5520143) Robert P. Mohney (127471) Hannelore Daniel (198670) Karsten Suhre (67967) Gabi Kastenmüller (127487) |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Jan D. Quell (18808240) Werner Römisch-Margl (127480) Mark Haid (4623739) Jan Krumsiek (127460) Thomas Skurk (1985206) Anna Halama (545988) Nisha Stephan (17445912) Jerzy Adamski (127482) Hans Hauner (476151) Dennis Mook-Kanamori (5520143) Robert P. Mohney (127471) Hannelore Daniel (198670) Karsten Suhre (67967) Gabi Kastenmüller (127487) |
| author_role | author |
| dc.creator.none.fl_str_mv | Jan D. Quell (18808240) Werner Römisch-Margl (127480) Mark Haid (4623739) Jan Krumsiek (127460) Thomas Skurk (1985206) Anna Halama (545988) Nisha Stephan (17445912) Jerzy Adamski (127482) Hans Hauner (476151) Dennis Mook-Kanamori (5520143) Robert P. Mohney (127471) Hannelore Daniel (198670) Karsten Suhre (67967) Gabi Kastenmüller (127487) |
| dc.date.none.fl_str_mv | 2019-06-08T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/metabo9060109 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Characterization_of_Bulk_Phosphatidylcholine_Compositions_in_Human_Plasma_Using_Side-Chain_Resolving_Lipidomics/26019430 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Medical biochemistry and metabolomics metabolomics lipidomics phospholipids isobaric phosphatidylcholines lipid species fatty acid composition platform comparison harmonization imputation |
| dc.title.none.fl_str_mv | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Kit-based assays, such as AbsoluteIDQTM p150, are widely used in large cohort studies and provide a standardized method to quantify blood concentrations of phosphatidylcholines (PCs). Many disease-relevant associations of PCs were reported using this method. However, their interpretation is hampered by lack of functionally-relevant information on the detailed fatty acid side-chain compositions as only the total number of carbon atoms and double bonds is identified by the kit. To enable more substantiated interpretations, we characterized these PC sums using the side-chain resolving LipidyzerTM platform, analyzing 223 samples in parallel to the AbsoluteIDQTM. Combining these datasets, we estimated the quantitative composition of PC sums and subsequently tested their replication in an independent cohort. We identified major constituents of 28 PC sums, revealing also various unexpected compositions. As an example, PC 16:0_22:5 accounted for more than 50% of the PC sum with in total 38 carbon atoms and 5 double bonds (PC aa 38:5). For 13 PC sums, we found relatively high abundances of odd-chain fatty acids. In conclusion, our study provides insights in PC compositions in human plasma, facilitating interpretation of existing epidemiological data sets and potentially enabling imputation of PC compositions for future meta-analyses of lipidomics data.</p><h2>Other Information</h2><p dir="ltr">Published in: Metabolites<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/metabo9060109" target="_blank">https://dx.doi.org/10.3390/metabo9060109</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_08b2ce7b92b8b9be3b2dc7cdde83737c |
| identifier_str_mv | 10.3390/metabo9060109 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26019430 |
| publishDate | 2019 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving LipidomicsJan D. Quell (18808240)Werner Römisch-Margl (127480)Mark Haid (4623739)Jan Krumsiek (127460)Thomas Skurk (1985206)Anna Halama (545988)Nisha Stephan (17445912)Jerzy Adamski (127482)Hans Hauner (476151)Dennis Mook-Kanamori (5520143)Robert P. Mohney (127471)Hannelore Daniel (198670)Karsten Suhre (67967)Gabi Kastenmüller (127487)Biomedical and clinical sciencesClinical sciencesMedical biochemistry and metabolomicsmetabolomicslipidomicsphospholipidsisobaric phosphatidylcholineslipid speciesfatty acid compositionplatform comparisonharmonizationimputation<p dir="ltr">Kit-based assays, such as AbsoluteIDQTM p150, are widely used in large cohort studies and provide a standardized method to quantify blood concentrations of phosphatidylcholines (PCs). Many disease-relevant associations of PCs were reported using this method. However, their interpretation is hampered by lack of functionally-relevant information on the detailed fatty acid side-chain compositions as only the total number of carbon atoms and double bonds is identified by the kit. To enable more substantiated interpretations, we characterized these PC sums using the side-chain resolving LipidyzerTM platform, analyzing 223 samples in parallel to the AbsoluteIDQTM. Combining these datasets, we estimated the quantitative composition of PC sums and subsequently tested their replication in an independent cohort. We identified major constituents of 28 PC sums, revealing also various unexpected compositions. As an example, PC 16:0_22:5 accounted for more than 50% of the PC sum with in total 38 carbon atoms and 5 double bonds (PC aa 38:5). For 13 PC sums, we found relatively high abundances of odd-chain fatty acids. In conclusion, our study provides insights in PC compositions in human plasma, facilitating interpretation of existing epidemiological data sets and potentially enabling imputation of PC compositions for future meta-analyses of lipidomics data.</p><h2>Other Information</h2><p dir="ltr">Published in: Metabolites<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/metabo9060109" target="_blank">https://dx.doi.org/10.3390/metabo9060109</a></p>2019-06-08T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/metabo9060109https://figshare.com/articles/journal_contribution/Characterization_of_Bulk_Phosphatidylcholine_Compositions_in_Human_Plasma_Using_Side-Chain_Resolving_Lipidomics/26019430CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/260194302019-06-08T03:00:00Z |
| spellingShingle | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics Jan D. Quell (18808240) Biomedical and clinical sciences Clinical sciences Medical biochemistry and metabolomics metabolomics lipidomics phospholipids isobaric phosphatidylcholines lipid species fatty acid composition platform comparison harmonization imputation |
| status_str | publishedVersion |
| title | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
| title_full | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
| title_fullStr | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
| title_full_unstemmed | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
| title_short | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
| title_sort | Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics |
| topic | Biomedical and clinical sciences Clinical sciences Medical biochemistry and metabolomics metabolomics lipidomics phospholipids isobaric phosphatidylcholines lipid species fatty acid composition platform comparison harmonization imputation |