Vitamin D<sub>3</sub> metabolite ratio as an indicator of vitamin D status and its association with diabetes complications

<h3>Background</h3><p dir="ltr">Vitamin D deficiency is diagnosed by total serum 25-hydroxyvitamin D (25(OH)D) concentration and is associated with poor health and increased mortality; however, some populations have low 25(OH) D concentrations without manifestations of vi...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Lina H. M. Ahmed (9571561) (author)
مؤلفون آخرون: Alexandra E. Butler (6189536) (author), Soha R. Dargham (3613250) (author), Aishah Latif (9424672) (author), Omar M. Chidiac (9424678) (author), Stephen L. Atkin (6684368) (author), Charbel Abi Khalil (781797) (author)
منشور في: 2020
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الوصف
الملخص:<h3>Background</h3><p dir="ltr">Vitamin D deficiency is diagnosed by total serum 25-hydroxyvitamin D (25(OH)D) concentration and is associated with poor health and increased mortality; however, some populations have low 25(OH) D concentrations without manifestations of vitamin D deficiency. The Vitamin D Metabolite Ratio (VMR) has been suggested as a superior indicator of vitamin D status. Therefore, VMR was determined in a population with type 2 diabetes at high risk for vitamin D deficiency and correlated with diabetic complications.</p><h3>Research design and methods</h3><p dir="ltr">Four hundred sisty patients with type 2 diabetes (T2D) were recruited, all were vitamin D3 supplement naive. Plasma concentration of 25-hydroxyvitamin D<sub>3</sub> (25(OH)D<sub>3</sub>) and its metabolites 1,25-dihydroxyvitamin D<sub>3</sub> (1,25(OH)<sub>2</sub>D<sub>3</sub>) and 24,25-dihydroxyvitamin D<sub>3</sub> (24,25(OH)<sub>2</sub>D<sub>3</sub>) and its epimer, 3-epi-25-hydroxyvitamin D<sub>3</sub> (3-epi-25(OH)D<sub>3</sub>), were measured by LC-MS/MS analysis. VMR-1 was calculated as a ratio of 24,25(OH)<sub>2</sub>D<sub>3</sub>:25(OH)D<sub>3</sub>; VMR-2 as a ratio of 1,25(OH)<sub>2</sub>D<sub>3</sub>:25(OH)D<sub>3</sub>; VMR-3 was calculated as a ratio of 3-epi-25(OH)D<sub>3</sub>: 25(OH)D<sub>3</sub>.</p><h3>Results</h3><p dir="ltr">An association means that there were significant differences between the ratios found for those with versus those without the various diabetic complications studied. VMR-<sub>1</sub> was associated with diabetic retinopathy (p = 0.001) and peripheral artery disease (p = 0.012); VMR-<sub>2</sub> associated with hypertension (p < 0.001), dyslipidemia (p < 0.001), diabetic retinopathy (p < 0.001), diabetic neuropathy (p < 0.001), coronary artery disease (p = 0.001) and stroke (p < 0.05). VMR-<sub>3</sub> associated with hypertension (p < 0.05), dyslipidemia (p < 0.001) and coronary artery disease (p < 0.05).</p><h3>Conclusions</h3><p dir="ltr">In this cross sectional study, whilst not causal, VMR-<sub>2</sub> was shown to be the superior predictor of diabetic and cardiovascular complications though not demonstrative of causality in this cross-sectional study population over VMR-<sub>1</sub>, VMR-<sub>3</sub> and the individual vitamin D concentration measurements; VMR-<sub>2</sub> associated with both microvascular and cardiovascular indices and therefore may have utility in predicting the development of diabetic complications.</p><h2>Other Information</h2><p dir="ltr">Published in: BMC Endocrine Disorders<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s12902-020-00641-1" target="_blank">https://dx.doi.org/10.1186/s12902-020-00641-1</a></p>