Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates
<p>Malaria is one of the important vector-borne diseases with high fatality rates in tropical countries. The pattern of emergence and spread of novel antigenic variants, leading to escape of vaccine-induced immunity might be factors responsible for severe malaria. A high level of polymorphism...
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| مؤلفون آخرون: | , , , , , , , |
| منشور في: |
2019
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| _version_ | 1864513511799914496 |
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| author | Hargobinder Kaur (5821277) |
| author2 | Rakesh Sehgal (324268) Archit Kumar (5821286) Alka Sehgal (5821295) Praveen K. Bharti (7409732) Devendra Bansal (255958) Pradyumna K. Mohapatra (4336252) Jagadish Mahanta (625026) Ali A. Sultan (7409735) |
| author2_role | author author author author author author author author |
| author_facet | Hargobinder Kaur (5821277) Rakesh Sehgal (324268) Archit Kumar (5821286) Alka Sehgal (5821295) Praveen K. Bharti (7409732) Devendra Bansal (255958) Pradyumna K. Mohapatra (4336252) Jagadish Mahanta (625026) Ali A. Sultan (7409735) |
| author_role | author |
| dc.creator.none.fl_str_mv | Hargobinder Kaur (5821277) Rakesh Sehgal (324268) Archit Kumar (5821286) Alka Sehgal (5821295) Praveen K. Bharti (7409732) Devendra Bansal (255958) Pradyumna K. Mohapatra (4336252) Jagadish Mahanta (625026) Ali A. Sultan (7409735) |
| dc.date.none.fl_str_mv | 2019-09-06T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1186/s12936-019-2939-z |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Exploration_of_genetic_diversity_of_Plasmodium_vivax_circumsporozoite_protein_Pvcsp_and_Plasmodium_vivax_sexual_stage_antigen_Pvs25_among_North_Indian_isolates/26020963 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Genetics Biomedical and clinical sciences Medical microbiology Plasmodium vivax Genetic diversity Pvcsp Pvs25 |
| dc.title.none.fl_str_mv | Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Malaria is one of the important vector-borne diseases with high fatality rates in tropical countries. The pattern of emergence and spread of novel antigenic variants, leading to escape of vaccine-induced immunity might be factors responsible for severe malaria. A high level of polymorphism has been reported among malarial antigens which are under selection pressure imposed by host immunity. There are limited reports available on comparative stage-specific genetic diversity among Plasmodium vivax candidate genes in complicated vivax malaria. The present study was planned to study genetic diversity (Pvcsp and Pvs25) among complicated and uncomplicated P. vivax isolates. Pvcsp and Pvs2-specific PCRs and DNA sequencing were performed on P. vivax PCR positive samples. Genetic diversity was analysed using appropriate software. The present study was carried out on 143 P. vivax clinical isolates, collected from Postgraduate Institute of Medical Education and Research, Chandigarh. Among the classic and variant types of Pvcsp, the VK210 (99%; 115/116) was found to be predominant in both complicated and uncomplicated group isolates. Out of the various peptide repeat motifs (PRMs) observed, GDRADGQPA (PRM1) and GDRAAGQPA (PRM2) was the most widely distributed among the P. vivax isolates. Whereas among the Pvs25 isolates, 100% of double mutants (E97Q/I130T) in both the complicated (45/45) as well as in the uncomplicated (81/81) group was observed. An analysis of genetic variability enables an understanding of the role of genetic variants in severe vivax malaria.</p><h2>Other Information</h2> <p> Published in: Malaria Journal<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s12936-019-2939-z" target="_blank">https://dx.doi.org/10.1186/s12936-019-2939-z</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_0a73ff795e4081e5edc09c6a77e4678e |
| identifier_str_mv | 10.1186/s12936-019-2939-z |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26020963 |
| publishDate | 2019 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolatesHargobinder Kaur (5821277)Rakesh Sehgal (324268)Archit Kumar (5821286)Alka Sehgal (5821295)Praveen K. Bharti (7409732)Devendra Bansal (255958)Pradyumna K. Mohapatra (4336252)Jagadish Mahanta (625026)Ali A. Sultan (7409735)Biological sciencesBiochemistry and cell biologyGeneticsBiomedical and clinical sciencesMedical microbiologyPlasmodium vivaxGenetic diversityPvcspPvs25<p>Malaria is one of the important vector-borne diseases with high fatality rates in tropical countries. The pattern of emergence and spread of novel antigenic variants, leading to escape of vaccine-induced immunity might be factors responsible for severe malaria. A high level of polymorphism has been reported among malarial antigens which are under selection pressure imposed by host immunity. There are limited reports available on comparative stage-specific genetic diversity among Plasmodium vivax candidate genes in complicated vivax malaria. The present study was planned to study genetic diversity (Pvcsp and Pvs25) among complicated and uncomplicated P. vivax isolates. Pvcsp and Pvs2-specific PCRs and DNA sequencing were performed on P. vivax PCR positive samples. Genetic diversity was analysed using appropriate software. The present study was carried out on 143 P. vivax clinical isolates, collected from Postgraduate Institute of Medical Education and Research, Chandigarh. Among the classic and variant types of Pvcsp, the VK210 (99%; 115/116) was found to be predominant in both complicated and uncomplicated group isolates. Out of the various peptide repeat motifs (PRMs) observed, GDRADGQPA (PRM1) and GDRAAGQPA (PRM2) was the most widely distributed among the P. vivax isolates. Whereas among the Pvs25 isolates, 100% of double mutants (E97Q/I130T) in both the complicated (45/45) as well as in the uncomplicated (81/81) group was observed. An analysis of genetic variability enables an understanding of the role of genetic variants in severe vivax malaria.</p><h2>Other Information</h2> <p> Published in: Malaria Journal<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s12936-019-2939-z" target="_blank">https://dx.doi.org/10.1186/s12936-019-2939-z</a></p>2019-09-06T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1186/s12936-019-2939-zhttps://figshare.com/articles/journal_contribution/Exploration_of_genetic_diversity_of_Plasmodium_vivax_circumsporozoite_protein_Pvcsp_and_Plasmodium_vivax_sexual_stage_antigen_Pvs25_among_North_Indian_isolates/26020963CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/260209632019-09-06T03:00:00Z |
| spellingShingle | Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates Hargobinder Kaur (5821277) Biological sciences Biochemistry and cell biology Genetics Biomedical and clinical sciences Medical microbiology Plasmodium vivax Genetic diversity Pvcsp Pvs25 |
| status_str | publishedVersion |
| title | Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates |
| title_full | Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates |
| title_fullStr | Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates |
| title_full_unstemmed | Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates |
| title_short | Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates |
| title_sort | Exploration of genetic diversity of Plasmodium vivax circumsporozoite protein (Pvcsp) and Plasmodium vivax sexual stage antigen (Pvs25) among North Indian isolates |
| topic | Biological sciences Biochemistry and cell biology Genetics Biomedical and clinical sciences Medical microbiology Plasmodium vivax Genetic diversity Pvcsp Pvs25 |