Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA

Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA

<p dir="ltr">To circumvent the limited availability of RNA extraction reagents, we aimed to develop a protocol for direct RT-qPCR to detect SARS-CoV-2 in nasopharyngeal swabs without RNA extraction. Nasopharyngeal specimens positive for SARS-CoV-2 and other coronaviruses collected in...

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Bibliographic Details
Main Author: Mohammad Rubayet Hasan (8602080) (author)
Other Authors: Faheem Mirza (9167127) (author), Hamad Al-Hail (9167130) (author), Sathyavathi Sundararaju (8602086) (author), Thabisile Xaba (9167133) (author), Muhammad Iqbal (114924) (author), Hashim Alhussain (9167136) (author), Hadi Mohamad Yassine (9167139) (author), Andres Perez-Lopez (5750300) (author), Patrick Tang (239534) (author)
Published: 2020
Subjects:
Biological sciences
Microbiology
Zoology
SARS CoV 2
RNA extraction
Virus testing
COVID 19
Polymerase chain reaction
Reverse transcriptase-polymerase chain reaction
Heat treatment
Nucleases
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