Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study
<p dir="ltr">α‐Synuclein is the major component of Lewy bodies and a candidate biomarker for neurodegenerative diseases in which Lewy bodies are common, including Parkinson's disease and dementia with Lewy bodies. A large body of literature suggests that these disorders are char...
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| مؤلفون آخرون: | , , , , , , , , , , , , , , , , , , , , , , |
| منشور في: |
2018
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| _version_ | 1864513512293793792 |
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| author | Brit Mollenhauer (482499) |
| author2 | Frederick DuBois Bowman (18891814) Daniel Drake (8359332) Jimmy Duong (14995710) Kaj Blennow (58615) Omar El‐Agnaf (14778913) Leslie M. Shaw (7164809) Jennifer Masucci (18891817) Peggy Taylor (4377463) Robert M. Umek (18891820) Jill M. Dunty (8626323) Chris L. Smith (15077673) Erik Stoops (3538745) Hugo Vanderstichele (3172662) Adrian W. Schmid (18891823) Marc Moniatte (832849) Jing Zhang (23775) Niels Kruse (659840) Hilal A. Lashuel (261037) Charlotte Teunissen (2166505) Tanja Schubert (18083338) Kuldip D. Dave (10947823) Samantha J. Hutten (11534950) Henrik Zetterberg (144718) |
| author2_role | author author author author author author author author author author author author author author author author author author author author author author author |
| author_facet | Brit Mollenhauer (482499) Frederick DuBois Bowman (18891814) Daniel Drake (8359332) Jimmy Duong (14995710) Kaj Blennow (58615) Omar El‐Agnaf (14778913) Leslie M. Shaw (7164809) Jennifer Masucci (18891817) Peggy Taylor (4377463) Robert M. Umek (18891820) Jill M. Dunty (8626323) Chris L. Smith (15077673) Erik Stoops (3538745) Hugo Vanderstichele (3172662) Adrian W. Schmid (18891823) Marc Moniatte (832849) Jing Zhang (23775) Niels Kruse (659840) Hilal A. Lashuel (261037) Charlotte Teunissen (2166505) Tanja Schubert (18083338) Kuldip D. Dave (10947823) Samantha J. Hutten (11534950) Henrik Zetterberg (144718) |
| author_role | author |
| dc.creator.none.fl_str_mv | Brit Mollenhauer (482499) Frederick DuBois Bowman (18891814) Daniel Drake (8359332) Jimmy Duong (14995710) Kaj Blennow (58615) Omar El‐Agnaf (14778913) Leslie M. Shaw (7164809) Jennifer Masucci (18891817) Peggy Taylor (4377463) Robert M. Umek (18891820) Jill M. Dunty (8626323) Chris L. Smith (15077673) Erik Stoops (3538745) Hugo Vanderstichele (3172662) Adrian W. Schmid (18891823) Marc Moniatte (832849) Jing Zhang (23775) Niels Kruse (659840) Hilal A. Lashuel (261037) Charlotte Teunissen (2166505) Tanja Schubert (18083338) Kuldip D. Dave (10947823) Samantha J. Hutten (11534950) Henrik Zetterberg (144718) |
| dc.date.none.fl_str_mv | 2018-11-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1111/jnc.14569 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Antibody_based_methods_for_the_measurement_of_synuclein_concentration_in_human_cerebrospinal_fluid_method_comparison_and_round_robin_study/26114620 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Neurosciences biomarker cerebrospinal fluid enzyme-linked immunoabsorbent assay mass spectrometry round robin a-synuclein |
| dc.title.none.fl_str_mv | Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">α‐Synuclein is the major component of Lewy bodies and a candidate biomarker for neurodegenerative diseases in which Lewy bodies are common, including Parkinson's disease and dementia with Lewy bodies. A large body of literature suggests that these disorders are characterized by reduced concentrations of α‐synuclein in cerebrospinal fluid (CSF), with overlapping concentrations compared to healthy controls and variability across studies. Several reasons can account for this variability, including technical ones, such as inter‐assay and inter‐laboratory variation (reproducibility). We compared four immunochemical methods for the quantification of α‐synuclein concentration in 50 unique CSF samples. All methods were designed to capture most of the existing α‐synuclein forms in CSF (‘total’ α‐synuclein). Each of the four methods showed high analytical precision, excellent correlation between laboratories (R2 0.83–0.99), and good correlation with each other (R2 0.64–0.93), although the slopes of the regression lines were different between the four immunoassays. The use of common reference CSF samples decreased the differences in α‐synuclein concentration between detection methods and technologies. Pilot data on an immunoprecipitation mass spectrometry (IP‐MS) method is also presented. Our results suggest that the four immunochemical methods and the IP‐MS method measure similar forms of α‐synuclein and that a common reference material would allow harmonization of results between immunoassays.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Neurochemistry<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1111/jnc.14569" target="_blank">https://dx.doi.org/10.1111/jnc.14569</a></p><p dir="ltr"><br></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_143fd9d9a1d36c47d39e5e957c7c0f10 |
| identifier_str_mv | 10.1111/jnc.14569 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26114620 |
| publishDate | 2018 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin studyBrit Mollenhauer (482499)Frederick DuBois Bowman (18891814)Daniel Drake (8359332)Jimmy Duong (14995710)Kaj Blennow (58615)Omar El‐Agnaf (14778913)Leslie M. Shaw (7164809)Jennifer Masucci (18891817)Peggy Taylor (4377463)Robert M. Umek (18891820)Jill M. Dunty (8626323)Chris L. Smith (15077673)Erik Stoops (3538745)Hugo Vanderstichele (3172662)Adrian W. Schmid (18891823)Marc Moniatte (832849)Jing Zhang (23775)Niels Kruse (659840)Hilal A. Lashuel (261037)Charlotte Teunissen (2166505)Tanja Schubert (18083338)Kuldip D. Dave (10947823)Samantha J. Hutten (11534950)Henrik Zetterberg (144718)Biological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesNeurosciencesbiomarkercerebrospinal fluidenzyme-linked immunoabsorbent assaymass spectrometryround robina-synuclein<p dir="ltr">α‐Synuclein is the major component of Lewy bodies and a candidate biomarker for neurodegenerative diseases in which Lewy bodies are common, including Parkinson's disease and dementia with Lewy bodies. A large body of literature suggests that these disorders are characterized by reduced concentrations of α‐synuclein in cerebrospinal fluid (CSF), with overlapping concentrations compared to healthy controls and variability across studies. Several reasons can account for this variability, including technical ones, such as inter‐assay and inter‐laboratory variation (reproducibility). We compared four immunochemical methods for the quantification of α‐synuclein concentration in 50 unique CSF samples. All methods were designed to capture most of the existing α‐synuclein forms in CSF (‘total’ α‐synuclein). Each of the four methods showed high analytical precision, excellent correlation between laboratories (R2 0.83–0.99), and good correlation with each other (R2 0.64–0.93), although the slopes of the regression lines were different between the four immunoassays. The use of common reference CSF samples decreased the differences in α‐synuclein concentration between detection methods and technologies. Pilot data on an immunoprecipitation mass spectrometry (IP‐MS) method is also presented. Our results suggest that the four immunochemical methods and the IP‐MS method measure similar forms of α‐synuclein and that a common reference material would allow harmonization of results between immunoassays.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Neurochemistry<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1111/jnc.14569" target="_blank">https://dx.doi.org/10.1111/jnc.14569</a></p><p dir="ltr"><br></p>2018-11-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1111/jnc.14569https://figshare.com/articles/journal_contribution/Antibody_based_methods_for_the_measurement_of_synuclein_concentration_in_human_cerebrospinal_fluid_method_comparison_and_round_robin_study/26114620CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/261146202018-11-01T00:00:00Z |
| spellingShingle | Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study Brit Mollenhauer (482499) Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Neurosciences biomarker cerebrospinal fluid enzyme-linked immunoabsorbent assay mass spectrometry round robin a-synuclein |
| status_str | publishedVersion |
| title | Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study |
| title_full | Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study |
| title_fullStr | Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study |
| title_full_unstemmed | Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study |
| title_short | Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study |
| title_sort | Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study |
| topic | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Neurosciences biomarker cerebrospinal fluid enzyme-linked immunoabsorbent assay mass spectrometry round robin a-synuclein |