Investigation of the Effect of Exendin-4 on Oleic Acid-Induced Steatosis in HepG2 Cells Using Fourier Transform Infrared Spectroscopy
<div><p>Non-alcoholic fatty liver disease (NAFLD) is a common liver lesion that is untreatable with medications. Glucagon-like peptide-1 receptor (GLP-1R) agonists have recently emerged as a potential NAFLD pharmacotherapy. However, the molecular mechanisms underlying these drugs’ benefi...
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2022
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| _version_ | 1864513519219638272 |
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| author | Olfa Khalifa (10914452) |
| author2 | Kamal H. Mroue (18372924) Raghvendra Mall (581171) Ehsan Ullah (2698921) Nayla S. Al-Akl (18372927) Abdelilah Arredouani (10914455) |
| author2_role | author author author author author |
| author_facet | Olfa Khalifa (10914452) Kamal H. Mroue (18372924) Raghvendra Mall (581171) Ehsan Ullah (2698921) Nayla S. Al-Akl (18372927) Abdelilah Arredouani (10914455) |
| author_role | author |
| dc.creator.none.fl_str_mv | Olfa Khalifa (10914452) Kamal H. Mroue (18372924) Raghvendra Mall (581171) Ehsan Ullah (2698921) Nayla S. Al-Akl (18372927) Abdelilah Arredouani (10914455) |
| dc.date.none.fl_str_mv | 2022-10-20T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/biomedicines10102652 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Investigation_of_the_Effect_of_Exendin-4_on_Oleic_Acid-Induced_Steatosis_in_HepG2_Cells_Using_Fourier_Transform_Infrared_Spectroscopy/25609866 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Genetics non-alcoholic fatty liver disease steatosis Oil Red O staining Fourier transform-infrared spectroscopy principal component analysis |
| dc.title.none.fl_str_mv | Investigation of the Effect of Exendin-4 on Oleic Acid-Induced Steatosis in HepG2 Cells Using Fourier Transform Infrared Spectroscopy |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>Non-alcoholic fatty liver disease (NAFLD) is a common liver lesion that is untreatable with medications. Glucagon-like peptide-1 receptor (GLP-1R) agonists have recently emerged as a potential NAFLD pharmacotherapy. However, the molecular mechanisms underlying these drugs’ beneficial effects are not fully understood. Using Fourier transform infrared (FTIR) spectroscopy, we sought to investigate the biochemical changes in a steatosis cell model treated or not with the GLP-1R agonist Exendin-4 (Ex-4). HepG2 cells were made steatotic with 400 µM of oleic acid and then treated with 200 nM Ex-4 in order to reduce lipid accumulation. We quantified steatosis using the Oil Red O staining method. We investigated the biochemical alterations induced by steatosis and Ex-4 treatment using Fourier transform infrared (FTIR) spectroscopy and chemometric analyses. Analysis of the Oil Red O staining showed that Ex-4 significantly reduces steatosis. This reduction was confirmed by FTIR analysis, as the phospholipid band (C=O) at 1740 cm−1 in Ex-4 treated cells is significantly decreased compared to steatotic cells. The principal component analysis score plots for both the lipid and protein regions showed that the untreated and Ex-4-treated samples, while still separated, are clustered close to each other, far from the steatotic cells. The biochemical and structural changes induced by OA-induced lipotoxicity are at least partially reversed upon Ex-4 treatment. FTIR and chemometric analyses revealed that Ex-4 significantly reduces OA-induced lipid accumulation, and Ex-4 also restored the lipid and protein biochemical alterations caused by lipotoxicity-induced oxidative stress. In combination with chemometric analyses, FTIR spectroscopy may offer new approaches for investigating the mechanisms underpinning NAFLD.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Biomedicines<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/biomedicines10102652" target="_blank">https://dx.doi.org/10.3390/biomedicines10102652</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_158e067aebbc82b2ddb693a7de44c975 |
| identifier_str_mv | 10.3390/biomedicines10102652 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25609866 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Investigation of the Effect of Exendin-4 on Oleic Acid-Induced Steatosis in HepG2 Cells Using Fourier Transform Infrared SpectroscopyOlfa Khalifa (10914452)Kamal H. Mroue (18372924)Raghvendra Mall (581171)Ehsan Ullah (2698921)Nayla S. Al-Akl (18372927)Abdelilah Arredouani (10914455)Biological sciencesBiochemistry and cell biologyGeneticsnon-alcoholic fatty liver diseasesteatosisOil Red O stainingFourier transform-infrared spectroscopyprincipal component analysis<div><p>Non-alcoholic fatty liver disease (NAFLD) is a common liver lesion that is untreatable with medications. Glucagon-like peptide-1 receptor (GLP-1R) agonists have recently emerged as a potential NAFLD pharmacotherapy. However, the molecular mechanisms underlying these drugs’ beneficial effects are not fully understood. Using Fourier transform infrared (FTIR) spectroscopy, we sought to investigate the biochemical changes in a steatosis cell model treated or not with the GLP-1R agonist Exendin-4 (Ex-4). HepG2 cells were made steatotic with 400 µM of oleic acid and then treated with 200 nM Ex-4 in order to reduce lipid accumulation. We quantified steatosis using the Oil Red O staining method. We investigated the biochemical alterations induced by steatosis and Ex-4 treatment using Fourier transform infrared (FTIR) spectroscopy and chemometric analyses. Analysis of the Oil Red O staining showed that Ex-4 significantly reduces steatosis. This reduction was confirmed by FTIR analysis, as the phospholipid band (C=O) at 1740 cm−1 in Ex-4 treated cells is significantly decreased compared to steatotic cells. The principal component analysis score plots for both the lipid and protein regions showed that the untreated and Ex-4-treated samples, while still separated, are clustered close to each other, far from the steatotic cells. The biochemical and structural changes induced by OA-induced lipotoxicity are at least partially reversed upon Ex-4 treatment. FTIR and chemometric analyses revealed that Ex-4 significantly reduces OA-induced lipid accumulation, and Ex-4 also restored the lipid and protein biochemical alterations caused by lipotoxicity-induced oxidative stress. In combination with chemometric analyses, FTIR spectroscopy may offer new approaches for investigating the mechanisms underpinning NAFLD.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Biomedicines<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/biomedicines10102652" target="_blank">https://dx.doi.org/10.3390/biomedicines10102652</a></p>2022-10-20T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/biomedicines10102652https://figshare.com/articles/journal_contribution/Investigation_of_the_Effect_of_Exendin-4_on_Oleic_Acid-Induced_Steatosis_in_HepG2_Cells_Using_Fourier_Transform_Infrared_Spectroscopy/25609866CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/256098662022-10-20T03:00:00Z |
| spellingShingle | Investigation of the Effect of Exendin-4 on Oleic Acid-Induced Steatosis in HepG2 Cells Using Fourier Transform Infrared Spectroscopy Olfa Khalifa (10914452) Biological sciences Biochemistry and cell biology Genetics non-alcoholic fatty liver disease steatosis Oil Red O staining Fourier transform-infrared spectroscopy principal component analysis |
| status_str | publishedVersion |
| title | Investigation of the Effect of Exendin-4 on Oleic Acid-Induced Steatosis in HepG2 Cells Using Fourier Transform Infrared Spectroscopy |
| title_full | Investigation of the Effect of Exendin-4 on Oleic Acid-Induced Steatosis in HepG2 Cells Using Fourier Transform Infrared Spectroscopy |
| title_fullStr | Investigation of the Effect of Exendin-4 on Oleic Acid-Induced Steatosis in HepG2 Cells Using Fourier Transform Infrared Spectroscopy |
| title_full_unstemmed | Investigation of the Effect of Exendin-4 on Oleic Acid-Induced Steatosis in HepG2 Cells Using Fourier Transform Infrared Spectroscopy |
| title_short | Investigation of the Effect of Exendin-4 on Oleic Acid-Induced Steatosis in HepG2 Cells Using Fourier Transform Infrared Spectroscopy |
| title_sort | Investigation of the Effect of Exendin-4 on Oleic Acid-Induced Steatosis in HepG2 Cells Using Fourier Transform Infrared Spectroscopy |
| topic | Biological sciences Biochemistry and cell biology Genetics non-alcoholic fatty liver disease steatosis Oil Red O staining Fourier transform-infrared spectroscopy principal component analysis |