Isolation, identification, and potential applications of endophytic bacteria from brown macroalga <i>Hormophysa triquetra</i>
<p dir="ltr">The study aimed at isolation of bacterial endophytes from brown macroalga <i>Hormophysa triquetra</i> (HT). A total of 16 endophytic strains were obtained, belonging to five genera: <i>Bacillus, Pseudomonas, Rothia, Lysinibacillus, </i>and <i&g...
محفوظ في:
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| مؤلفون آخرون: | , , , |
| منشور في: |
2025
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| الموضوعات: | |
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إضافة وسم
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| _version_ | 1864513508253630464 |
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| author | Shazia Bibi (16500318) |
| author2 | Sara Gabr (22963060) Atefeh Esmaeili (462337) Iman Saleh (14152878) Mohammed H. Abu-Dieyeh (14152761) |
| author2_role | author author author author |
| author_facet | Shazia Bibi (16500318) Sara Gabr (22963060) Atefeh Esmaeili (462337) Iman Saleh (14152878) Mohammed H. Abu-Dieyeh (14152761) |
| author_role | author |
| dc.creator.none.fl_str_mv | Shazia Bibi (16500318) Sara Gabr (22963060) Atefeh Esmaeili (462337) Iman Saleh (14152878) Mohammed H. Abu-Dieyeh (14152761) |
| dc.date.none.fl_str_mv | 2025-12-26T18:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.bcab.2025.103901 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Isolation_identification_and_potential_applications_of_endophytic_bacteria_from_brown_macroalga_i_Hormophysa_triquetra_i_/31017352 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Agricultural, veterinary and food sciences Agricultural biotechnology Biological sciences Microbiology Environmental sciences Climate change impacts and adaptation Environmental management Endophytes Plant growth promoters Macroalgae Phytopathogens Biostimulants |
| dc.title.none.fl_str_mv | Isolation, identification, and potential applications of endophytic bacteria from brown macroalga <i>Hormophysa triquetra</i> |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">The study aimed at isolation of bacterial endophytes from brown macroalga <i>Hormophysa triquetra</i> (HT). A total of 16 endophytic strains were obtained, belonging to five genera: <i>Bacillus, Pseudomonas, Rothia, Lysinibacillus, </i>and <i>Stenotrophomonas</i>. The isolates were identified based on 16S rRNA gene sequencing. Multiple assays — including hydrogen cyanide (HCN) production, phosphate solubilization, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, indole acetic acid (IAA) production, and protease enzyme activity were conducted. Moreover, salt tolerance assays and biocontrol tests against <i>Fusarium oxysporum</i> and <i>Botrytis cinerea </i>were also achieved. The results revealed promising plant growth–promoting and stress-mitigating capabilities among the isolated endophytes with strong performance by <u>Bacillus cereus</u> (HT3), which also proved to be effective against fungal strains and showed tolerance to saline conditions. Greenhouse experiments using HT3-treated tomato seedlings further confirmed the in vitro findings. Under abiotic (salinity) and biotic (<i>F. oxysporum</i>) stressed conditions, tomato seedlings treated with HT3 exhibited healthy growth regardless of stressors’ presence. This study highlights the potential of endophytes as biofertilizers and biocontrol agents, offering a promising step toward more sustainable and resilient agricultural practices.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Biocatalysis and Agricultural Biotechnology<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.bcab.2025.103901" target="_blank">https://dx.doi.org/10.1016/j.bcab.2025.103901</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_15bf0e1a533d06c68225b7d80a1c7419 |
| identifier_str_mv | 10.1016/j.bcab.2025.103901 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/31017352 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Isolation, identification, and potential applications of endophytic bacteria from brown macroalga <i>Hormophysa triquetra</i>Shazia Bibi (16500318)Sara Gabr (22963060)Atefeh Esmaeili (462337)Iman Saleh (14152878)Mohammed H. Abu-Dieyeh (14152761)Agricultural, veterinary and food sciencesAgricultural biotechnologyBiological sciencesMicrobiologyEnvironmental sciencesClimate change impacts and adaptationEnvironmental managementEndophytesPlant growth promotersMacroalgaePhytopathogensBiostimulants<p dir="ltr">The study aimed at isolation of bacterial endophytes from brown macroalga <i>Hormophysa triquetra</i> (HT). A total of 16 endophytic strains were obtained, belonging to five genera: <i>Bacillus, Pseudomonas, Rothia, Lysinibacillus, </i>and <i>Stenotrophomonas</i>. The isolates were identified based on 16S rRNA gene sequencing. Multiple assays — including hydrogen cyanide (HCN) production, phosphate solubilization, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, indole acetic acid (IAA) production, and protease enzyme activity were conducted. Moreover, salt tolerance assays and biocontrol tests against <i>Fusarium oxysporum</i> and <i>Botrytis cinerea </i>were also achieved. The results revealed promising plant growth–promoting and stress-mitigating capabilities among the isolated endophytes with strong performance by <u>Bacillus cereus</u> (HT3), which also proved to be effective against fungal strains and showed tolerance to saline conditions. Greenhouse experiments using HT3-treated tomato seedlings further confirmed the in vitro findings. Under abiotic (salinity) and biotic (<i>F. oxysporum</i>) stressed conditions, tomato seedlings treated with HT3 exhibited healthy growth regardless of stressors’ presence. This study highlights the potential of endophytes as biofertilizers and biocontrol agents, offering a promising step toward more sustainable and resilient agricultural practices.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Biocatalysis and Agricultural Biotechnology<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.bcab.2025.103901" target="_blank">https://dx.doi.org/10.1016/j.bcab.2025.103901</a></p>2025-12-26T18:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.bcab.2025.103901https://figshare.com/articles/journal_contribution/Isolation_identification_and_potential_applications_of_endophytic_bacteria_from_brown_macroalga_i_Hormophysa_triquetra_i_/31017352CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/310173522025-12-26T18:00:00Z |
| spellingShingle | Isolation, identification, and potential applications of endophytic bacteria from brown macroalga <i>Hormophysa triquetra</i> Shazia Bibi (16500318) Agricultural, veterinary and food sciences Agricultural biotechnology Biological sciences Microbiology Environmental sciences Climate change impacts and adaptation Environmental management Endophytes Plant growth promoters Macroalgae Phytopathogens Biostimulants |
| status_str | publishedVersion |
| title | Isolation, identification, and potential applications of endophytic bacteria from brown macroalga <i>Hormophysa triquetra</i> |
| title_full | Isolation, identification, and potential applications of endophytic bacteria from brown macroalga <i>Hormophysa triquetra</i> |
| title_fullStr | Isolation, identification, and potential applications of endophytic bacteria from brown macroalga <i>Hormophysa triquetra</i> |
| title_full_unstemmed | Isolation, identification, and potential applications of endophytic bacteria from brown macroalga <i>Hormophysa triquetra</i> |
| title_short | Isolation, identification, and potential applications of endophytic bacteria from brown macroalga <i>Hormophysa triquetra</i> |
| title_sort | Isolation, identification, and potential applications of endophytic bacteria from brown macroalga <i>Hormophysa triquetra</i> |
| topic | Agricultural, veterinary and food sciences Agricultural biotechnology Biological sciences Microbiology Environmental sciences Climate change impacts and adaptation Environmental management Endophytes Plant growth promoters Macroalgae Phytopathogens Biostimulants |