CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering
<p dir="ltr">The identification of the robust clustered regularly interspersed short palindromic repeats (CRISPR) associated endonuclease (Cas9) system gene-editing tool has opened up a wide range of potential therapeutic applications that were restricted by more complex tools, inclu...
محفوظ في:
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| مؤلفون آخرون: | , , |
| منشور في: |
2020
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| _version_ | 1864513512988999680 |
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| author | Maryam Saifaldeen (12561970) |
| author2 | Dana E. Al-Ansari (16327350) Dindial Ramotar (208416) Mustapha Aouida (417652) |
| author2_role | author author author |
| author_facet | Maryam Saifaldeen (12561970) Dana E. Al-Ansari (16327350) Dindial Ramotar (208416) Mustapha Aouida (417652) |
| author_role | author |
| dc.creator.none.fl_str_mv | Maryam Saifaldeen (12561970) Dana E. Al-Ansari (16327350) Dindial Ramotar (208416) Mustapha Aouida (417652) |
| dc.date.none.fl_str_mv | 2020-11-21T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/cells9112518 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/CRISPR_FokI_Dead_Cas9_System_Principles_and_Applications_in_Genome_Engineering/25912249 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Microbiology genome engineering CRISPR dead Cas9 FokI endonuclease FokI-dCas9 RNA-guided FokI nuclease spacer sequence dual guide RNA fdCas9 variants |
| dc.title.none.fl_str_mv | CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">The identification of the robust clustered regularly interspersed short palindromic repeats (CRISPR) associated endonuclease (Cas9) system gene-editing tool has opened up a wide range of potential therapeutic applications that were restricted by more complex tools, including zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). Nevertheless, the high frequency of CRISPR system off-target activity still limits its applications, and, thus, advanced strategies for highly specific CRISPR/Cas9-mediated genome editing are continuously under development including CRISPR–FokI dead Cas9 (fdCas9). fdCas9 system is derived from linking a FokI endonuclease catalytic domain to an inactive Cas9 protein and requires a pair of guide sgRNAs that bind to the sense and antisense strands of the DNA in a protospacer adjacent motif (PAM)-out orientation, with a defined spacer sequence range around the target site. The dimerization of FokI domains generates DNA double-strand breaks, which activates the DNA repair machinery and results in genomic edit. So far, all the engineered fdCas9 variants have shown promising gene-editing activities in human cells when compared to other platforms. Herein, we review the advantages of all published variants of fdCas9 and their current applications in genome engineering.</p><h2>Other Information</h2><p dir="ltr">Published in: Cells<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/cells9112518" target="_blank">https://dx.doi.org/10.3390/cells9112518</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_183fcf1c2ec97a7deae9fa43566a7173 |
| identifier_str_mv | 10.3390/cells9112518 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25912249 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | CRISPR FokI Dead Cas9 System: Principles and Applications in Genome EngineeringMaryam Saifaldeen (12561970)Dana E. Al-Ansari (16327350)Dindial Ramotar (208416)Mustapha Aouida (417652)Biological sciencesGeneticsMicrobiologygenome engineeringCRISPRdead Cas9FokI endonucleaseFokI-dCas9RNA-guided FokI nucleasespacer sequencedual guide RNAfdCas9 variants<p dir="ltr">The identification of the robust clustered regularly interspersed short palindromic repeats (CRISPR) associated endonuclease (Cas9) system gene-editing tool has opened up a wide range of potential therapeutic applications that were restricted by more complex tools, including zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). Nevertheless, the high frequency of CRISPR system off-target activity still limits its applications, and, thus, advanced strategies for highly specific CRISPR/Cas9-mediated genome editing are continuously under development including CRISPR–FokI dead Cas9 (fdCas9). fdCas9 system is derived from linking a FokI endonuclease catalytic domain to an inactive Cas9 protein and requires a pair of guide sgRNAs that bind to the sense and antisense strands of the DNA in a protospacer adjacent motif (PAM)-out orientation, with a defined spacer sequence range around the target site. The dimerization of FokI domains generates DNA double-strand breaks, which activates the DNA repair machinery and results in genomic edit. So far, all the engineered fdCas9 variants have shown promising gene-editing activities in human cells when compared to other platforms. Herein, we review the advantages of all published variants of fdCas9 and their current applications in genome engineering.</p><h2>Other Information</h2><p dir="ltr">Published in: Cells<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/cells9112518" target="_blank">https://dx.doi.org/10.3390/cells9112518</a></p>2020-11-21T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/cells9112518https://figshare.com/articles/journal_contribution/CRISPR_FokI_Dead_Cas9_System_Principles_and_Applications_in_Genome_Engineering/25912249CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/259122492020-11-21T09:00:00Z |
| spellingShingle | CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering Maryam Saifaldeen (12561970) Biological sciences Genetics Microbiology genome engineering CRISPR dead Cas9 FokI endonuclease FokI-dCas9 RNA-guided FokI nuclease spacer sequence dual guide RNA fdCas9 variants |
| status_str | publishedVersion |
| title | CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering |
| title_full | CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering |
| title_fullStr | CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering |
| title_full_unstemmed | CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering |
| title_short | CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering |
| title_sort | CRISPR FokI Dead Cas9 System: Principles and Applications in Genome Engineering |
| topic | Biological sciences Genetics Microbiology genome engineering CRISPR dead Cas9 FokI endonuclease FokI-dCas9 RNA-guided FokI nuclease spacer sequence dual guide RNA fdCas9 variants |