Where the infection is isolated rather than the specific species correlates with adherence strength, whereas biofilm density remains static in clinically isolated <i>Candida</i> and arthroconidial yeasts

<p dir="ltr">To colonize and infect the host, arthroconidial yeasts must avoid being killed by the host’s defenses. The formation of biofilms on implanted devices allows fungi to avoid host responses and to disseminate into the host. To better study the mechanisms of infection by art...

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Main Author: Mei ElGindi (23276131) (author)
Other Authors: Rula Al-Baghdadi (23276134) (author), Alex B. Jackman (20379279) (author), Angelina S. Antonyan (23276137) (author), Diana L. McMahon (23276140) (author), Saad J. Taj-Aldeen (14650087) (author), Jonathan S. Finkel (23276143) (author)
Published: 2021
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author Mei ElGindi (23276131)
author2 Rula Al-Baghdadi (23276134)
Alex B. Jackman (20379279)
Angelina S. Antonyan (23276137)
Diana L. McMahon (23276140)
Saad J. Taj-Aldeen (14650087)
Jonathan S. Finkel (23276143)
author2_role author
author
author
author
author
author
author_facet Mei ElGindi (23276131)
Rula Al-Baghdadi (23276134)
Alex B. Jackman (20379279)
Angelina S. Antonyan (23276137)
Diana L. McMahon (23276140)
Saad J. Taj-Aldeen (14650087)
Jonathan S. Finkel (23276143)
author_role author
dc.creator.none.fl_str_mv Mei ElGindi (23276131)
Rula Al-Baghdadi (23276134)
Alex B. Jackman (20379279)
Angelina S. Antonyan (23276137)
Diana L. McMahon (23276140)
Saad J. Taj-Aldeen (14650087)
Jonathan S. Finkel (23276143)
dc.date.none.fl_str_mv 2021-07-01T00:00:00Z
dc.identifier.none.fl_str_mv 10.1139/cjm-2020-0215
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Where_the_infection_is_isolated_rather_than_the_specific_species_correlates_with_adherence_strength_whereas_biofilm_density_remains_static_in_clinically_isolated_i_Candida_i_and_arthroconidial_yeasts/31444705
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biological sciences
Microbiology
Biomedical and clinical sciences
Immunology
Pharmacology and pharmaceutical sciences
Health sciences
Epidemiology
Public health
adherence
biofilm
Candida
Trichosporon
Magnusiomyces
dc.title.none.fl_str_mv Where the infection is isolated rather than the specific species correlates with adherence strength, whereas biofilm density remains static in clinically isolated <i>Candida</i> and arthroconidial yeasts
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">To colonize and infect the host, arthroconidial yeasts must avoid being killed by the host’s defenses. The formation of biofilms on implanted devices allows fungi to avoid host responses and to disseminate into the host. To better study the mechanisms of infection by arthroconidial yeasts, adherence and biofilm formation were assayed using patient samples collected over 10 years. In clinical samples, adherence varies within species, but the relative adherence is constant for those samples isolated from the same infection site. Herein we document, for the first time, in-vitro biofilm formation by <i>Trichosporon dohaense, T. ovoides, T. japonicum, T. coremiiforme, Cutaneotrichosporon mucoides, Cutaneotrichosporon cutaneum, Galactomyces candidus, </i>and<i> Magnusiomyces capitatus</i> on clinically relevant catheter material. Analysis of biofilm biomass assays indicated that biofilm mass changes less than 2-fold, regardless of the species. Our results support the hypothesis that most pathogenic fungi can form biofilms, and that biofilm formation is a source of systemic infections.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Canadian Journal of Microbiology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/deed.en" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1139/cjm-2020-0215" target="_blank">https://dx.doi.org/10.1139/cjm-2020-0215</a></p>
eu_rights_str_mv openAccess
id Manara2_1e593a257390c66c0331e5c4a517eb52
identifier_str_mv 10.1139/cjm-2020-0215
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/31444705
publishDate 2021
repository.mail.fl_str_mv
repository.name.fl_str_mv
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rights_invalid_str_mv CC BY 4.0
spelling Where the infection is isolated rather than the specific species correlates with adherence strength, whereas biofilm density remains static in clinically isolated <i>Candida</i> and arthroconidial yeastsMei ElGindi (23276131)Rula Al-Baghdadi (23276134)Alex B. Jackman (20379279)Angelina S. Antonyan (23276137)Diana L. McMahon (23276140)Saad J. Taj-Aldeen (14650087)Jonathan S. Finkel (23276143)Biological sciencesMicrobiologyBiomedical and clinical sciencesImmunologyPharmacology and pharmaceutical sciencesHealth sciencesEpidemiologyPublic healthadherencebiofilmCandidaTrichosporonMagnusiomyces<p dir="ltr">To colonize and infect the host, arthroconidial yeasts must avoid being killed by the host’s defenses. The formation of biofilms on implanted devices allows fungi to avoid host responses and to disseminate into the host. To better study the mechanisms of infection by arthroconidial yeasts, adherence and biofilm formation were assayed using patient samples collected over 10 years. In clinical samples, adherence varies within species, but the relative adherence is constant for those samples isolated from the same infection site. Herein we document, for the first time, in-vitro biofilm formation by <i>Trichosporon dohaense, T. ovoides, T. japonicum, T. coremiiforme, Cutaneotrichosporon mucoides, Cutaneotrichosporon cutaneum, Galactomyces candidus, </i>and<i> Magnusiomyces capitatus</i> on clinically relevant catheter material. Analysis of biofilm biomass assays indicated that biofilm mass changes less than 2-fold, regardless of the species. Our results support the hypothesis that most pathogenic fungi can form biofilms, and that biofilm formation is a source of systemic infections.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Canadian Journal of Microbiology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/deed.en" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1139/cjm-2020-0215" target="_blank">https://dx.doi.org/10.1139/cjm-2020-0215</a></p>2021-07-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1139/cjm-2020-0215https://figshare.com/articles/journal_contribution/Where_the_infection_is_isolated_rather_than_the_specific_species_correlates_with_adherence_strength_whereas_biofilm_density_remains_static_in_clinically_isolated_i_Candida_i_and_arthroconidial_yeasts/31444705CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/314447052021-07-01T00:00:00Z
spellingShingle Where the infection is isolated rather than the specific species correlates with adherence strength, whereas biofilm density remains static in clinically isolated <i>Candida</i> and arthroconidial yeasts
Mei ElGindi (23276131)
Biological sciences
Microbiology
Biomedical and clinical sciences
Immunology
Pharmacology and pharmaceutical sciences
Health sciences
Epidemiology
Public health
adherence
biofilm
Candida
Trichosporon
Magnusiomyces
status_str publishedVersion
title Where the infection is isolated rather than the specific species correlates with adherence strength, whereas biofilm density remains static in clinically isolated <i>Candida</i> and arthroconidial yeasts
title_full Where the infection is isolated rather than the specific species correlates with adherence strength, whereas biofilm density remains static in clinically isolated <i>Candida</i> and arthroconidial yeasts
title_fullStr Where the infection is isolated rather than the specific species correlates with adherence strength, whereas biofilm density remains static in clinically isolated <i>Candida</i> and arthroconidial yeasts
title_full_unstemmed Where the infection is isolated rather than the specific species correlates with adherence strength, whereas biofilm density remains static in clinically isolated <i>Candida</i> and arthroconidial yeasts
title_short Where the infection is isolated rather than the specific species correlates with adherence strength, whereas biofilm density remains static in clinically isolated <i>Candida</i> and arthroconidial yeasts
title_sort Where the infection is isolated rather than the specific species correlates with adherence strength, whereas biofilm density remains static in clinically isolated <i>Candida</i> and arthroconidial yeasts
topic Biological sciences
Microbiology
Biomedical and clinical sciences
Immunology
Pharmacology and pharmaceutical sciences
Health sciences
Epidemiology
Public health
adherence
biofilm
Candida
Trichosporon
Magnusiomyces