Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacity

<h3>Purpose</h3><p dir="ltr">Different strategies for near-infrared spectroscopy (NIRS)-derived muscle oxidative capacity assessment have been reported. This study compared and evaluated (I) approaches for averaging trials; (II) NIRS signals and blood volume correction eq...

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Main Author: Letizia Rasica (19256467) (author)
Other Authors: Erin Calaine Inglis (7042334) (author), Raffaele Mazzolari (19256470) (author), Danilo Iannetta (7042337) (author), Juan M. Murias (19256473) (author)
Published: 2024
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_version_ 1864513509812862976
author Letizia Rasica (19256467)
author2 Erin Calaine Inglis (7042334)
Raffaele Mazzolari (19256470)
Danilo Iannetta (7042337)
Juan M. Murias (19256473)
author2_role author
author
author
author
author_facet Letizia Rasica (19256467)
Erin Calaine Inglis (7042334)
Raffaele Mazzolari (19256470)
Danilo Iannetta (7042337)
Juan M. Murias (19256473)
author_role author
dc.creator.none.fl_str_mv Letizia Rasica (19256467)
Erin Calaine Inglis (7042334)
Raffaele Mazzolari (19256470)
Danilo Iannetta (7042337)
Juan M. Murias (19256473)
dc.date.none.fl_str_mv 2024-02-24T09:00:00Z
dc.identifier.none.fl_str_mv 10.1007/s00421-024-05421-6
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Methodological_considerations_on_near-infrared_spectroscopy_derived_muscle_oxidative_capacity/26403994
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biological sciences
Biochemistry and cell biology
Health sciences
Sports science and exercise
NIRS
Skeletal muscle oxidative metabolism
Fitness level
Vastus lateralis
Tibialis anterior
dc.title.none.fl_str_mv Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacity
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <h3>Purpose</h3><p dir="ltr">Different strategies for near-infrared spectroscopy (NIRS)-derived muscle oxidative capacity assessment have been reported. This study compared and evaluated (I) approaches for averaging trials; (II) NIRS signals and blood volume correction equations; (III) the assessment of vastus lateralis (VL) and tibialis anterior (TA) muscles in two fitness levels groups. </p><h3>Methods </h3><p dir="ltr">Thirty-six participants [18 chronically trained (CT: 14 males, 4 females) and 18 untrained (UT: 10 males, 8 females)] participated in this study. Two trials of twenty transient arterial occlusions were performed for NIRS-derived muscle oxidative capacity assessment. Muscle oxygen consumption (<i>V</i>˙O<sub>2</sub>m) was estimated from deoxygenated hemoglobin (HHb), corrected for blood volume changes following Ryan (HHbR) and Beever (HHbB) equations, and from oxygen saturation (StO<sub>2</sub>) in VL and TA. </p><h3>Results </h3><p dir="ltr">Superimposing or averaging <i>V</i>˙O<sub>2</sub>m or averaging the rate constants (<i>k</i>) from the two trials resulted in equivalent k values [two one-sided tests (TOST) procedure with 5% equivalence margin—<i>P</i> < 0.001]. Whereas HHbR (2.35 ± 0.61 min−<sup>1</sup>) and HHbB (2.34 ± 0.58 min<sup>−1</sup>) derived k were equivalent (P < 0.001), StO2 derived k (2.81 ± 0.92 min−1) was greater (P < 0.001) than both. <i>k </i>values were greater in CT vs UT in both muscles (VL: + 0.68 min−<sup>1</sup>, <i>P</i> = 0.002; TA: + 0.43 min−1, <i>P</i> = 0.01). </p><h3>Conclusion </h3><p dir="ltr">Different approaches for averaging trials lead to similar k. HHb and StO2 signals provided different k, although different blood volume corrections did not impact k. Group differences in k were detected in both muscles.</p><h2>Other Information</h2><p dir="ltr">Published in: European Journal of Applied Physiology<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s00421-024-05421-6" target="_blank">https://dx.doi.org/10.1007/s00421-024-05421-6</a></p>
eu_rights_str_mv openAccess
id Manara2_202fcf109609821f67ff818374f6f68c
identifier_str_mv 10.1007/s00421-024-05421-6
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/26403994
publishDate 2024
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rights_invalid_str_mv CC BY 4.0
spelling Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacityLetizia Rasica (19256467)Erin Calaine Inglis (7042334)Raffaele Mazzolari (19256470)Danilo Iannetta (7042337)Juan M. Murias (19256473)Biological sciencesBiochemistry and cell biologyHealth sciencesSports science and exerciseNIRSSkeletal muscle oxidative metabolismFitness levelVastus lateralisTibialis anterior<h3>Purpose</h3><p dir="ltr">Different strategies for near-infrared spectroscopy (NIRS)-derived muscle oxidative capacity assessment have been reported. This study compared and evaluated (I) approaches for averaging trials; (II) NIRS signals and blood volume correction equations; (III) the assessment of vastus lateralis (VL) and tibialis anterior (TA) muscles in two fitness levels groups. </p><h3>Methods </h3><p dir="ltr">Thirty-six participants [18 chronically trained (CT: 14 males, 4 females) and 18 untrained (UT: 10 males, 8 females)] participated in this study. Two trials of twenty transient arterial occlusions were performed for NIRS-derived muscle oxidative capacity assessment. Muscle oxygen consumption (<i>V</i>˙O<sub>2</sub>m) was estimated from deoxygenated hemoglobin (HHb), corrected for blood volume changes following Ryan (HHbR) and Beever (HHbB) equations, and from oxygen saturation (StO<sub>2</sub>) in VL and TA. </p><h3>Results </h3><p dir="ltr">Superimposing or averaging <i>V</i>˙O<sub>2</sub>m or averaging the rate constants (<i>k</i>) from the two trials resulted in equivalent k values [two one-sided tests (TOST) procedure with 5% equivalence margin—<i>P</i> < 0.001]. Whereas HHbR (2.35 ± 0.61 min−<sup>1</sup>) and HHbB (2.34 ± 0.58 min<sup>−1</sup>) derived k were equivalent (P < 0.001), StO2 derived k (2.81 ± 0.92 min−1) was greater (P < 0.001) than both. <i>k </i>values were greater in CT vs UT in both muscles (VL: + 0.68 min−<sup>1</sup>, <i>P</i> = 0.002; TA: + 0.43 min−1, <i>P</i> = 0.01). </p><h3>Conclusion </h3><p dir="ltr">Different approaches for averaging trials lead to similar k. HHb and StO2 signals provided different k, although different blood volume corrections did not impact k. Group differences in k were detected in both muscles.</p><h2>Other Information</h2><p dir="ltr">Published in: European Journal of Applied Physiology<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s00421-024-05421-6" target="_blank">https://dx.doi.org/10.1007/s00421-024-05421-6</a></p>2024-02-24T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s00421-024-05421-6https://figshare.com/articles/journal_contribution/Methodological_considerations_on_near-infrared_spectroscopy_derived_muscle_oxidative_capacity/26403994CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/264039942024-02-24T09:00:00Z
spellingShingle Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacity
Letizia Rasica (19256467)
Biological sciences
Biochemistry and cell biology
Health sciences
Sports science and exercise
NIRS
Skeletal muscle oxidative metabolism
Fitness level
Vastus lateralis
Tibialis anterior
status_str publishedVersion
title Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacity
title_full Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacity
title_fullStr Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacity
title_full_unstemmed Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacity
title_short Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacity
title_sort Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacity
topic Biological sciences
Biochemistry and cell biology
Health sciences
Sports science and exercise
NIRS
Skeletal muscle oxidative metabolism
Fitness level
Vastus lateralis
Tibialis anterior