Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions
<p>A growing interest has recently emerged in the use of nanomaterials in medical applications. Nanomaterials, such as MXene, have unique properties due to their 2D ultra-thin structure, which is potentially useful in cancer photothermal therapy. To be most effective, photothermal agents need...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , |
| Published: |
2021
|
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1864513529023823872 |
|---|---|
| author | Samar Shurbaji (9938696) |
| author2 | Nimshitha P. Abdul Manaph (16442016) Samia M. Ltaief (16078859) Abeer R. Al-Shammari (16078952) Ahmed Elzatahry (1515655) Huseyin C. Yalcin (16442020) |
| author2_role | author author author author author |
| author_facet | Samar Shurbaji (9938696) Nimshitha P. Abdul Manaph (16442016) Samia M. Ltaief (16078859) Abeer R. Al-Shammari (16078952) Ahmed Elzatahry (1515655) Huseyin C. Yalcin (16442020) |
| author_role | author |
| dc.creator.none.fl_str_mv | Samar Shurbaji (9938696) Nimshitha P. Abdul Manaph (16442016) Samia M. Ltaief (16078859) Abeer R. Al-Shammari (16078952) Ahmed Elzatahry (1515655) Huseyin C. Yalcin (16442020) |
| dc.date.none.fl_str_mv | 2021-09-08T06:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fnano.2021.689718 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Characterization_of_MXene_as_a_Cancer_Photothermal_Agent_Under_Physiological_Conditions/23575860 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Oncology and carcinogenesis Engineering Biomedical engineering Nanotechnology Cancer photothermal therapy static dynamic MXene |
| dc.title.none.fl_str_mv | Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>A growing interest has recently emerged in the use of nanomaterials in medical applications. Nanomaterials, such as MXene, have unique properties due to their 2D ultra-thin structure, which is potentially useful in cancer photothermal therapy. To be most effective, photothermal agents need to be internalized by the cancer cells. In this study, MXene was fabricated using chemical reactions and tested as a photothermal agent on MDA-231 breast cancer cells under static and physiological conditions. Fluid shear stress (∼0.1 Dyn/cm<sup>2</sup>) was applied using a perfusion system to mimic the physiological tumor microenvironment. The uptake of MXene was analyzed under fluid flow compared to static culture using confocal microscopy, scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), and transmission electron microscopy (TEM). Furthermore, a viability assay was used to assess cell’s survival after exposing the treated cells to photothermal laser at different power densities and durations. We showed that when incubated with cancer cells, 2D MXene nanoparticles were successfully internalized into the cells resulting in increased intracellular temperatures when exposed to NIR laser. Interestingly, dynamic culture alone did not result in a significant increase in uptake suggesting the need for surface modifications for enhanced cellular uptake under shear stress. </p> <h2>Other Information</h2> <p>Published in: Frontiers in Nanotechnology<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.3389/fnano.2021.689718" target="_blank">http://dx.doi.org/10.3389/fnano.2021.689718 </a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_21f13d9f6152a92995e8b674103488bc |
| identifier_str_mv | 10.3389/fnano.2021.689718 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/23575860 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Characterization of MXene as a Cancer Photothermal Agent Under Physiological ConditionsSamar Shurbaji (9938696)Nimshitha P. Abdul Manaph (16442016)Samia M. Ltaief (16078859)Abeer R. Al-Shammari (16078952)Ahmed Elzatahry (1515655)Huseyin C. Yalcin (16442020)Biomedical and clinical sciencesOncology and carcinogenesisEngineeringBiomedical engineeringNanotechnologyCancerphotothermal therapystaticdynamicMXene<p>A growing interest has recently emerged in the use of nanomaterials in medical applications. Nanomaterials, such as MXene, have unique properties due to their 2D ultra-thin structure, which is potentially useful in cancer photothermal therapy. To be most effective, photothermal agents need to be internalized by the cancer cells. In this study, MXene was fabricated using chemical reactions and tested as a photothermal agent on MDA-231 breast cancer cells under static and physiological conditions. Fluid shear stress (∼0.1 Dyn/cm<sup>2</sup>) was applied using a perfusion system to mimic the physiological tumor microenvironment. The uptake of MXene was analyzed under fluid flow compared to static culture using confocal microscopy, scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), and transmission electron microscopy (TEM). Furthermore, a viability assay was used to assess cell’s survival after exposing the treated cells to photothermal laser at different power densities and durations. We showed that when incubated with cancer cells, 2D MXene nanoparticles were successfully internalized into the cells resulting in increased intracellular temperatures when exposed to NIR laser. Interestingly, dynamic culture alone did not result in a significant increase in uptake suggesting the need for surface modifications for enhanced cellular uptake under shear stress. </p> <h2>Other Information</h2> <p>Published in: Frontiers in Nanotechnology<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.3389/fnano.2021.689718" target="_blank">http://dx.doi.org/10.3389/fnano.2021.689718 </a></p>2021-09-08T06:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fnano.2021.689718https://figshare.com/articles/journal_contribution/Characterization_of_MXene_as_a_Cancer_Photothermal_Agent_Under_Physiological_Conditions/23575860CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/235758602021-09-08T06:00:00Z |
| spellingShingle | Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions Samar Shurbaji (9938696) Biomedical and clinical sciences Oncology and carcinogenesis Engineering Biomedical engineering Nanotechnology Cancer photothermal therapy static dynamic MXene |
| status_str | publishedVersion |
| title | Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions |
| title_full | Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions |
| title_fullStr | Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions |
| title_full_unstemmed | Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions |
| title_short | Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions |
| title_sort | Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions |
| topic | Biomedical and clinical sciences Oncology and carcinogenesis Engineering Biomedical engineering Nanotechnology Cancer photothermal therapy static dynamic MXene |