Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteria
<p dir="ltr">Bacterial infection poses a significant concern and represents a global threat. The misuse of <u>antibacterial drugs</u> causes resistance, often multi-resistance, of bacteria to one or more of these antibiotics. New approaches are needed to overcome such lim...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , |
| Published: |
2025
|
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <p dir="ltr">Bacterial infection poses a significant concern and represents a global threat. The misuse of <u>antibacterial drugs</u> causes resistance, often multi-resistance, of bacteria to one or more of these antibiotics. New approaches are needed to overcome such limitations of the antibiotic therapy. The copper based <u>metal organic framework</u> (Cu-MOFs) showed an extraordinary antibacterial effectiveness and can be considered as broad-spectrum <u>antibacterial agents.</u> This study focuses on investigating the specific chemical and physical characteristics of Cu-MOFs, investigating their antibacterial capabilities on three representative bacterial strains, and linking their structure to their function. Several techniques for characterization were employed. Analysis through<u> Fourier Transform Infrared Spectroscopy</u> (FTIR) of untreated and Cu-MOF-treated bacterial cells revealed distinct features indicating surface modifications of bacterial cells due to Cu-MOF interaction. The crystalline structure of synthesized Cu-MOFs was validated using X-ray diffraction (XRD) analysis, exhibiting peaks at 14.7° and 24.3°. X-ray photoelectron spectroscopy (XPS) was used for the analysis of the Cu-MOFs showing characteristic peaks confirming their <u>elemental composition</u> (Cu, C, N, O, S). <u>Transmission Electron Microscopy</u> (TEM) analysis verified the significant <u>biocidal</u> activity of Cu-MOFs against both the Gram-negative (<i><u>Escherichia coli</u></i>) and Gram-positive (<u>Staphylococcus aureus</u> and <i>Bacillus subtilis</i>) bacteria, by disrupting their cell membranes and inducing cell death primarily through Cu<sup>2+</sup> <u>ion release</u>. The findings demonstrated the role and mechanisms of synthesized Cu-MOFs as broad-spectrum antibacterial agents. However, their structure is shown to be more potent against the Gram-positive bacteria, which keep their shape, although they accumulate the Cu-MOFs intracellularly. The Cu-MOFs <u>nanoparticles</u> entered the cell of the Gram-negative cells of<i> E. coli</i> and caused a clear change in the form, from rod to swollen-circular. Overall, the study highlights the broad-spectrum potential of Cu-MOFs, making them strong candidates for developing active antimicrobial agents in the future, such as antibiotics.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of the Indian Chemical Society<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.jics.2025.101738" target="_blank">https://dx.doi.org/10.1016/j.jics.2025.101738</a></p> |
|---|