Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteria
<p dir="ltr">Bacterial infection poses a significant concern and represents a global threat. The misuse of <u>antibacterial drugs</u> causes resistance, often multi-resistance, of bacteria to one or more of these antibiotics. New approaches are needed to overcome such lim...
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2025
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| _version_ | 1864513532005974016 |
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| author | Muhammad Hubab (20639153) |
| author2 | Nabil Zouari (9193418) Mohammad A. Al-Ghouti (8882054) |
| author2_role | author author |
| author_facet | Muhammad Hubab (20639153) Nabil Zouari (9193418) Mohammad A. Al-Ghouti (8882054) |
| author_role | author |
| dc.creator.none.fl_str_mv | Muhammad Hubab (20639153) Nabil Zouari (9193418) Mohammad A. Al-Ghouti (8882054) |
| dc.date.none.fl_str_mv | 2025-04-29T12:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.jics.2025.101738 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Structure-activity_relationships_of_copper-based_metal-organic_Frameworks_Investigating_antibacterial_mechanisms_against_gram-positive_and_gram-negative_bacteria/30820058 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Microbiology Engineering Materials engineering Cu-MOFs Escherichia coli Staphylococcus aureus Bacillus Antibacterial Material characterization |
| dc.title.none.fl_str_mv | Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteria |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Bacterial infection poses a significant concern and represents a global threat. The misuse of <u>antibacterial drugs</u> causes resistance, often multi-resistance, of bacteria to one or more of these antibiotics. New approaches are needed to overcome such limitations of the antibiotic therapy. The copper based <u>metal organic framework</u> (Cu-MOFs) showed an extraordinary antibacterial effectiveness and can be considered as broad-spectrum <u>antibacterial agents.</u> This study focuses on investigating the specific chemical and physical characteristics of Cu-MOFs, investigating their antibacterial capabilities on three representative bacterial strains, and linking their structure to their function. Several techniques for characterization were employed. Analysis through<u> Fourier Transform Infrared Spectroscopy</u> (FTIR) of untreated and Cu-MOF-treated bacterial cells revealed distinct features indicating surface modifications of bacterial cells due to Cu-MOF interaction. The crystalline structure of synthesized Cu-MOFs was validated using X-ray diffraction (XRD) analysis, exhibiting peaks at 14.7° and 24.3°. X-ray photoelectron spectroscopy (XPS) was used for the analysis of the Cu-MOFs showing characteristic peaks confirming their <u>elemental composition</u> (Cu, C, N, O, S). <u>Transmission Electron Microscopy</u> (TEM) analysis verified the significant <u>biocidal</u> activity of Cu-MOFs against both the Gram-negative (<i><u>Escherichia coli</u></i>) and Gram-positive (<u>Staphylococcus aureus</u> and <i>Bacillus subtilis</i>) bacteria, by disrupting their cell membranes and inducing cell death primarily through Cu<sup>2+</sup> <u>ion release</u>. The findings demonstrated the role and mechanisms of synthesized Cu-MOFs as broad-spectrum antibacterial agents. However, their structure is shown to be more potent against the Gram-positive bacteria, which keep their shape, although they accumulate the Cu-MOFs intracellularly. The Cu-MOFs <u>nanoparticles</u> entered the cell of the Gram-negative cells of<i> E. coli</i> and caused a clear change in the form, from rod to swollen-circular. Overall, the study highlights the broad-spectrum potential of Cu-MOFs, making them strong candidates for developing active antimicrobial agents in the future, such as antibiotics.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of the Indian Chemical Society<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.jics.2025.101738" target="_blank">https://dx.doi.org/10.1016/j.jics.2025.101738</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_22135db733a3bbbb3c589385a847308c |
| identifier_str_mv | 10.1016/j.jics.2025.101738 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/30820058 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteriaMuhammad Hubab (20639153)Nabil Zouari (9193418)Mohammad A. Al-Ghouti (8882054)Biological sciencesMicrobiologyEngineeringMaterials engineeringCu-MOFsEscherichia coliStaphylococcus aureusBacillusAntibacterialMaterial characterization<p dir="ltr">Bacterial infection poses a significant concern and represents a global threat. The misuse of <u>antibacterial drugs</u> causes resistance, often multi-resistance, of bacteria to one or more of these antibiotics. New approaches are needed to overcome such limitations of the antibiotic therapy. The copper based <u>metal organic framework</u> (Cu-MOFs) showed an extraordinary antibacterial effectiveness and can be considered as broad-spectrum <u>antibacterial agents.</u> This study focuses on investigating the specific chemical and physical characteristics of Cu-MOFs, investigating their antibacterial capabilities on three representative bacterial strains, and linking their structure to their function. Several techniques for characterization were employed. Analysis through<u> Fourier Transform Infrared Spectroscopy</u> (FTIR) of untreated and Cu-MOF-treated bacterial cells revealed distinct features indicating surface modifications of bacterial cells due to Cu-MOF interaction. The crystalline structure of synthesized Cu-MOFs was validated using X-ray diffraction (XRD) analysis, exhibiting peaks at 14.7° and 24.3°. X-ray photoelectron spectroscopy (XPS) was used for the analysis of the Cu-MOFs showing characteristic peaks confirming their <u>elemental composition</u> (Cu, C, N, O, S). <u>Transmission Electron Microscopy</u> (TEM) analysis verified the significant <u>biocidal</u> activity of Cu-MOFs against both the Gram-negative (<i><u>Escherichia coli</u></i>) and Gram-positive (<u>Staphylococcus aureus</u> and <i>Bacillus subtilis</i>) bacteria, by disrupting their cell membranes and inducing cell death primarily through Cu<sup>2+</sup> <u>ion release</u>. The findings demonstrated the role and mechanisms of synthesized Cu-MOFs as broad-spectrum antibacterial agents. However, their structure is shown to be more potent against the Gram-positive bacteria, which keep their shape, although they accumulate the Cu-MOFs intracellularly. The Cu-MOFs <u>nanoparticles</u> entered the cell of the Gram-negative cells of<i> E. coli</i> and caused a clear change in the form, from rod to swollen-circular. Overall, the study highlights the broad-spectrum potential of Cu-MOFs, making them strong candidates for developing active antimicrobial agents in the future, such as antibiotics.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of the Indian Chemical Society<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.jics.2025.101738" target="_blank">https://dx.doi.org/10.1016/j.jics.2025.101738</a></p>2025-04-29T12:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.jics.2025.101738https://figshare.com/articles/journal_contribution/Structure-activity_relationships_of_copper-based_metal-organic_Frameworks_Investigating_antibacterial_mechanisms_against_gram-positive_and_gram-negative_bacteria/30820058CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/308200582025-04-29T12:00:00Z |
| spellingShingle | Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteria Muhammad Hubab (20639153) Biological sciences Microbiology Engineering Materials engineering Cu-MOFs Escherichia coli Staphylococcus aureus Bacillus Antibacterial Material characterization |
| status_str | publishedVersion |
| title | Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteria |
| title_full | Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteria |
| title_fullStr | Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteria |
| title_full_unstemmed | Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteria |
| title_short | Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteria |
| title_sort | Structure-activity relationships of copper-based metal-organic Frameworks: Investigating antibacterial mechanisms against gram-positive and gram-negative bacteria |
| topic | Biological sciences Microbiology Engineering Materials engineering Cu-MOFs Escherichia coli Staphylococcus aureus Bacillus Antibacterial Material characterization |