Human leukocyte antigen class II gene diversity tunes antibody repertoires to common pathogens
<div><p>Allelic diversity of human leukocyte antigen (HLA) class II genes may help maintain humoral immunity against infectious diseases. In this study, we investigated germline genetic variation in classical HLA class II genes and employed a systematic, unbiased approach to explore the...
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2022
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| _version_ | 1864513519120023552 |
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| author | Taushif Khan (691254) |
| author2 | Mahbuba Rahman (198499) Ikhlak Ahmed (150200) Fatima Al Ali (13210926) Puthen Veettil Jithesh (12040358) Nico Marr (349853) |
| author2_role | author author author author author |
| author_facet | Taushif Khan (691254) Mahbuba Rahman (198499) Ikhlak Ahmed (150200) Fatima Al Ali (13210926) Puthen Veettil Jithesh (12040358) Nico Marr (349853) |
| author_role | author |
| dc.creator.none.fl_str_mv | Taushif Khan (691254) Mahbuba Rahman (198499) Ikhlak Ahmed (150200) Fatima Al Ali (13210926) Puthen Veettil Jithesh (12040358) Nico Marr (349853) |
| dc.date.none.fl_str_mv | 2022-08-08T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fimmu.2022.856497 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Human_leukocyte_antigen_class_II_gene_diversity_tunes_antibody_repertoires_to_common_pathogens/25658877 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Immunology human antibody repertoires microbial infection phage immunoprecipitation sequencing human leukocyte antigen major histocompatibility complex polymorphisms allelic diversity association study |
| dc.title.none.fl_str_mv | Human leukocyte antigen class II gene diversity tunes antibody repertoires to common pathogens |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>Allelic diversity of human leukocyte antigen (HLA) class II genes may help maintain humoral immunity against infectious diseases. In this study, we investigated germline genetic variation in classical HLA class II genes and employed a systematic, unbiased approach to explore the relative contribution of this genetic variation in the antibody repertoire to various common pathogens. We leveraged a well-defined cohort of 800 adults representing the general Arab population in which genetic material is shared because of the high frequency of consanguineous unions. By applying a high-throughput method for large-scale antibody profiling to this well-defined cohort, we were able to dissect the overall effect of zygosity for classical HLA class II genes, as well as the effects associated with specific HLA class II alleles, haplotypes and genotypes, on the antimicrobial antibody repertoire breadth and antibody specificity with unprecedented resolution. Our population genetic studies revealed that zygosity of the classical HLA class II genes is a strong predictor of antibody responses to common human pathogens, suggesting that classical HLA class II gene heterozygosity confers a selective advantage. Moreover, we demonstrated that multiple HLA class II alleles can have additive effects on the antibody repertoire to common pathogens. We also identified associations of HLA-DRB1 genotypes with specific antigens. Our findings suggest that HLA class II gene polymorphisms confer specific humoral immunity against common pathogens, which may have contributed to the genetic diversity of HLA class II loci during hominine evolution.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Frontiers in Immunology<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fimmu.2022.856497" target="_blank">https://dx.doi.org/10.3389/fimmu.2022.856497</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_2ad9b6b1b632b336498b11e3f83ea812 |
| identifier_str_mv | 10.3389/fimmu.2022.856497 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25658877 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Human leukocyte antigen class II gene diversity tunes antibody repertoires to common pathogensTaushif Khan (691254)Mahbuba Rahman (198499)Ikhlak Ahmed (150200)Fatima Al Ali (13210926)Puthen Veettil Jithesh (12040358)Nico Marr (349853)Biomedical and clinical sciencesImmunologyhuman antibody repertoiresmicrobial infectionphage immunoprecipitation sequencinghuman leukocyte antigenmajor histocompatibility complexpolymorphismsallelic diversityassociation study<div><p>Allelic diversity of human leukocyte antigen (HLA) class II genes may help maintain humoral immunity against infectious diseases. In this study, we investigated germline genetic variation in classical HLA class II genes and employed a systematic, unbiased approach to explore the relative contribution of this genetic variation in the antibody repertoire to various common pathogens. We leveraged a well-defined cohort of 800 adults representing the general Arab population in which genetic material is shared because of the high frequency of consanguineous unions. By applying a high-throughput method for large-scale antibody profiling to this well-defined cohort, we were able to dissect the overall effect of zygosity for classical HLA class II genes, as well as the effects associated with specific HLA class II alleles, haplotypes and genotypes, on the antimicrobial antibody repertoire breadth and antibody specificity with unprecedented resolution. Our population genetic studies revealed that zygosity of the classical HLA class II genes is a strong predictor of antibody responses to common human pathogens, suggesting that classical HLA class II gene heterozygosity confers a selective advantage. Moreover, we demonstrated that multiple HLA class II alleles can have additive effects on the antibody repertoire to common pathogens. We also identified associations of HLA-DRB1 genotypes with specific antigens. Our findings suggest that HLA class II gene polymorphisms confer specific humoral immunity against common pathogens, which may have contributed to the genetic diversity of HLA class II loci during hominine evolution.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Frontiers in Immunology<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fimmu.2022.856497" target="_blank">https://dx.doi.org/10.3389/fimmu.2022.856497</a></p>2022-08-08T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fimmu.2022.856497https://figshare.com/articles/journal_contribution/Human_leukocyte_antigen_class_II_gene_diversity_tunes_antibody_repertoires_to_common_pathogens/25658877CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/256588772022-08-08T03:00:00Z |
| spellingShingle | Human leukocyte antigen class II gene diversity tunes antibody repertoires to common pathogens Taushif Khan (691254) Biomedical and clinical sciences Immunology human antibody repertoires microbial infection phage immunoprecipitation sequencing human leukocyte antigen major histocompatibility complex polymorphisms allelic diversity association study |
| status_str | publishedVersion |
| title | Human leukocyte antigen class II gene diversity tunes antibody repertoires to common pathogens |
| title_full | Human leukocyte antigen class II gene diversity tunes antibody repertoires to common pathogens |
| title_fullStr | Human leukocyte antigen class II gene diversity tunes antibody repertoires to common pathogens |
| title_full_unstemmed | Human leukocyte antigen class II gene diversity tunes antibody repertoires to common pathogens |
| title_short | Human leukocyte antigen class II gene diversity tunes antibody repertoires to common pathogens |
| title_sort | Human leukocyte antigen class II gene diversity tunes antibody repertoires to common pathogens |
| topic | Biomedical and clinical sciences Immunology human antibody repertoires microbial infection phage immunoprecipitation sequencing human leukocyte antigen major histocompatibility complex polymorphisms allelic diversity association study |