Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients
<p dir="ltr">Transient ischemic attack (TIA) refers to a momentary neurologic deficit caused by focal cerebral, spinal or retinal ischemic insult. TIA is associated with a high risk of impending acute ischemic stroke (AIS), a neurologic dysfunction characterized by focal cerebral, sp...
محفوظ في:
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| مؤلفون آخرون: | , , , , , , , , , , , , , |
| منشور في: |
2022
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| _version_ | 1864513511883800576 |
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| author | Salman M. Toor (8854751) |
| author2 | Eman K. Aldous (13914057) Aijaz Parray (290256) Naveed Akhtar (4919398) Yasser Al-Sarraj (11721425) Essam M. Abdelalim (5768072) Abdelilah Arredouani (10914455) Omar El-Agnaf (2134222) Paul J. Thornalley (291723) Sajitha V. Pananchikkal (13914060) Ghulam Jeelani Pir (13914063) Raheem Ayadathil (14150124) Ashfaq Shuaib (4919407) Nehad M. Alajez (7397276) Omar M. E. Albagha (11704871) |
| author2_role | author author author author author author author author author author author author author author |
| author_facet | Salman M. Toor (8854751) Eman K. Aldous (13914057) Aijaz Parray (290256) Naveed Akhtar (4919398) Yasser Al-Sarraj (11721425) Essam M. Abdelalim (5768072) Abdelilah Arredouani (10914455) Omar El-Agnaf (2134222) Paul J. Thornalley (291723) Sajitha V. Pananchikkal (13914060) Ghulam Jeelani Pir (13914063) Raheem Ayadathil (14150124) Ashfaq Shuaib (4919407) Nehad M. Alajez (7397276) Omar M. E. Albagha (11704871) |
| author_role | author |
| dc.creator.none.fl_str_mv | Salman M. Toor (8854751) Eman K. Aldous (13914057) Aijaz Parray (290256) Naveed Akhtar (4919398) Yasser Al-Sarraj (11721425) Essam M. Abdelalim (5768072) Abdelilah Arredouani (10914455) Omar El-Agnaf (2134222) Paul J. Thornalley (291723) Sajitha V. Pananchikkal (13914060) Ghulam Jeelani Pir (13914063) Raheem Ayadathil (14150124) Ashfaq Shuaib (4919407) Nehad M. Alajez (7397276) Omar M. E. Albagha (11704871) |
| dc.date.none.fl_str_mv | 2022-12-21T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/ijms24010108 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Circulating_MicroRNA_Profiling_Identifies_Distinct_MicroRNA_Signatures_in_Acute_Ischemic_Stroke_and_Transient_Ischemic_Attack_Patients/26095201 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Bioinformatics and computational biology Genetics Biomedical and clinical sciences Cardiovascular medicine and haematology Neurosciences microRNA miRNome schemic stroke acute ischemic stroke transient ischemic attack |
| dc.title.none.fl_str_mv | Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Transient ischemic attack (TIA) refers to a momentary neurologic deficit caused by focal cerebral, spinal or retinal ischemic insult. TIA is associated with a high risk of impending acute ischemic stroke (AIS), a neurologic dysfunction characterized by focal cerebral, spinal or retinal infarction. Understanding the differences in molecular pathways in AIS and TIA has merit for deciphering the underlying cause for neuronal deficits with long-term effects and high risks of morbidity and mortality. In this study, we performed comprehensive investigations into the circulating microRNA (miRNA) profiles of AIS (n = 191) and TIA (n = 61) patients. We performed RNA-Seq on serum samples collected within 24 hrs of clinical diagnosis and randomly divided the study populations into discovery and validation cohorts. We identified a panel of 11 differentially regulated miRNAs at FDR < 0.05. Hsa-miR-548c-5p, -20a-5p, -18a-5p, -484, -652-3p, -486-3p, -24-3p, -181a-5p and -222-3p were upregulated, while hsa-miR-500a-3p and -206 were downregulated in AIS patients compared to TIA patients. We also probed the previously validated gene targets of our identified miRNA panel to highlight the molecular pathways affected in AIS. Moreover, we developed a multivariate classifier with potential utilization as a discriminative biomarker for AIS and TIA patients. The underlying molecular pathways in AIS compared to TIA may be explored further in functional studies for therapeutic targeting in clinical translation.</p><h2>Other Information</h2><p dir="ltr">Published in: International Journal of Molecular Sciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/ijms24010108" target="_blank">https://dx.doi.org/10.3390/ijms24010108</a></p><p dir="ltr">Additional institutions affiliated with: Qatar Genome Programme (2015-2024), Qatar Precision Health Institute.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_2c286997d072b8b0814c6eede16fb139 |
| identifier_str_mv | 10.3390/ijms24010108 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26095201 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack PatientsSalman M. Toor (8854751)Eman K. Aldous (13914057)Aijaz Parray (290256)Naveed Akhtar (4919398)Yasser Al-Sarraj (11721425)Essam M. Abdelalim (5768072)Abdelilah Arredouani (10914455)Omar El-Agnaf (2134222)Paul J. Thornalley (291723)Sajitha V. Pananchikkal (13914060)Ghulam Jeelani Pir (13914063)Raheem Ayadathil (14150124)Ashfaq Shuaib (4919407)Nehad M. Alajez (7397276)Omar M. E. Albagha (11704871)Biological sciencesBioinformatics and computational biologyGeneticsBiomedical and clinical sciencesCardiovascular medicine and haematologyNeurosciencesmicroRNAmiRNomeschemicstrokeacute ischemic stroketransient ischemic attack<p dir="ltr">Transient ischemic attack (TIA) refers to a momentary neurologic deficit caused by focal cerebral, spinal or retinal ischemic insult. TIA is associated with a high risk of impending acute ischemic stroke (AIS), a neurologic dysfunction characterized by focal cerebral, spinal or retinal infarction. Understanding the differences in molecular pathways in AIS and TIA has merit for deciphering the underlying cause for neuronal deficits with long-term effects and high risks of morbidity and mortality. In this study, we performed comprehensive investigations into the circulating microRNA (miRNA) profiles of AIS (n = 191) and TIA (n = 61) patients. We performed RNA-Seq on serum samples collected within 24 hrs of clinical diagnosis and randomly divided the study populations into discovery and validation cohorts. We identified a panel of 11 differentially regulated miRNAs at FDR < 0.05. Hsa-miR-548c-5p, -20a-5p, -18a-5p, -484, -652-3p, -486-3p, -24-3p, -181a-5p and -222-3p were upregulated, while hsa-miR-500a-3p and -206 were downregulated in AIS patients compared to TIA patients. We also probed the previously validated gene targets of our identified miRNA panel to highlight the molecular pathways affected in AIS. Moreover, we developed a multivariate classifier with potential utilization as a discriminative biomarker for AIS and TIA patients. The underlying molecular pathways in AIS compared to TIA may be explored further in functional studies for therapeutic targeting in clinical translation.</p><h2>Other Information</h2><p dir="ltr">Published in: International Journal of Molecular Sciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/ijms24010108" target="_blank">https://dx.doi.org/10.3390/ijms24010108</a></p><p dir="ltr">Additional institutions affiliated with: Qatar Genome Programme (2015-2024), Qatar Precision Health Institute.</p>2022-12-21T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/ijms24010108https://figshare.com/articles/journal_contribution/Circulating_MicroRNA_Profiling_Identifies_Distinct_MicroRNA_Signatures_in_Acute_Ischemic_Stroke_and_Transient_Ischemic_Attack_Patients/26095201CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/260952012022-12-21T03:00:00Z |
| spellingShingle | Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients Salman M. Toor (8854751) Biological sciences Bioinformatics and computational biology Genetics Biomedical and clinical sciences Cardiovascular medicine and haematology Neurosciences microRNA miRNome schemic stroke acute ischemic stroke transient ischemic attack |
| status_str | publishedVersion |
| title | Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients |
| title_full | Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients |
| title_fullStr | Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients |
| title_full_unstemmed | Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients |
| title_short | Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients |
| title_sort | Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients |
| topic | Biological sciences Bioinformatics and computational biology Genetics Biomedical and clinical sciences Cardiovascular medicine and haematology Neurosciences microRNA miRNome schemic stroke acute ischemic stroke transient ischemic attack |