Myeloid cells in circulation and tumor microenvironment of breast cancer patients
<p dir="ltr">Pathological conditions including cancers lead to accumulation of a morphological mixture of highly immunosuppressive cells termed as myeloid-derived suppressor cells (MDSC). The lack of conclusive markers to identify human MDSC, due to their heterogeneous nature and clo...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , , |
| Published: |
2017
|
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1864513557036531712 |
|---|---|
| author | Salman M. Toor (8854751) |
| author2 | Azharuddin Sajid Syed Khaja (19720114) Haytham El Salhat (5396387) Issam Faour (19720108) Jihad Kanbar (19720192) Asif A. Quadri (19720195) Mohamed Albashir (19720198) Eyad Elkord (5396390) |
| author2_role | author author author author author author author |
| author_facet | Salman M. Toor (8854751) Azharuddin Sajid Syed Khaja (19720114) Haytham El Salhat (5396387) Issam Faour (19720108) Jihad Kanbar (19720192) Asif A. Quadri (19720195) Mohamed Albashir (19720198) Eyad Elkord (5396390) |
| author_role | author |
| dc.creator.none.fl_str_mv | Salman M. Toor (8854751) Azharuddin Sajid Syed Khaja (19720114) Haytham El Salhat (5396387) Issam Faour (19720108) Jihad Kanbar (19720192) Asif A. Quadri (19720195) Mohamed Albashir (19720198) Eyad Elkord (5396390) |
| dc.date.none.fl_str_mv | 2017-03-10T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1007/s00262-017-1977-z |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Myeloid_cells_in_circulation_and_tumor_microenvironment_of_breast_cancer_patients/27082780 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Immunology Oncology and carcinogenesis Myeloid cells Myeloid-derived suppressor cells Neutrophils Breast cancer Tumor microenvironment Circulation |
| dc.title.none.fl_str_mv | Myeloid cells in circulation and tumor microenvironment of breast cancer patients |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Pathological conditions including cancers lead to accumulation of a morphological mixture of highly immunosuppressive cells termed as myeloid-derived suppressor cells (MDSC). The lack of conclusive markers to identify human MDSC, due to their heterogeneous nature and close phenotypical and functional proximity with other cell subsets, made it challenging to identify these cells. Nevertheless, expansion of MDSC has been reported in periphery and tumor microenvironment of various cancers. The majority of studies on breast cancers were performed on murine models and hence limited literature is available on the relation of MDSC accumulation with clinical settings in breast cancer patients. The aim of this study was to investigate levels and phenotypes of myeloid cells in peripheral blood (<i>n</i> = 23) and tumor microenvironment of primary breast cancer patients (<i>n</i> = 7), compared with blood from healthy donors (<i>n</i> = 21) and paired non-tumor normal breast tissues from the same patients (<i>n</i> = 7). Using multicolor flow cytometric assays, we found that breast cancer patients had significantly higher levels of tumor-infiltrating myeloid cells, which comprised of granulocytes (<i>P</i> = 0.022) and immature cells that lack the expression of markers for fully differentiated monocytes or granulocytes (<i>P</i> = 0.016). Importantly, this expansion was not reflected in the peripheral blood. The immunosuppressive potential of these cells was confirmed by expression of Arginase 1 (ARG1), which is pivotal for T-cell suppression. These findings are important for developing therapeutic modalities to target mechanisms employed by immunosuppressive cells that generate an immune-permissive environment for the progression of cancer.</p><h2>Other Information</h2><p dir="ltr">Published in: Cancer Immunology, Immunotherapy<br>License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s00262-017-1977-z" target="_blank">https://dx.doi.org/10.1007/s00262-017-1977-z</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_2ffa6b4a4e9d2aa87b5714777cc9c1bd |
| identifier_str_mv | 10.1007/s00262-017-1977-z |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/27082780 |
| publishDate | 2017 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Myeloid cells in circulation and tumor microenvironment of breast cancer patientsSalman M. Toor (8854751)Azharuddin Sajid Syed Khaja (19720114)Haytham El Salhat (5396387)Issam Faour (19720108)Jihad Kanbar (19720192)Asif A. Quadri (19720195)Mohamed Albashir (19720198)Eyad Elkord (5396390)Biomedical and clinical sciencesImmunologyOncology and carcinogenesisMyeloid cellsMyeloid-derived suppressor cellsNeutrophilsBreast cancerTumor microenvironmentCirculation<p dir="ltr">Pathological conditions including cancers lead to accumulation of a morphological mixture of highly immunosuppressive cells termed as myeloid-derived suppressor cells (MDSC). The lack of conclusive markers to identify human MDSC, due to their heterogeneous nature and close phenotypical and functional proximity with other cell subsets, made it challenging to identify these cells. Nevertheless, expansion of MDSC has been reported in periphery and tumor microenvironment of various cancers. The majority of studies on breast cancers were performed on murine models and hence limited literature is available on the relation of MDSC accumulation with clinical settings in breast cancer patients. The aim of this study was to investigate levels and phenotypes of myeloid cells in peripheral blood (<i>n</i> = 23) and tumor microenvironment of primary breast cancer patients (<i>n</i> = 7), compared with blood from healthy donors (<i>n</i> = 21) and paired non-tumor normal breast tissues from the same patients (<i>n</i> = 7). Using multicolor flow cytometric assays, we found that breast cancer patients had significantly higher levels of tumor-infiltrating myeloid cells, which comprised of granulocytes (<i>P</i> = 0.022) and immature cells that lack the expression of markers for fully differentiated monocytes or granulocytes (<i>P</i> = 0.016). Importantly, this expansion was not reflected in the peripheral blood. The immunosuppressive potential of these cells was confirmed by expression of Arginase 1 (ARG1), which is pivotal for T-cell suppression. These findings are important for developing therapeutic modalities to target mechanisms employed by immunosuppressive cells that generate an immune-permissive environment for the progression of cancer.</p><h2>Other Information</h2><p dir="ltr">Published in: Cancer Immunology, Immunotherapy<br>License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s00262-017-1977-z" target="_blank">https://dx.doi.org/10.1007/s00262-017-1977-z</a></p>2017-03-10T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s00262-017-1977-zhttps://figshare.com/articles/journal_contribution/Myeloid_cells_in_circulation_and_tumor_microenvironment_of_breast_cancer_patients/27082780CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/270827802017-03-10T03:00:00Z |
| spellingShingle | Myeloid cells in circulation and tumor microenvironment of breast cancer patients Salman M. Toor (8854751) Biomedical and clinical sciences Immunology Oncology and carcinogenesis Myeloid cells Myeloid-derived suppressor cells Neutrophils Breast cancer Tumor microenvironment Circulation |
| status_str | publishedVersion |
| title | Myeloid cells in circulation and tumor microenvironment of breast cancer patients |
| title_full | Myeloid cells in circulation and tumor microenvironment of breast cancer patients |
| title_fullStr | Myeloid cells in circulation and tumor microenvironment of breast cancer patients |
| title_full_unstemmed | Myeloid cells in circulation and tumor microenvironment of breast cancer patients |
| title_short | Myeloid cells in circulation and tumor microenvironment of breast cancer patients |
| title_sort | Myeloid cells in circulation and tumor microenvironment of breast cancer patients |
| topic | Biomedical and clinical sciences Immunology Oncology and carcinogenesis Myeloid cells Myeloid-derived suppressor cells Neutrophils Breast cancer Tumor microenvironment Circulation |