Comprehensive analysis of LncRNAs expression profiles in an in vitro model of steatosis treated with Exendin-4
<h3>Background and aims</h3><p dir="ltr">The hallmark of non-alcoholic fatty liver disease (NAFLD) is the excessive hepatic lipid accumulation. Currently, no pharmacotherapy exists for NAFLD. However, the glucagon-like peptide-1 receptor agonists have recently emerged as...
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2021
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| _version_ | 1864513541050990592 |
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| author | Khaoula Errafii (10914446) |
| author2 | Neyla S. Al-Akl (10914449) Olfa Khalifa (10914452) Abdelilah Arredouani (14070786) |
| author2_role | author author author |
| author_facet | Khaoula Errafii (10914446) Neyla S. Al-Akl (10914449) Olfa Khalifa (10914452) Abdelilah Arredouani (14070786) |
| author_role | author |
| dc.creator.none.fl_str_mv | Khaoula Errafii (10914446) Neyla S. Al-Akl (10914449) Olfa Khalifa (10914452) Abdelilah Arredouani (14070786) |
| dc.date.none.fl_str_mv | 2021-06-02T18:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1186/s12967-021-02885-4 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Comprehensive_analysis_of_LncRNAs_expression_profiles_in_an_in_vitro_model_of_steatosis_treated_with_Exendin-4/21598452 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Medical biochemistry and metabolomics General Biochemistry, Genetics and Molecular Biology General Medicine |
| dc.title.none.fl_str_mv | Comprehensive analysis of LncRNAs expression profiles in an in vitro model of steatosis treated with Exendin-4 |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Background and aims</h3><p dir="ltr">The hallmark of non-alcoholic fatty liver disease (NAFLD) is the excessive hepatic lipid accumulation. Currently, no pharmacotherapy exists for NAFLD. However, the glucagon-like peptide-1 receptor agonists have recently emerged as potential therapeutics. Here, we sought to identify the long non-coding RNAs (LncRNAs) associated with the steatosis improvement induced by the GLP-1R agonist Exendin-4 (Ex-4) in vitro.</p><h3>Methods</h3><p dir="ltr">Steatosis was induced in HepG2 cells with oleic acid. The transcriptomic profiling was performed using total RNA extracted from untreated, steatotic, and Ex-4-treated steatotic cells. We validated a subset of differentially expressed LncRNAs with qRT-PCR and identified the most significantly enriched cellular functions associated with the relevant LncRNAs.</p><h3>Results</h3><p dir="ltr">We confirm that Ex-4 improves steatosis in HepG2 cells. We found 379 and 180 differentially expressed LncRNAs between untreated and steatotic cells and between steatotic and Ex-4-treated steatotic cells, respectively. Interestingly, 22 upregulated LncRNAs in steatotic cells became downregulated with Ex-4 exposure, while 50 downregulated LncRNAs in steatotic cells became upregulated in the presence of Ex-4. Although some LncRNAs, such as MALAT1, H19, and NEAT1, were previously associated with NAFLD, the association of others with steatosis and the positive effect of Ex-4 is being reported for the first time. Functional enrichment analysis identified many critical pathways, including fatty acid and pyruvate metabolism, and insulin, PPAR, Wnt, TGF-β, mTOR, VEGF, NOD-like, and Toll-like receptors signaling pathways.</p><h3>Conclusion</h3><p dir="ltr">Our results suggest that LncRNAs may play essential roles in the mechanisms underlying steatosis improvement in response to GLP-1R agonists and warrant further functional studies.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Translational Medicine<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1186/s12967-021-02885-4" target="_blank">http://dx.doi.org/10.1186/s12967-021-02885-4</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_322a235324460a4f43140843d85ea46a |
| identifier_str_mv | 10.1186/s12967-021-02885-4 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/21598452 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Comprehensive analysis of LncRNAs expression profiles in an in vitro model of steatosis treated with Exendin-4Khaoula Errafii (10914446)Neyla S. Al-Akl (10914449)Olfa Khalifa (10914452)Abdelilah Arredouani (14070786)Biological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesMedical biochemistry and metabolomicsGeneral Biochemistry, Genetics and Molecular BiologyGeneral Medicine<h3>Background and aims</h3><p dir="ltr">The hallmark of non-alcoholic fatty liver disease (NAFLD) is the excessive hepatic lipid accumulation. Currently, no pharmacotherapy exists for NAFLD. However, the glucagon-like peptide-1 receptor agonists have recently emerged as potential therapeutics. Here, we sought to identify the long non-coding RNAs (LncRNAs) associated with the steatosis improvement induced by the GLP-1R agonist Exendin-4 (Ex-4) in vitro.</p><h3>Methods</h3><p dir="ltr">Steatosis was induced in HepG2 cells with oleic acid. The transcriptomic profiling was performed using total RNA extracted from untreated, steatotic, and Ex-4-treated steatotic cells. We validated a subset of differentially expressed LncRNAs with qRT-PCR and identified the most significantly enriched cellular functions associated with the relevant LncRNAs.</p><h3>Results</h3><p dir="ltr">We confirm that Ex-4 improves steatosis in HepG2 cells. We found 379 and 180 differentially expressed LncRNAs between untreated and steatotic cells and between steatotic and Ex-4-treated steatotic cells, respectively. Interestingly, 22 upregulated LncRNAs in steatotic cells became downregulated with Ex-4 exposure, while 50 downregulated LncRNAs in steatotic cells became upregulated in the presence of Ex-4. Although some LncRNAs, such as MALAT1, H19, and NEAT1, were previously associated with NAFLD, the association of others with steatosis and the positive effect of Ex-4 is being reported for the first time. Functional enrichment analysis identified many critical pathways, including fatty acid and pyruvate metabolism, and insulin, PPAR, Wnt, TGF-β, mTOR, VEGF, NOD-like, and Toll-like receptors signaling pathways.</p><h3>Conclusion</h3><p dir="ltr">Our results suggest that LncRNAs may play essential roles in the mechanisms underlying steatosis improvement in response to GLP-1R agonists and warrant further functional studies.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Translational Medicine<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1186/s12967-021-02885-4" target="_blank">http://dx.doi.org/10.1186/s12967-021-02885-4</a></p>2021-06-02T18:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1186/s12967-021-02885-4https://figshare.com/articles/journal_contribution/Comprehensive_analysis_of_LncRNAs_expression_profiles_in_an_in_vitro_model_of_steatosis_treated_with_Exendin-4/21598452CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/215984522021-06-02T18:00:00Z |
| spellingShingle | Comprehensive analysis of LncRNAs expression profiles in an in vitro model of steatosis treated with Exendin-4 Khaoula Errafii (10914446) Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Medical biochemistry and metabolomics General Biochemistry, Genetics and Molecular Biology General Medicine |
| status_str | publishedVersion |
| title | Comprehensive analysis of LncRNAs expression profiles in an in vitro model of steatosis treated with Exendin-4 |
| title_full | Comprehensive analysis of LncRNAs expression profiles in an in vitro model of steatosis treated with Exendin-4 |
| title_fullStr | Comprehensive analysis of LncRNAs expression profiles in an in vitro model of steatosis treated with Exendin-4 |
| title_full_unstemmed | Comprehensive analysis of LncRNAs expression profiles in an in vitro model of steatosis treated with Exendin-4 |
| title_short | Comprehensive analysis of LncRNAs expression profiles in an in vitro model of steatosis treated with Exendin-4 |
| title_sort | Comprehensive analysis of LncRNAs expression profiles in an in vitro model of steatosis treated with Exendin-4 |
| topic | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Medical biochemistry and metabolomics General Biochemistry, Genetics and Molecular Biology General Medicine |