Minor Intron Splicing from Basic Science to Disease

<p dir="ltr">Pre-mRNA splicing is an essential step in gene expression and is catalyzed by two machineries in eukaryotes: the major (U2 type) and minor (U12 type) spliceosomes. While the majority of introns in humans are U2 type, less than 0.4% are U12 type, also known as minor intro...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Ettaib El Marabti (23276158) (author)
مؤلفون آخرون: Joel Malek (3497978) (author), Ihab Younis (57824) (author)
منشور في: 2021
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author Ettaib El Marabti (23276158)
author2 Joel Malek (3497978)
Ihab Younis (57824)
author2_role author
author
author_facet Ettaib El Marabti (23276158)
Joel Malek (3497978)
Ihab Younis (57824)
author_role author
dc.creator.none.fl_str_mv Ettaib El Marabti (23276158)
Joel Malek (3497978)
Ihab Younis (57824)
dc.date.none.fl_str_mv 2021-06-04T03:00:00Z
dc.identifier.none.fl_str_mv 10.3390/ijms22116062
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Minor_Intron_Splicing_from_Basic_Science_to_Disease/31444717
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biological sciences
Biochemistry and cell biology
Genetics
minor introns
U2 introns
U12 introns
minor spliceosome
RNA splicing
disease
dc.title.none.fl_str_mv Minor Intron Splicing from Basic Science to Disease
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Pre-mRNA splicing is an essential step in gene expression and is catalyzed by two machineries in eukaryotes: the major (U2 type) and minor (U12 type) spliceosomes. While the majority of introns in humans are U2 type, less than 0.4% are U12 type, also known as minor introns (mi-INTs), and require a specialized spliceosome composed of U11, U12, U4atac, U5, and U6atac snRNPs. The high evolutionary conservation and apparent splicing inefficiency of U12 introns have set them apart from their major counterparts and led to speculations on the purpose for their existence. However, recent studies challenged the simple concept of mi-INTs splicing inefficiency due to low abundance of their spliceosome and confirmed their regulatory role in alternative splicing, significantly impacting the expression of their host genes. Additionally, a growing list of minor spliceosome-associated diseases with tissue-specific pathologies affirmed the importance of minor splicing as a key regulatory pathway, which when deregulated could lead to tissue-specific pathologies due to specific alterations in the expression of some minor-intron-containing genes. Consequently, uncovering how mi-INTs splicing is regulated in a tissue-specific manner would allow for better understanding of disease pathogenesis and pave the way for novel therapies, which we highlight in this review.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: International Journal of Molecular Sciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/ijms22116062" target="_blank">https://dx.doi.org/10.3390/ijms22116062</a></p>
eu_rights_str_mv openAccess
id Manara2_34294fec082036cd8f994b1022afdc1d
identifier_str_mv 10.3390/ijms22116062
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/31444717
publishDate 2021
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spelling Minor Intron Splicing from Basic Science to DiseaseEttaib El Marabti (23276158)Joel Malek (3497978)Ihab Younis (57824)Biological sciencesBiochemistry and cell biologyGeneticsminor intronsU2 intronsU12 intronsminor spliceosomeRNA splicingdisease<p dir="ltr">Pre-mRNA splicing is an essential step in gene expression and is catalyzed by two machineries in eukaryotes: the major (U2 type) and minor (U12 type) spliceosomes. While the majority of introns in humans are U2 type, less than 0.4% are U12 type, also known as minor introns (mi-INTs), and require a specialized spliceosome composed of U11, U12, U4atac, U5, and U6atac snRNPs. The high evolutionary conservation and apparent splicing inefficiency of U12 introns have set them apart from their major counterparts and led to speculations on the purpose for their existence. However, recent studies challenged the simple concept of mi-INTs splicing inefficiency due to low abundance of their spliceosome and confirmed their regulatory role in alternative splicing, significantly impacting the expression of their host genes. Additionally, a growing list of minor spliceosome-associated diseases with tissue-specific pathologies affirmed the importance of minor splicing as a key regulatory pathway, which when deregulated could lead to tissue-specific pathologies due to specific alterations in the expression of some minor-intron-containing genes. Consequently, uncovering how mi-INTs splicing is regulated in a tissue-specific manner would allow for better understanding of disease pathogenesis and pave the way for novel therapies, which we highlight in this review.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: International Journal of Molecular Sciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/ijms22116062" target="_blank">https://dx.doi.org/10.3390/ijms22116062</a></p>2021-06-04T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/ijms22116062https://figshare.com/articles/journal_contribution/Minor_Intron_Splicing_from_Basic_Science_to_Disease/31444717CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/314447172021-06-04T03:00:00Z
spellingShingle Minor Intron Splicing from Basic Science to Disease
Ettaib El Marabti (23276158)
Biological sciences
Biochemistry and cell biology
Genetics
minor introns
U2 introns
U12 introns
minor spliceosome
RNA splicing
disease
status_str publishedVersion
title Minor Intron Splicing from Basic Science to Disease
title_full Minor Intron Splicing from Basic Science to Disease
title_fullStr Minor Intron Splicing from Basic Science to Disease
title_full_unstemmed Minor Intron Splicing from Basic Science to Disease
title_short Minor Intron Splicing from Basic Science to Disease
title_sort Minor Intron Splicing from Basic Science to Disease
topic Biological sciences
Biochemistry and cell biology
Genetics
minor introns
U2 introns
U12 introns
minor spliceosome
RNA splicing
disease