Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar
<p dir="ltr">Acute gastroenteritis (AGE) is associated with significant global morbidity and mortality, especially among children under five years of age. Viruses are well established as etiologic agents of gastroenteritis since they are the most common pathogens that contribute to t...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , , |
| منشور في: |
2022
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| الموضوعات: | |
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إضافة وسم
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| _version_ | 1864513540501536768 |
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| author | Ghina Hijazi (17346904) |
| author2 | Fatima Dakroub (13014998) Pierre Khoueiry (258758) Abdullah El-Kurdi (17346907) Amani Ezzeddine (17346910) Habib Alkalamouni (17283355) Khalid Alansari (17346913) Asmaa A. Althani (4675855) Shilu Mathew (625826) Hebah A. AlKhatib (17346916) Hadi M. Yassine (4675846) Hassan Zaraket (194276) |
| author2_role | author author author author author author author author author author author |
| author_facet | Ghina Hijazi (17346904) Fatima Dakroub (13014998) Pierre Khoueiry (258758) Abdullah El-Kurdi (17346907) Amani Ezzeddine (17346910) Habib Alkalamouni (17283355) Khalid Alansari (17346913) Asmaa A. Althani (4675855) Shilu Mathew (625826) Hebah A. AlKhatib (17346916) Hadi M. Yassine (4675846) Hassan Zaraket (194276) |
| author_role | author |
| dc.creator.none.fl_str_mv | Ghina Hijazi (17346904) Fatima Dakroub (13014998) Pierre Khoueiry (258758) Abdullah El-Kurdi (17346907) Amani Ezzeddine (17346910) Habib Alkalamouni (17283355) Khalid Alansari (17346913) Asmaa A. Althani (4675855) Shilu Mathew (625826) Hebah A. AlKhatib (17346916) Hadi M. Yassine (4675846) Hassan Zaraket (194276) |
| dc.date.none.fl_str_mv | 2022-11-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.meegid.2022.105367 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Viral_metagenomics_analysis_of_stool_specimens_from_children_with_unresolved_gastroenteritis_in_Qatar/24516469 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Biomedical and clinical sciences Clinical sciences Gastroenteritis Viral metagenomic Next-generation sequencing Unknown etiology Viruses |
| dc.title.none.fl_str_mv | Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Acute gastroenteritis (AGE) is associated with significant global morbidity and mortality, especially among children under five years of age. Viruses are well established as etiologic agents of gastroenteritis since they are the most common pathogens that contribute to the disease burden in developing countries. Despite the advances in molecular diagnosis, a substantial proportion of AGE etiology remain unresolved. We implemented a viral metagenomics pipeline to determine the potential viral etiology associated with AGE among children under the age of five years in Qatar with undiagnosed etiology. Following enriching for the viral genome, ∼1.3 billion sequences were generated from 89 stool specimens using the Illumina HiSeq platform, of which 7% were mapped to viral genomes. Human viruses were detected in 34 specimens (38.2%); 14 were adenovirus, nine coxsackievirus A16, five rotavirus (G9P[8] and G4P[8]), four norovirus (GII), one influenza A virus (H3), and one respiratory syncytial virus A (RSVA). In conclusion, the viral metagenomics approach is useful for determining AGE's etiology when routine molecular diagnostic assays fail.</p><h2>Other Information</h2><p dir="ltr">Published in: Infection, Genetics and Evolution<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.meegid.2022.105367" target="_blank">https://dx.doi.org/10.1016/j.meegid.2022.105367</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_35c05d67e01cb98fee844194b34382e2 |
| identifier_str_mv | 10.1016/j.meegid.2022.105367 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/24516469 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in QatarGhina Hijazi (17346904)Fatima Dakroub (13014998)Pierre Khoueiry (258758)Abdullah El-Kurdi (17346907)Amani Ezzeddine (17346910)Habib Alkalamouni (17283355)Khalid Alansari (17346913)Asmaa A. Althani (4675855)Shilu Mathew (625826)Hebah A. AlKhatib (17346916)Hadi M. Yassine (4675846)Hassan Zaraket (194276)Biological sciencesGeneticsBiomedical and clinical sciencesClinical sciencesGastroenteritisViral metagenomicNext-generation sequencingUnknown etiologyViruses<p dir="ltr">Acute gastroenteritis (AGE) is associated with significant global morbidity and mortality, especially among children under five years of age. Viruses are well established as etiologic agents of gastroenteritis since they are the most common pathogens that contribute to the disease burden in developing countries. Despite the advances in molecular diagnosis, a substantial proportion of AGE etiology remain unresolved. We implemented a viral metagenomics pipeline to determine the potential viral etiology associated with AGE among children under the age of five years in Qatar with undiagnosed etiology. Following enriching for the viral genome, ∼1.3 billion sequences were generated from 89 stool specimens using the Illumina HiSeq platform, of which 7% were mapped to viral genomes. Human viruses were detected in 34 specimens (38.2%); 14 were adenovirus, nine coxsackievirus A16, five rotavirus (G9P[8] and G4P[8]), four norovirus (GII), one influenza A virus (H3), and one respiratory syncytial virus A (RSVA). In conclusion, the viral metagenomics approach is useful for determining AGE's etiology when routine molecular diagnostic assays fail.</p><h2>Other Information</h2><p dir="ltr">Published in: Infection, Genetics and Evolution<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.meegid.2022.105367" target="_blank">https://dx.doi.org/10.1016/j.meegid.2022.105367</a></p>2022-11-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.meegid.2022.105367https://figshare.com/articles/journal_contribution/Viral_metagenomics_analysis_of_stool_specimens_from_children_with_unresolved_gastroenteritis_in_Qatar/24516469CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/245164692022-11-01T00:00:00Z |
| spellingShingle | Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar Ghina Hijazi (17346904) Biological sciences Genetics Biomedical and clinical sciences Clinical sciences Gastroenteritis Viral metagenomic Next-generation sequencing Unknown etiology Viruses |
| status_str | publishedVersion |
| title | Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar |
| title_full | Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar |
| title_fullStr | Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar |
| title_full_unstemmed | Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar |
| title_short | Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar |
| title_sort | Viral metagenomics analysis of stool specimens from children with unresolved gastroenteritis in Qatar |
| topic | Biological sciences Genetics Biomedical and clinical sciences Clinical sciences Gastroenteritis Viral metagenomic Next-generation sequencing Unknown etiology Viruses |