Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac Function
<p dir="ltr">Calmodulin (CaM) is a ubiquitous, small cytosolic calcium (Ca<sup>2+</sup>)‐binding sensor that plays a vital role in many cellular processes by binding and regulating the activity of over 300 protein targets. In cardiac muscle, CaM modulates directly or indi...
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2024
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| author | Sahar I. Da'as (17128747) |
| author2 | Angelos Thanassoulas (2822861) Brian L. Calver (17807603) Alaaeldin Saleh (432644) Doua Abdelrahman (17280667) Waseem Hasan (17280670) Bared Safieh‐Garabedian (14778910) Iris Kontogianni (17807609) Gheyath K. Nasrallah (9200525) George Nounesis (2202139) F. Anthony Lai (17714499) Michail Nomikos (17563188) |
| author2_role | author author author author author author author author author author author |
| author_facet | Sahar I. Da'as (17128747) Angelos Thanassoulas (2822861) Brian L. Calver (17807603) Alaaeldin Saleh (432644) Doua Abdelrahman (17280667) Waseem Hasan (17280670) Bared Safieh‐Garabedian (14778910) Iris Kontogianni (17807609) Gheyath K. Nasrallah (9200525) George Nounesis (2202139) F. Anthony Lai (17714499) Michail Nomikos (17563188) |
| author_role | author |
| dc.creator.none.fl_str_mv | Sahar I. Da'as (17128747) Angelos Thanassoulas (2822861) Brian L. Calver (17807603) Alaaeldin Saleh (432644) Doua Abdelrahman (17280667) Waseem Hasan (17280670) Bared Safieh‐Garabedian (14778910) Iris Kontogianni (17807609) Gheyath K. Nasrallah (9200525) George Nounesis (2202139) F. Anthony Lai (17714499) Michail Nomikos (17563188) |
| dc.date.none.fl_str_mv | 2024-06-30T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1002/jcb.30619 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Divergent_Biochemical_Properties_and_Disparate_Impact_of_Arrhythmogenic_Calmodulin_Mutations_on_Zebrafish_Cardiac_Function/29899601 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Biomedical and clinical sciences Cardiovascular medicine and haematology arrhythmias calcium calmodulin cardiac disease zebrafish |
| dc.title.none.fl_str_mv | Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac Function |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Calmodulin (CaM) is a ubiquitous, small cytosolic calcium (Ca<sup>2+</sup>)‐binding sensor that plays a vital role in many cellular processes by binding and regulating the activity of over 300 protein targets. In cardiac muscle, CaM modulates directly or indirectly the activity of several proteins that play a key role in excitation‐contraction coupling (ECC), such as ryanodine receptor type 2 (RyR2), l‐type Ca<sup>2+</sup> (Ca<sub>v</sub>1.2), sodium (NaV1.5) and potassium (KV7.1) channels. Many recent clinical and genetic studies have reported a series of CaM mutations in patients with life‐threatening arrhythmogenic syndromes, such as long QT syndrome (LQTS) and catecholaminergic polymorphic ventricular tachycardia (CPVT). We recently showed that four arrhythmogenic CaM mutations (N98I, D132E, D134H, and Q136P) significantly reduce the binding of CaM to RyR2. Herein, we investigate in vivo functional effects of these CaM mutations on the normal zebrafish embryonic heart function by microinjecting complementary RNA corresponding to CaMN98I, CaM<sup>D132E</sup>, CaM<sup>D134H</sup>, and CaM<sup>Q136P</sup> mutants. Expression of CaM<sup>D132E</sup> and CaMD134H mutants results in significant reduction of the zebrafish heart rate, mimicking a severe form of human bradycardia, whereas expression of CaM<sup>Q136P</sup> results in an increased heart rate mimicking human ventricular tachycardia. Moreover, analysis of cardiac ventricular rhythm revealed that the CaM<sup>D132E</sup> and CaM<sup>N98I</sup> zebrafish groups display an irregular pattern of heart beating and increased amplitude in comparison to the control groups. Furthermore, circular dichroism spectroscopy experiments using recombinant CaM proteins reveals a decreased structural stability of the four mutants compared to the wild‐type CaM protein in the presence of Ca<sup>2+</sup>. Finally, Ca<sup>2+</sup>‐binding studies indicates that all CaM mutations display reduced CaM Ca<sup>2+</sup>‐binding affinities, with CaM<sup>D132E</sup> exhibiting the most prominent change. Our data suggest that CaM mutations can trigger different arrhythmogenic phenotypes through multiple and complex molecular mechanisms.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Cellular Biochemistry<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1002/jcb.30619" target="_blank">https://dx.doi.org/10.1002/jcb.30619</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_35dc6ec3cee6de01679c195ef3578b60 |
| identifier_str_mv | 10.1002/jcb.30619 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/29899601 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac FunctionSahar I. Da'as (17128747)Angelos Thanassoulas (2822861)Brian L. Calver (17807603)Alaaeldin Saleh (432644)Doua Abdelrahman (17280667)Waseem Hasan (17280670)Bared Safieh‐Garabedian (14778910)Iris Kontogianni (17807609)Gheyath K. Nasrallah (9200525)George Nounesis (2202139)F. Anthony Lai (17714499)Michail Nomikos (17563188)Biological sciencesGeneticsBiomedical and clinical sciencesCardiovascular medicine and haematologyarrhythmiascalciumcalmodulincardiac diseasezebrafish<p dir="ltr">Calmodulin (CaM) is a ubiquitous, small cytosolic calcium (Ca<sup>2+</sup>)‐binding sensor that plays a vital role in many cellular processes by binding and regulating the activity of over 300 protein targets. In cardiac muscle, CaM modulates directly or indirectly the activity of several proteins that play a key role in excitation‐contraction coupling (ECC), such as ryanodine receptor type 2 (RyR2), l‐type Ca<sup>2+</sup> (Ca<sub>v</sub>1.2), sodium (NaV1.5) and potassium (KV7.1) channels. Many recent clinical and genetic studies have reported a series of CaM mutations in patients with life‐threatening arrhythmogenic syndromes, such as long QT syndrome (LQTS) and catecholaminergic polymorphic ventricular tachycardia (CPVT). We recently showed that four arrhythmogenic CaM mutations (N98I, D132E, D134H, and Q136P) significantly reduce the binding of CaM to RyR2. Herein, we investigate in vivo functional effects of these CaM mutations on the normal zebrafish embryonic heart function by microinjecting complementary RNA corresponding to CaMN98I, CaM<sup>D132E</sup>, CaM<sup>D134H</sup>, and CaM<sup>Q136P</sup> mutants. Expression of CaM<sup>D132E</sup> and CaMD134H mutants results in significant reduction of the zebrafish heart rate, mimicking a severe form of human bradycardia, whereas expression of CaM<sup>Q136P</sup> results in an increased heart rate mimicking human ventricular tachycardia. Moreover, analysis of cardiac ventricular rhythm revealed that the CaM<sup>D132E</sup> and CaM<sup>N98I</sup> zebrafish groups display an irregular pattern of heart beating and increased amplitude in comparison to the control groups. Furthermore, circular dichroism spectroscopy experiments using recombinant CaM proteins reveals a decreased structural stability of the four mutants compared to the wild‐type CaM protein in the presence of Ca<sup>2+</sup>. Finally, Ca<sup>2+</sup>‐binding studies indicates that all CaM mutations display reduced CaM Ca<sup>2+</sup>‐binding affinities, with CaM<sup>D132E</sup> exhibiting the most prominent change. Our data suggest that CaM mutations can trigger different arrhythmogenic phenotypes through multiple and complex molecular mechanisms.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Cellular Biochemistry<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1002/jcb.30619" target="_blank">https://dx.doi.org/10.1002/jcb.30619</a></p>2024-06-30T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1002/jcb.30619https://figshare.com/articles/journal_contribution/Divergent_Biochemical_Properties_and_Disparate_Impact_of_Arrhythmogenic_Calmodulin_Mutations_on_Zebrafish_Cardiac_Function/29899601CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/298996012024-06-30T09:00:00Z |
| spellingShingle | Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac Function Sahar I. Da'as (17128747) Biological sciences Genetics Biomedical and clinical sciences Cardiovascular medicine and haematology arrhythmias calcium calmodulin cardiac disease zebrafish |
| status_str | publishedVersion |
| title | Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac Function |
| title_full | Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac Function |
| title_fullStr | Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac Function |
| title_full_unstemmed | Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac Function |
| title_short | Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac Function |
| title_sort | Divergent Biochemical Properties and Disparate Impact of Arrhythmogenic Calmodulin Mutations on Zebrafish Cardiac Function |
| topic | Biological sciences Genetics Biomedical and clinical sciences Cardiovascular medicine and haematology arrhythmias calcium calmodulin cardiac disease zebrafish |