<i>In silico </i>formulation of a next-generation multiepitope vaccine for use as a prophylactic candidate against Crimean-Congo hemorrhagic fever
<h3>Background</h3><p dir="ltr">Crimean-Congo hemorrhagic fever (CCHF) is a widespread disease transmitted to humans and livestock animals through the bite of infected ticks or close contact with infected persons’ blood, organs, or other bodily fluids. The virus is respon...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , |
| منشور في: |
2023
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| الموضوعات: | |
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| _version_ | 1864513507198763008 |
|---|---|
| author | Rahat Alam (9286527) |
| author2 | Abdus Samad (4388131) Foysal Ahammad (9286524) Suza Mohammad Nur (15219823) Ahad Amer Alsaiari (13902165) Raihan Rahman Imon (9286530) Md. Enamul Kabir Talukder (15219826) Zulkar Nain (7845458) Md. Mashiar Rahman (15219829) Farhan Mohammad (256409) Tomasz M. Karpiński (12881426) |
| author2_role | author author author author author author author author author author |
| author_facet | Rahat Alam (9286527) Abdus Samad (4388131) Foysal Ahammad (9286524) Suza Mohammad Nur (15219823) Ahad Amer Alsaiari (13902165) Raihan Rahman Imon (9286530) Md. Enamul Kabir Talukder (15219826) Zulkar Nain (7845458) Md. Mashiar Rahman (15219829) Farhan Mohammad (256409) Tomasz M. Karpiński (12881426) |
| author_role | author |
| dc.creator.none.fl_str_mv | Rahat Alam (9286527) Abdus Samad (4388131) Foysal Ahammad (9286524) Suza Mohammad Nur (15219823) Ahad Amer Alsaiari (13902165) Raihan Rahman Imon (9286530) Md. Enamul Kabir Talukder (15219826) Zulkar Nain (7845458) Md. Mashiar Rahman (15219829) Farhan Mohammad (256409) Tomasz M. Karpiński (12881426) |
| dc.date.none.fl_str_mv | 2023-02-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1186/s12916-023-02750-9 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/_i_In_silico_i_formulation_of_a_next-generation_multiepitope_vaccine_for_use_as_a_prophylactic_candidate_against_Crimean-Congo_hemorrhagic_fever/26808673 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Immunology CCHF Multiepitope vaccine Immunoinformatics Immune simulation Molecular dynamics simulation Molecular docking |
| dc.title.none.fl_str_mv | <i>In silico </i>formulation of a next-generation multiepitope vaccine for use as a prophylactic candidate against Crimean-Congo hemorrhagic fever |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Background</h3><p dir="ltr">Crimean-Congo hemorrhagic fever (CCHF) is a widespread disease transmitted to humans and livestock animals through the bite of infected ticks or close contact with infected persons’ blood, organs, or other bodily fluids. The virus is responsible for severe viral hemorrhagic fever outbreaks, with a case fatality rate of up to 40%. Despite having the highest fatality rate of the virus, a suitable treatment option or vaccination has not been developed yet. Therefore, this study aimed to formulate a multiepitope vaccine against CCHF through computational vaccine design approaches.</p><h3>Methods</h3><p dir="ltr">The glycoprotein, nucleoprotein, and RNA-dependent RNA polymerase of CCHF were utilized to determine immunodominant T- and B-cell epitopes. Subsequently, an integrative computational vaccinology approach was used to formulate a multi-epitopes vaccine candidate against the virus.</p><h3>Results</h3><p dir="ltr">After rigorous assessment, a multiepitope vaccine was constructed, which was antigenic, immunogenic, and non-allergenic with desired physicochemical properties. Molecular dynamics (MD) simulations of the vaccine-receptor complex show strong stability of the vaccine candidates to the targeted immune receptor. Additionally, the immune simulation of the vaccine candidates found that the vaccine could trigger real-life-like immune responses upon administration to humans.</p><h3>Conclusions</h3><p dir="ltr">Finally, we concluded that the formulated multiepitope vaccine candidates would provide excellent prophylactic properties against CCHF.</p><h2>Other Information</h2><p dir="ltr">Published in: BMC Medicine<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s12916-023-02750-9" target="_blank">https://dx.doi.org/10.1186/s12916-023-02750-9</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_3670f1291fdcd490c32bf43105c4a81f |
| identifier_str_mv | 10.1186/s12916-023-02750-9 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26808673 |
| publishDate | 2023 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | <i>In silico </i>formulation of a next-generation multiepitope vaccine for use as a prophylactic candidate against Crimean-Congo hemorrhagic feverRahat Alam (9286527)Abdus Samad (4388131)Foysal Ahammad (9286524)Suza Mohammad Nur (15219823)Ahad Amer Alsaiari (13902165)Raihan Rahman Imon (9286530)Md. Enamul Kabir Talukder (15219826)Zulkar Nain (7845458)Md. Mashiar Rahman (15219829)Farhan Mohammad (256409)Tomasz M. Karpiński (12881426)Biomedical and clinical sciencesClinical sciencesImmunologyCCHFMultiepitope vaccineImmunoinformaticsImmune simulationMolecular dynamics simulationMolecular docking<h3>Background</h3><p dir="ltr">Crimean-Congo hemorrhagic fever (CCHF) is a widespread disease transmitted to humans and livestock animals through the bite of infected ticks or close contact with infected persons’ blood, organs, or other bodily fluids. The virus is responsible for severe viral hemorrhagic fever outbreaks, with a case fatality rate of up to 40%. Despite having the highest fatality rate of the virus, a suitable treatment option or vaccination has not been developed yet. Therefore, this study aimed to formulate a multiepitope vaccine against CCHF through computational vaccine design approaches.</p><h3>Methods</h3><p dir="ltr">The glycoprotein, nucleoprotein, and RNA-dependent RNA polymerase of CCHF were utilized to determine immunodominant T- and B-cell epitopes. Subsequently, an integrative computational vaccinology approach was used to formulate a multi-epitopes vaccine candidate against the virus.</p><h3>Results</h3><p dir="ltr">After rigorous assessment, a multiepitope vaccine was constructed, which was antigenic, immunogenic, and non-allergenic with desired physicochemical properties. Molecular dynamics (MD) simulations of the vaccine-receptor complex show strong stability of the vaccine candidates to the targeted immune receptor. Additionally, the immune simulation of the vaccine candidates found that the vaccine could trigger real-life-like immune responses upon administration to humans.</p><h3>Conclusions</h3><p dir="ltr">Finally, we concluded that the formulated multiepitope vaccine candidates would provide excellent prophylactic properties against CCHF.</p><h2>Other Information</h2><p dir="ltr">Published in: BMC Medicine<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s12916-023-02750-9" target="_blank">https://dx.doi.org/10.1186/s12916-023-02750-9</a></p>2023-02-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1186/s12916-023-02750-9https://figshare.com/articles/journal_contribution/_i_In_silico_i_formulation_of_a_next-generation_multiepitope_vaccine_for_use_as_a_prophylactic_candidate_against_Crimean-Congo_hemorrhagic_fever/26808673CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/268086732023-02-01T00:00:00Z |
| spellingShingle | <i>In silico </i>formulation of a next-generation multiepitope vaccine for use as a prophylactic candidate against Crimean-Congo hemorrhagic fever Rahat Alam (9286527) Biomedical and clinical sciences Clinical sciences Immunology CCHF Multiepitope vaccine Immunoinformatics Immune simulation Molecular dynamics simulation Molecular docking |
| status_str | publishedVersion |
| title | <i>In silico </i>formulation of a next-generation multiepitope vaccine for use as a prophylactic candidate against Crimean-Congo hemorrhagic fever |
| title_full | <i>In silico </i>formulation of a next-generation multiepitope vaccine for use as a prophylactic candidate against Crimean-Congo hemorrhagic fever |
| title_fullStr | <i>In silico </i>formulation of a next-generation multiepitope vaccine for use as a prophylactic candidate against Crimean-Congo hemorrhagic fever |
| title_full_unstemmed | <i>In silico </i>formulation of a next-generation multiepitope vaccine for use as a prophylactic candidate against Crimean-Congo hemorrhagic fever |
| title_short | <i>In silico </i>formulation of a next-generation multiepitope vaccine for use as a prophylactic candidate against Crimean-Congo hemorrhagic fever |
| title_sort | <i>In silico </i>formulation of a next-generation multiepitope vaccine for use as a prophylactic candidate against Crimean-Congo hemorrhagic fever |
| topic | Biomedical and clinical sciences Clinical sciences Immunology CCHF Multiepitope vaccine Immunoinformatics Immune simulation Molecular dynamics simulation Molecular docking |