Antibody stability: A key to performance - Analysis, influences and improvement

<p>An antibody’s stability greatly influences its performance (i.e. its specificity and affinity). Thus, stability is a major issue for researchers and manufacturers, especially with the increasing use of antibodies in therapeutics, diagnostics and rapid analytical platforms. Here we review an...

Full description

Saved in:
Bibliographic Details
Main Author: Hui Ma (290168) (author)
Other Authors: Ciarán Ó’Fágáin (19672597) (author), Richard O’Kennedy (713891) (author)
Published: 2020
Subjects:
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<p>An antibody’s stability greatly influences its performance (i.e. its specificity and affinity). Thus, stability is a major issue for researchers and manufacturers, especially with the increasing use of antibodies in therapeutics, diagnostics and rapid analytical platforms. Here we review antibody stability under five headings: (i) measurement techniques; (ii) stability issues in expression and production (expression, proteolysis, aggregation); (iii) effects of antibody format and engineering on stability and (iv) formulation, drying and storage conditions. We consider more than 100 sources, including patents, and conclude with (v) recommendations to promote antibody stability.</p><h2>Other Information</h2> <p> Published in: Biochimie<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.biochi.2020.08.019" target="_blank">https://dx.doi.org/10.1016/j.biochi.2020.08.019</a></p>